Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,438,864	T→G	R89S (AGA→AGC)	zntR ←	zntA gene transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,438,864	0	T	G	99.0%	720.6 / ‑5.5	201	R89S (AGA→AGC)	zntR	zntA gene transcriptional activator
Reads supporting (aligned to +/- strand): ref base T (0/2); new base G (103/96); total (103/98)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

CTGCTATGAGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACTGAAACC  >  NC_000913/3438769‑3438959

cTGCTATGAGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCttt                                                                                             >  2:1898658/1‑100 (MQ=255)

cTGCTATGAGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCttt                                                                                             >  2:1928061/1‑100 (MQ=255)

cTGCTATGAGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCttt                                                                                             >  2:446241/1‑100 (MQ=255)

  gCTATGAGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTcc                                                                                           <  1:4055362/100‑1 (MQ=255)

  gCTATGAGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTcc                                                                                           <  1:356788/100‑1 (MQ=255)

  gCTATGAGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTcc                                                                                           >  2:2553596/1‑100 (MQ=255)

  gCTATGAGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTcc                                                                                           >  1:2120875/1‑100 (MQ=255)

     aTGAGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGc                                                                                        <  1:1949424/100‑1 (MQ=255)

      tGAGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGca                                                                                       >  2:500970/1‑100 (MQ=255)

       gAGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGcac                                                                                      <  1:1062642/100‑1 (MQ=255)

       gAGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGcac                                                                                      <  2:5053795/100‑1 (MQ=255)

        aGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGcaca                                                                                     >  1:5313989/1‑100 (MQ=255)

          cAGTCCCACAACGGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAAt                                                                                   <  1:4706290/100‑1 (MQ=255)

           aGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATGCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATg                                                                                  >  2:3514808/1‑100 (MQ=255)

            gTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGc                                                                                 >  1:4270204/1‑100 (MQ=255)

            gTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGc                                                                                 >  1:4858654/1‑100 (MQ=255)

            gTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGc                                                                                 >  1:500849/1‑100 (MQ=255)

            gTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGc                                                                                 >  2:165637/1‑100 (MQ=255)

            gTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGc                                                                                 >  2:919300/1‑100 (MQ=255)

             tCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGcc                                                                                >  1:2473901/1‑100 (MQ=255)

              cccACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCt                                                                               >  1:5192722/1‑100 (MQ=255)

               ccACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCtt                                                                              >  1:3345283/1‑100 (MQ=255)

               ccACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCtt                                                                              >  2:2289362/1‑100 (MQ=255)

                cacaACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCttt                                                                             >  2:5379710/1‑100 (MQ=255)

                  ctacaGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTg                                                                           <  1:951955/98‑1 (MQ=255)

                  caacaGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTg                                                                           <  2:1387588/100‑1 (MQ=255)

                  caacaGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTg                                                                           <  1:1019124/100‑1 (MQ=255)

                  caacaGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTg                                                                           <  2:4053110/100‑1 (MQ=255)

                   aacaGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGa                                                                          <  2:391769/100‑1 (MQ=255)

                    acaGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGag                                                                         >  2:939340/1‑99 (MQ=255)

                    acaGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGAc                                                                         <  1:2863723/100‑1 (MQ=255)

                    acaGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGAc                                                                         <  1:3166753/100‑1 (MQ=255)

                         cATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTg                                                                    <  2:758759/100‑1 (MQ=255)

                            cGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACa                                                                 <  1:323427/100‑1 (MQ=255)

                            cGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACa                                                                 <  2:3161197/100‑1 (MQ=255)

                              ttAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAgg                                                               >  2:4323215/1‑100 (MQ=255)

                              ttAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAgg                                                               <  1:416985/100‑1 (MQ=255)

                              ttAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAgg                                                               >  1:310646/1‑100 (MQ=255)

                               tAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGt                                                              >  1:903087/1‑100 (MQ=255)

                                  ggCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATg                                                           <  1:1394506/100‑1 (MQ=255)

                                   gCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATgg                                                          <  1:1734320/100‑1 (MQ=255)

                                   gCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATgg                                                          <  1:3594991/100‑1 (MQ=255)

                                     gTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGgtg                                                        >  1:2941987/1‑100 (MQ=255)

                                     gTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGgtg                                                        >  2:1699035/1‑100 (MQ=255)

                                     gTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGgtg                                                        <  2:1601883/100‑1 (MQ=255)

                                     gTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGgtg                                                        >  1:2702495/1‑100 (MQ=255)

                                        gCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTc                                                     >  1:2837244/1‑100 (MQ=255)

                                        gCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTc                                                     >  1:5040902/1‑100 (MQ=255)

                                         cAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCa                                                    >  2:4882152/1‑100 (MQ=255)

                                         cAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCa                                                    <  1:2527368/100‑1 (MQ=255)

                                          aaGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAg                                                   <  1:632516/100‑1 (MQ=255)

                                             gAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGAt                                                >  1:1793815/1‑100 (MQ=255)

                                              aaCGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATc                                               >  1:701037/1‑100 (MQ=255)

                                                cGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGa                                             >  2:4419806/1‑100 (MQ=255)

                                                   tGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGc                                          <  2:4559338/100‑1 (MQ=255)

                                                   tGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGc                                          <  1:3484649/100‑1 (MQ=255)

                                                    gCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCATGATCGATGCg                                         <  1:4959067/100‑1 (MQ=255)

                                                    gCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCg                                         <  2:3883552/100‑1 (MQ=255)

                                                        aCTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATc                                     <  1:1176559/100‑1 (MQ=255)

                                                         ctctGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCg                                    >  1:3949721/1‑100 (MQ=255)

                                                         ctctGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCg                                    >  1:4202656/1‑100 (MQ=255)

                                                          tctGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGa                                   >  1:1079401/1‑100 (MQ=255)

                                                          tctGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGa                                   >  1:1319682/1‑100 (MQ=255)

                                                            tGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACa                                 <  1:3447539/100‑1 (MQ=255)

                                                            tGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACa                                 >  2:4078305/1‑100 (MQ=255)

                                                              cAACTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGc                               >  2:2157716/1‑100 (MQ=255)

                                                              cAACTCGCCTATCCGTGGTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGc                               <  1:5116777/100‑1 (MQ=255)

                                                                ncTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCaa                             >  1:4425031/2‑100 (MQ=255)

                                                                 cTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAAc                            <  2:5208963/100‑1 (MQ=255)

                                                                 cTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAAc                            <  2:2856922/100‑1 (MQ=255)

                                                                 cTCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAAc                            <  2:307727/100‑1 (MQ=255)

                                                                  tCGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACt                           >  1:3738620/1‑100 (MQ=255)

                                                                   cGGCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTc                          >  2:3892332/1‑100 (MQ=255)

                                                                    ggCTATCCGTGCTTCGACTTCCTGCAAGCTTTGCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTcg                         <  2:5500742/100‑1 (MQ=255)

                                                                     gCTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTcgc                        <  1:4832325/100‑1 (MQ=255)

                                                                      cTATCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTcgcg                       <  2:276795/100‑1 (MQ=255)

                                                                        aTCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGa                     <  2:3243641/100‑1 (MQ=255)

                                                                        aTCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGa                     >  1:5458897/1‑100 (MQ=255)

                                                                         tCCGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGAt                    >  2:4631452/1‑100 (MQ=255)

                                                                           cGTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCg                  <  2:4869201/100‑1 (MQ=255)

                                                                            gTGCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGa                 >  1:4012823/1‑100 (MQ=255)

                                                                              gCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGTAACTCGCGGATCGACt               >  1:1467296/1‑100 (MQ=255)

                                                                              gCTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACt               >  1:236621/1‑100 (MQ=255)

                                                                               cTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTc              <  2:1773412/100‑1 (MQ=255)

                                                                               cTTCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTc              >  2:2025027/1‑100 (MQ=255)

                                                                                ttCGACTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTcc             <  2:3269175/100‑1 (MQ=255)

                                                                                   gACTTCCTGCAAGCTTTCATGCACAATCCCTTTTGATTCCTTACAGGCATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCCGACCGACCCCAGa          >  1:5025490/1‑100 (MQ=255)

                                                                                    aCTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGAc         <  2:3420489/100‑1 (MQ=255)

                                                                                     cTTCCTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACt        >  1:3825967/1‑100 (MQ=255)

                                                                                        ccTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACTGaa     <  2:1814398/100‑1 (MQ=255)

                                                                                        ccTGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACTGaa     >  1:4564384/1‑100 (MQ=255)

                                                                                         cTGCAAGCTTTCCTGGACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCACCTCGCGGATCGACTCCAGACTGaaa    >  2:4184894/1‑100 (MQ=255)

                                                                                          tGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACTGAAAc   >  1:744814/1‑100 (MQ=255)

                                                                                          tGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACTGAAAc   >  1:4307506/1‑100 (MQ=255)

                                                                                          tGCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACTGAAAc   <  1:1098072/100‑1 (MQ=255)

                                                                                          tGCAAGCTTTCCTGCACAATGCCTTTTGACTCCAGACAGGTATGGTGTTCAGGATCGATGCGGGTCGACAGCAACTCGCGGATCGACTCCAGACTGAAAc   >  1:5254370/1‑100 (MQ=255)

                                                                                           gCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACTGAAAcc  <  2:2267444/100‑1 (MQ=255)

                                                                                           gCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACTGAAAcc  <  2:1206740/100‑1 (MQ=255)

                                                                                           gCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACTGAAAcc  >  2:3348248/1‑100 (MQ=255)

                                                                                           gCAAGCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACTGAAAcc  >  1:1580507/1‑100 (MQ=255)

CTGCTATGAGCAGTCCCACAACAGGCATCGTTAAGGCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACTGAAACC  >  NC_000913/3438769‑3438959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A