Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,595,235 A→C F206C (TTT→TGT)  lgoR ← putative transcriptional activator for L‑galactonate catabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,595,2350AC99.4% 587.0 / ‑4.4 168F206C (TTT→TGT) lgoRputative transcriptional activator for L‑galactonate catabolism
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (72/94);  total (74/94)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 168 total aligned reads displayed.

GACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAG  >  NC_000913/4595138‑4595330
                                                                                                 |                                                                                               
gACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAg                                                                                               <  1:4027927/100‑1 (MQ=255)
 aCTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGc                                                                                              <  2:3048183/100‑1 (MQ=255)
 aCTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGc                                                                                              >  2:3159522/1‑100 (MQ=255)
 aCTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGAAGTAAGGAATGAAAATCTCTGTCAAGTTGTGAGAAAATGCGACAGc                                                                                              >  2:521359/1‑100 (MQ=255)
  cTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCt                                                                                             >  2:1516453/1‑100 (MQ=255)
  cTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCt                                                                                             >  2:2229374/1‑100 (MQ=255)
  cTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCt                                                                                             >  2:1573508/1‑100 (MQ=255)
  cTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCt                                                                                             >  1:409410/1‑100 (MQ=255)
    nagaTGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTAt                                                                                           >  1:1505747/2‑100 (MQ=255)
    gagaTGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTAt                                                                                           <  1:1727306/100‑1 (MQ=255)
      gatgatCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTg                                                                                         <  2:3662622/100‑1 (MQ=255)
      gatgatCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTg                                                                                         <  2:299786/100‑1 (MQ=255)
        tgatCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGcc                                                                                       >  1:2864318/1‑100 (MQ=255)
        tgatCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGcc                                                                                       <  2:1818231/100‑1 (MQ=255)
        tgatCTCAAGCGATTGAGAAACAAATATATGGTCGGTAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGcc                                                                                       <  2:3786038/100‑1 (MQ=255)
         gatCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCg                                                                                      >  1:2919200/1‑100 (MQ=255)
          atCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGa                                                                                     >  1:3837516/1‑100 (MQ=255)
          atCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGa                                                                                     >  1:3838364/1‑100 (MQ=255)
            ctcAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtg                                                                                   <  1:3972653/100‑1 (MQ=255)
             tcAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtgt                                                                                  <  2:10282/100‑1 (MQ=255)
              cAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtgtt                                                                                 <  1:2409648/100‑1 (MQ=255)
              cAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtgtt                                                                                 >  2:2113077/1‑100 (MQ=255)
              cAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtgtt                                                                                 <  1:88950/100‑1 (MQ=255)
                aGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGAtgttgt                                                                               >  2:2538093/1‑100 (MQ=255)
                 gCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTc                                                                              <  1:114601/100‑1 (MQ=255)
                 gCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTc                                                                              >  2:162594/1‑100 (MQ=255)
                  cGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCa                                                                             <  1:1017855/100‑1 (MQ=255)
                  cGATTGATCAAAAAAGATATTGACGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCa                                                                             <  2:3218620/100‑1 (MQ=255)
                   gATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCAc                                                                            >  1:83406/1‑100 (MQ=255)
                    aTTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACg                                                                           <  2:1717418/100‑1 (MQ=255)
                        aTCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGTGACAGCTATTGCCGATGTTGTCACGCAGt                                                                       >  2:1263057/1‑100 (MQ=255)
                        aTCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGt                                                                       >  1:1568065/1‑100 (MQ=255)
                        aTCAAAAAAGAAATTGTCGGCAGCTGAAAGCAGTACGGAGTGCAAGTCCCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGATGTCACGCAGt                                                                       >  2:3205067/1‑100 (MQ=255)
                          cAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAg                                                                     >  2:2834650/1‑100 (MQ=255)
                           aaaaaaGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGc                                                                    >  1:2026397/1‑100 (MQ=255)
                           aaaaaaGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGc                                                                    >  2:2872576/1‑100 (MQ=255)
                           aaaaaaGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGc                                                                    <  1:2383833/100‑1 (MQ=255)
                            aaaaaGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGcc                                                                   <  1:1396875/100‑1 (MQ=255)
                               aaGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGAt                                                                <  1:3097426/100‑1 (MQ=255)
                                aGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATg                                                               <  1:3907070/100‑1 (MQ=255)
                                 cgatctGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGa                                                              <  1:2601226/95‑1 (MQ=255)
                                       gTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCg                                                        <  1:1989409/100‑1 (MQ=255)
                                         cGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAg                                                      <  2:942658/100‑1 (MQ=255)
                                         cGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAg                                                      >  1:693473/1‑100 (MQ=255)
                                          ggCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGc                                                     >  1:2988066/1‑100 (MQ=255)
                                          ggCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGc                                                     >  1:696195/1‑100 (MQ=255)
                                             aGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATg                                                  <  1:27423/100‑1 (MQ=255)
                                             aGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATg                                                  <  1:1600480/100‑1 (MQ=255)
                                              gCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATgg                                                 >  1:2287986/1‑100 (MQ=255)
                                              gCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATgg                                                 >  1:1740063/1‑100 (MQ=255)
                                              gCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATgg                                                 >  2:803897/1‑100 (MQ=255)
                                              gCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATgg                                                 >  2:1159250/1‑100 (MQ=255)
                                               cTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGt                                                >  1:3451563/1‑100 (MQ=255)
                                               cTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGt                                                >  1:3663001/1‑100 (MQ=255)
                                                tGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGtt                                               >  1:3429374/1‑100 (MQ=255)
                                                tGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGtt                                               >  1:2699783/1‑100 (MQ=255)
                                                 gAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGttt                                              <  1:3470246/100‑1 (MQ=255)
                                                 gAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGttt                                              >  1:2636620/1‑100 (MQ=255)
                                                      cAGTCAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGCTGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGcc                                         <  1:1296858/100‑1 (MQ=255)
                                                      cAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGcc                                         <  1:3307717/100‑1 (MQ=255)
                                                      cAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGcc                                         <  1:1467410/100‑1 (MQ=255)
                                                      cAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGcc                                         <  1:1635833/100‑1 (MQ=255)
                                                        gTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTg                                       <  2:2900586/100‑1 (MQ=255)
                                                        gTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTg                                       >  1:4071111/1‑100 (MQ=255)
                                                        gTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTg                                       <  2:3065711/100‑1 (MQ=255)
                                                           aGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcag                                    <  1:2086679/100‑1 (MQ=255)
                                                            ggAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcagc                                   <  1:2248661/100‑1 (MQ=255)
                                                               gTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcagccag                                >  1:3685696/1‑100 (MQ=255)
                                                               gTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcagccag                                <  2:1294018/100‑1 (MQ=255)
                                                               gTGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGcagccag                                >  1:3330437/1‑100 (MQ=255)
                                                                tGAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTATCACGAAGTAGCCGATGACGTTCGAGAATGGTTTTCGCCTGcagccagc                               >  1:2878314/1‑100 (MQ=255)
                                                                 gAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGCGCATGGTTTTCGCCTGCCGCCAGCg                              >  1:1938893/1‑100 (MQ=255)
                                                                 gAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGTAGCCAGCg                              <  2:3833860/100‑1 (MQ=255)
                                                                 gAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCg                              >  2:1365777/1‑100 (MQ=255)
                                                                 gAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCg                              >  2:1100924/1‑100 (MQ=255)
                                                                 gAAAATCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCg                              >  2:271987/1‑100 (MQ=255)
                                                                  aaaaTCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCTAGCATGGTTTTCGCCTGCAGCCAGCgg                             <  2:437086/100‑1 (MQ=255)
                                                                   aaaTCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCggg                            <  1:276549/100‑1 (MQ=255)
                                                                     aTCTCTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGa                          <  1:801893/100‑1 (MQ=255)
                                                                      tctcTGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGAt                         <  2:280123/100‑1 (MQ=255)
                                                                       ctctGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATc                        <  2:3971046/100‑1 (MQ=255)
                                                                       ctctGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATc                        <  1:2461752/100‑1 (MQ=255)
                                                                        tctGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCa                       <  2:3925994/100‑1 (MQ=255)
                                                                          tGTCAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATg                     <  1:3766005/100‑1 (MQ=255)
                                                                             cAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATc                  <  2:2461680/100‑1 (MQ=255)
                                                                             cAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATc                  >  2:2420312/1‑100 (MQ=255)
                                                                             cAAGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATc                  <  2:592276/100‑1 (MQ=255)
                                                                              aaGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATca                 <  1:821159/100‑1 (MQ=255)
                                                                              aaGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATca                 <  2:1789992/100‑1 (MQ=255)
                                                                              aaGTTGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATca                 >  2:1391484/1‑100 (MQ=255)
                                                                                  tGCGAGAACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATcaggca             >  2:1238749/1‑100 (MQ=255)
                                                                                     gagaACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGt          <  1:110330/100‑1 (MQ=255)
                                                                                     gagaACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGt          <  2:2802614/100‑1 (MQ=255)
                                                                                     gagaACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGt          <  1:3188495/100‑1 (MQ=255)
                                                                                     gagaACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGt          >  1:2845521/1‑100 (MQ=255)
                                                                                     gagaACATGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGt          <  2:1858458/100‑1 (MQ=255)
                                                                                       gaACATGCGACAGCGATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTg        <  2:618917/100‑1 (MQ=255)
                                                                                           aTGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGaa    <  1:1682526/100‑1 (MQ=255)
                                                                                           aTGCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGaa    <  2:2629046/100‑1 (MQ=255)
                                                                                             gCGACAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAg  <  1:1181893/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GACTGAGATGATCTCAAGCGATTGATCAAAAAAGATATTGTCGGCAGCTGAAAGCAGTAAGGAGTGAAAATCTCTGTCAAGTTGCGAGAACATGCGAAAGCTATTGCCGATGTTGTCACGCAGTAGCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAG  >  NC_000913/4595138‑4595330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A