Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,595,235 A→C F206C (TTT→TGT)  lgoR ← putative transcriptional activator for L‑galactonate catabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,595,2350AC97.2% 722.7 / 5.8 211F206C (TTT→TGT) lgoRputative transcriptional activator for L‑galactonate catabolism
Reads supporting (aligned to +/- strand):  ref base A (4/2);  new base C (118/87);  total (122/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 211 total aligned reads displayed.

ACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGT  >  NC_000913/4595139‑4595331
                                                                                                |                                                                                                
 cTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCt                                                                                              >  2:1753598/1‑100 (MQ=255)
   gagaTGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTAt                                                                                            <  2:3737433/100‑1 (MQ=255)
   gagaTGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTAt                                                                                            >  2:2123864/1‑100 (MQ=255)
    agaTGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTAtt                                                                                           <  2:2388425/100‑1 (MQ=255)
     gatgatCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTg                                                                                          >  2:4865385/1‑100 (MQ=255)
       tgatCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGcc                                                                                        >  2:841381/1‑100 (MQ=255)
       tgatCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGcc                                                                                        >  2:1594042/1‑100 (MQ=255)
       tgatCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGcc                                                                                        >  2:3769939/1‑100 (MQ=255)
         atCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGa                                                                                      >  1:2720214/1‑100 (MQ=255)
          tctcAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAt                                                                                     >  2:2941769/1‑100 (MQ=255)
          tctcAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAt                                                                                     <  2:2720827/100‑1 (MQ=255)
          tctcAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAt                                                                                     >  1:243238/1‑100 (MQ=255)
           ctcAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtg                                                                                    <  1:4855373/100‑1 (MQ=255)
            tcAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtgt                                                                                   >  1:3464010/1‑100 (MQ=255)
            tcAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGAtgt                                                                                   >  2:3185347/1‑100 (MQ=255)
             cAAGCGATTGATCAAAAAAGATATTGTCTGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtgtt                                                                                  <  1:3668045/100‑1 (MQ=255)
             cAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtgtt                                                                                  >  2:3615240/1‑100 (MQ=255)
              aaGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtgttg                                                                                 <  1:180925/100‑1 (MQ=255)
              aaGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtgttg                                                                                 >  2:899767/1‑100 (MQ=255)
               aGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtgttgt                                                                                <  2:1900150/100‑1 (MQ=255)
               aGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtgttgt                                                                                <  1:884416/100‑1 (MQ=255)
                 cGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCa                                                                              <  2:5292041/100‑1 (MQ=255)
                  gATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACGTGCGACAGCTATTGCCGATGTTGTCAc                                                                             <  1:5046678/100‑1 (MQ=255)
                     tGATCAAAAAAGATATCGGCGGCAGCTCAAAGCAGTAAGGAGTGAAAATCTCTGTAAAGTTGCGACAACACGCGACCCCTCTTTCCCACGTTGTCACGCa                                                                          >  2:4350373/1‑100 (MQ=255)
                      gATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAg                                                                         >  1:2619721/1‑100 (MQ=255)
                      gATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAg                                                                         <  1:1443258/100‑1 (MQ=255)
                       aTCAAATAAGATATTGTCGGCAGCTGCAAGCCGTAAGGCGTGAAAATCTCTGACAAGTTGCGAGAACCCGCGACAGCTTTTGCCGATGTTGTCACGCAGt                                                                        >  2:1319577/1‑100 (MQ=255)
                       aTCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGt                                                                        >  1:1316914/1‑100 (MQ=255)
                         cAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAg                                                                      >  2:1531978/1‑100 (MQ=255)
                         cAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAg                                                                      >  1:2404102/1‑100 (MQ=255)
                          aaaaaaGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGc                                                                     >  1:3848628/1‑100 (MQ=255)
                           aaaaaGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGCTGTTGTCACGCAGTAGcc                                                                    >  1:5250092/1‑100 (MQ=255)
                              aaGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGAt                                                                 <  2:456146/100‑1 (MQ=255)
                              aaGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGAt                                                                 <  1:3029713/100‑1 (MQ=255)
                               aGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATg                                                                <  1:5340958/100‑1 (MQ=255)
                                gATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGa                                                               <  2:485072/100‑1 (MQ=255)
                                gATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGa                                                               <  1:3785264/100‑1 (MQ=255)
                                gATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGa                                                               >  1:2936646/1‑100 (MQ=255)
                                 atatTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGAc                                                              <  2:1857902/100‑1 (MQ=255)
                                  tatTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACg                                                             <  1:2379698/100‑1 (MQ=255)
                                     tGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTc                                                          >  1:9108/1‑100 (MQ=255)
                                     tGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTc                                                          <  1:4696387/100‑1 (MQ=255)
                                      gTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCg                                                         >  1:2991316/1‑100 (MQ=255)
                                      gTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCg                                                         >  2:4141075/1‑100 (MQ=255)
                                         ggCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGc                                                      >  1:1006647/1‑100 (MQ=255)
                                            aGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATg                                                   <  2:3250897/100‑1 (MQ=255)
                                             gCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATgg                                                  >  1:5028666/1‑100 (MQ=255)
                                             gCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATgg                                                  <  1:4963593/100‑1 (MQ=255)
                                             gCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATgg                                                  >  1:5093673/1‑100 (MQ=255)
                                              cTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGt                                                 >  1:3443062/1‑100 (MQ=255)
                                               tGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGtt                                                >  2:4017593/1‑100 (MQ=255)
                                                gAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGttt                                               >  2:1803910/1‑100 (MQ=255)
                                                gAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGttt                                               <  2:815199/100‑1 (MQ=255)
                                                gAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGttt                                               >  1:5170570/1‑100 (MQ=255)
                                                gAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGttt                                               >  1:3403947/1‑100 (MQ=255)
                                                     cAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGATAACATGCGAAAGCTATTGCCGATGTTTTCACTCATGATCCGATGACGTTCGAGCATGGTGTTCGcc                                          >  2:1362679/1‑100 (MQ=255)
                                                     cAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGcc                                          <  2:3350142/100‑1 (MQ=255)
                                                     cAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGcc                                          >  2:1257244/1‑100 (MQ=255)
                                                     cAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGcc                                          <  2:3459138/100‑1 (MQ=255)
                                                      aGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCt                                         <  1:133745/100‑1 (MQ=255)
                                                       gTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTTGCCGATGACGTTCGAGCATGGTTTTCGCCTg                                        <  1:4459995/100‑1 (MQ=255)
                                                       gTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTg                                        >  1:4061078/1‑100 (MQ=255)
                                                       gTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTg                                        >  2:2680846/1‑100 (MQ=255)
                                                       ctAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTg                                        <  1:3581144/99‑1 (MQ=255)
                                                         aaGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCAGGCAGAAGCCGATGACGTTCGAGCATGTTTTTCGcgcgca                                      >  2:4516516/1‑95 (MQ=255)
                                                         aaGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTgag                                      >  2:3548049/1‑98 (MQ=255)
                                                         aaGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGca                                      >  1:1759590/1‑100 (MQ=255)
                                                          aGGAGTGAAAATCACTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGACGATGACGTTCGAGCATGGTTTTCGCCTGcag                                     >  2:2475265/1‑100 (MQ=255)
                                                            gAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcagcc                                   >  1:1514494/1‑100 (MQ=255)
                                                            gAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcagcc                                   <  1:666042/100‑1 (MQ=255)
                                                             aGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcagcca                                  >  1:1733952/1‑100 (MQ=255)
                                                              gTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcagccag                                 >  2:1154066/1‑100 (MQ=255)
                                                              gTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcagccag                                 >  1:951273/1‑100 (MQ=255)
                                                              gTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcagccag                                 >  2:2260897/1‑100 (MQ=255)
                                                               tGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcagccagc                                >  1:623192/1‑100 (MQ=255)
                                                                gAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACCGTCGTGCATGGTTTTCGCCTGCAGCCAGCg                               >  2:5213217/1‑100 (MQ=255)
                                                                 aaaaTCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCgg                              >  2:357871/1‑100 (MQ=255)
                                                                 aaaaTCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCgg                              <  1:41449/100‑1 (MQ=255)
                                                                    aTCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGa                           <  1:1824485/100‑1 (MQ=255)
                                                                     tctcTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGAt                          <  1:2223487/100‑1 (MQ=255)
                                                                      ctctGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATc                         <  2:722263/100‑1 (MQ=255)
                                                                       tctGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCa                        <  2:5127644/100‑1 (MQ=255)
                                                                            cAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATc                   <  1:4629387/100‑1 (MQ=255)
                                                                            cAAGTTGCCAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCTAGCATGGTTTTCTCCTGCAGCCAGAGGGGATCATGAtg                   >  2:4587657/1‑99 (MQ=255)
                                                                             aaGTTGCGAGAACATGCGACAGCTATTGCCGATGTTTTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATca                  <  2:2969625/100‑1 (MQ=255)
                                                                             aaGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATca                  <  1:5459681/100‑1 (MQ=255)
                                                                             aaGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATca                  <  1:4382393/100‑1 (MQ=255)
                                                                              aGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATcag                 <  1:3529263/100‑1 (MQ=255)
                                                                               gTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATcagg                >  1:2691522/1‑100 (MQ=255)
                                                                               gTTGCGAGAACATGCGACAGCTATTGCCGATGATGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATcagg                <  2:5498942/100‑1 (MQ=255)
                                                                               gTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATcagg                <  1:3215031/100‑1 (MQ=255)
                                                                                    gagaACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGt           <  2:4078565/100‑1 (MQ=255)
                                                                                      gaACATGCGACCGCTATTGCCGATCTTGTCACGCAGCAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTg         <  1:253957/100‑1 (MQ=255)
                                                                                      gaACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTg         <  1:2820643/100‑1 (MQ=255)
                                                                                      gaACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTg         >  2:955340/1‑100 (MQ=255)
                                                                                       aaCATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTga        <  1:3719019/100‑1 (MQ=255)
                                                                                        aCATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTgag       >  2:4993817/1‑100 (MQ=255)
                                                                                          aTGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGaa     <  1:1612892/100‑1 (MQ=255)
                                                                                           tGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGaaa    >  2:3660787/1‑100 (MQ=255)
                                                                                           tGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGaaa    >  1:2090766/1‑100 (MQ=255)
                                                                                                |                                                                                                
ACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGT  >  NC_000913/4595139‑4595331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A