breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 6,809 T→G K384T (AAA→ACA)  yaaJ ← putative transporter
RA 14,011 T→G S617A (TCT→GCT)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ
RA 25,474 T→G S90A (TCG→GCG)  lspA → prolipoprotein signal peptidase (signal peptidase II)
RA 43,187 T→G intergenic (+14/‑1) fixA → / → fixB anaerobic carnitine reduction putative electron transfer flavoprotein subunit/putative electron transfer flavoprotein, NAD/FAD‑binding domain and ETFP adenine nucleotide‑binding domain‑like protein
RA 75,116 T→G Y122S (TAT→TCT)  thiB ← thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
RA 76,539 T→G E254A (GAA→GCA)  sgrR ← transcriptional DNA‑binding transcriptional activator of sgrS sRNA
RA 95,418 T→G S257A (TCG→GCG)  murF → UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
JC JC 111,051 IS1 (–) +9 bp coding (8‑16/390 nt) mutT → dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
RA 122,070 A→C intergenic (‑519/‑22) aroP ← / → pdhR aromatic amino acid transporter/pyruvate dehydrogenase complex repressor; autorepressor
RA 142,754 A→C intergenic (‑84/‑25) can ← / → yadG carbonic anhydrase/putative ABC transporter ATPase
RA 143,263 A→C E162A (GAA→GCA)  yadG → putative ABC transporter ATPase
RA 162,349 A→C Y82S (TAC→TCC)  hrpB → putative ATP‑dependent helicase
RA 175,540 T→G L145W (TTG→TGG)  clcA → H(+)/Cl(‑) exchange transporter
RA 199,001 T→G D358E (GAT→GAG bamA → BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
RA 206,867 A→C E581A (GAG→GCG)  dnaE → DNA polymerase III alpha subunit
RA 218,130 T→G S216R (AGC→CGC)  proS ← prolyl‑tRNA synthetase
RA 299,214 A→C F574C (TTC→TGC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit
RA 301,463 T→G E142A (GAA→GCA)  paoB ← PaoABC aldehyde oxidoreductase, FAD‑containing subunit
RA 313,275 T→G intergenic (‑33/+441) ykgR ← / ← ykgP uncharacterized protein/pseudogene, oxidoreductase family
RA 344,915 T→G I247R (ATA→AGA)  yahL → uncharacterized protein
RA 388,155 T→G S121A (TCG→GCG)  tauD → taurine dioxygenase, 2‑oxoglutarate‑dependent
RA 394,041 T→G pseudogene (1072/1449 nt) yaiT → pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
RA 400,871 T→G E18A (GAA→GCA)  ddlA ← D‑alanine‑D‑alanine ligase A
RA 424,279 A→C T588P (ACT→CCT)  malZ → maltodextrin glucosidase
RA 428,252 T→G I202M (ATT→ATG secD → SecYEG protein translocase auxillary subunit
RA 431,340 A→C I225S (ATC→AGC)  tsx ← nucleoside channel, receptor of phage T6 and colicin K
RA 480,566 A→C L48W (TTG→TGG)  tomB ← Hha toxicity attenuator; conjugation‑related protein
RA 488,082 T→G I516S (ATT→AGT)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated
RA 513,767 A→C S398R (AGT→CGT)  ybaT → putative amino acid transporter
RA 546,173 T→G E64A (GAA→GCA)  allD ← ureidoglycolate dehydrogenase
RA 547,728 T→G L350R (CTT→CGT)  fdrA → putative NAD(P)‑binding acyl‑CoA synthetase
RA 576,137 T→G pseudogene (689/1040 nt) nmpC ← DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage
RA 611,121 A→C S458R (AGT→AGG fepA ← ferrienterobactin outer membrane transporter
RA 615,736 A→C E527A (GAA→GCA)  entF → enterobactin synthase multienzyme complex component, ATP‑dependent
RA 619,006 A→C G251G (GGA→GGC fepE → regulator of length of O‑antigen component of lipopolysaccharide chains
RA 621,281 A→C I303M (ATT→ATG fepD ← ferrienterobactin ABC transporter permease
RA 623,458 T→G L387V (TTG→GTG)  entS → enterobactin exporter, iron‑regulated
RA 716,235 A→C pseudogene (363/651 nt) ybfG ← pseudogene
RA 741,597 T→G A320A (GCA→GCC dtpD ← dipeptide and tripeptide permease D
RA 754,738 A→C intergenic (‑270/‑439) gltA ← / → sdhC citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556
RA 783,757 A→C I198L (ATT→CTT)  pnuC → nicotinamide riboside transporter
RA 797,813 A→C W207G (TGG→GGG)  ybhA ← pyridoxal phosphate (PLP) phosphatase
RA 825,545 T→G V188V (GTA→GTC ybhR ← putative ABC transporter permease
RA 832,388 T→G intergenic (+152/+80) rhlE → / ← ybiA ATP‑dependent RNA helicase/DUF1768 family protein
RA 846,198 A→C F89C (TTC→TGC)  glnQ ← glutamine transporter subunit
RA 885,494 A→C F213C (TTT→TGT)  ybjI ← 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase; pyrimidine phosphatase; riboflavin synthesis
RA 908,807 A→C L163R (CTG→CGG)  ltaE ← L‑allo‑threonine aldolase, PLP‑dependent
RA 917,078 T→G D202E (GAT→GAG ybjD → putative OLD family ATP‑dependent endonuclease; DUF2813 family protein
RA 921,236 A→C K297T (AAA→ACA)  macB → macrolide ABC transporter peremase/ATPase
RA 930,957 A→C N2K (AAT→AAG cydD ← glutathione/cysteine ABC transporter export permease/ATPase
RA 960,657 C→T L132L (CTG→TTG)  ycaL → putative peptidase‑related chaperone
RA 969,341 T→G S326A (TCT→GCT)  lpxK → lipid A 4'kinase
RA 970,734 A→C N21T (AAC→ACC)  ycaR → peroxide and acid resistance protein, UPF0434 family
RA 1,035,960 T→G L64R (CTC→CGC)  hyaD → hydrogenase 1 maturation protease
RA 1,036,544 A→C E64A (GAG→GCG)  hyaE → putative HyaA chaperone
RA 1,048,134 A→C N186K (AAT→AAG gfcC ← putative O‑antigen capsule production periplasmic protein
RA 1,064,004 T→G intergenic (‑229/‑32) cbpA ← / → yccE DnaK co‑chaperone; curved DNA‑binding protein/uncharacterized protein
RA 1,078,882 T→G M1M (ATG→CTG) † putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
RA 1,093,771 A→C E299A (GAA→GCA)  ycdT → diguanylate cyclase, membrane‑anchored
RA 1,134,118 A→C Q106P (CAG→CCG)  flgF → flagellar component of cell‑proximal portion of basal‑body rod
RA 1,139,916 T→G D513E (GAT→GAG flgK → flagellar hook‑filament junction protein 1
RA 1,156,452 T→G S231A (TCG→GCG)  holB → DNA polymerase III, delta prime subunit
RA 1,183,048 T→G L191F (TTA→TTC potC ← spermidine/putrescine ABC transporter permease
RA 1,224,314 T→G N78H (AAC→CAC)  minE ← cell division topological specificity factor
RA 1,245,298 T→G S47A (TCG→GCG)  ycgY → uncharacterized protein
RA 1,290,933 T→G L231R (CTT→CGT)  rssB → PcnB‑degradosome interaction factor; response regulator
RA 1,294,845 A→C pseudogene (148/567 nt) insZ ← pseudogene, transposase homolog
RA 1,311,596 T→G L170V (TTG→GTG)  tonB → membrane spanning protein in TonB‑ExbB‑ExbD transport complex
RA 1,335,881 T→G D17E (GAT→GAG acnA → aconitate hydratase 1; aconitase A
RA 1,343,516 A→C intergenic (‑188/+81) osmB ← / ← yciT osmotically and stress inducible lipoprotein/global regulator of transcription; DeoR family
RA 1,438,947 T→G E613A (GAA→GCA)  pfo ← pyruvate‑flavodoxin oxidoreductase
RA 1,479,936 A→C N267H (AAC→CAC)  ynbC → putative esterase
RA 1,494,054 T→G S529A (TCT→GCT)  trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
RA 1,494,520 A→C N184K (AAT→AAG ydcI ← putative DNA‑binding transcriptional regulator
RA 1,571,816 T→G D77A (GAT→GCT)  gadB ← glutamate decarboxylase B, PLP‑dependent
RA 1,597,634 A→C pseudogene (354/3861 nt) yneO ← pseudogene, AidA homolog
RA 1,617,428 A→C K134T (AAG→ACG)  ydeA → arabinose efflux transporter, arabinose‑inducible
RA 1,619,743 A→C Y57S (TAC→TCC)  marA → multiple antibiotic resistance transcriptional regulator
RA 1,638,733 A→C intergenic (‑66/+297) cspI ← / ← rzpQ Qin prophage; cold shock protein/Rz‑like protein, Qin prophage
RA 1,647,986 A→C N18T (AAC→ACC)  dicA → Qin prophage; putative regulator for DicB
RA 1,660,346 A→C M760L (ATG→CTG)  ynfE → putative selenate reductase, periplasmic
RA 1,681,460 T→G M162R (ATG→AGG)  folM → dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme
RA 1,714,643 T→G I89M (ATT→ATG gstA → glutathionine S‑transferase
RA 1,716,402 A→C F274C (TTC→TGC)  tyrS ← tyrosyl‑tRNA synthetase
RA 1,756,929 T→G L411R (CTG→CGG)  pykF → pyruvate kinase I
RA 1,815,451 T→G F529V (TTT→GTT)  katE → catalase HPII, heme d‑containing
RA 1,822,043 T→G I72L (ATC→CTC)  osmE ← osmotically‑inducible lipoprotein
RA 1,823,297 T→G intergenic (+12/‑218) nadE → / → cho NAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair
RA 1,830,953 T→G N344H (AAT→CAT)  astC ← succinylornithine transaminase, PLP‑dependent
RA 1,896,050 A→C N416H (AAC→CAC)  pabB → aminodeoxychorismate synthase, subunit I
RA 1,921,059 T→G P279P (CCT→CCG rsmF → 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 1,985,983 T→G K49T (AAA→ACA)  araF ← L‑arabinose ABC transporter periplasmic binding protein
RA 1,987,532 A→C F76C (TTT→TGT)  yecJ ← DUF2766 family protein
RA 2,008,526 A→C I84L (ATC→CTC)  yedE → UPF0394 family sulphur transport domain‑containing inner membrane protein
RA 2,019,670 T→G I18S (ATT→AGT)  fliL → flagellar biosynthesis protein
RA 2,024,185 A→C N73T (AAT→ACT)  rcsA → transcriptional regulator of colanic acid capsular biosynthesis
RA 2,036,444 T→G Y204D (TAT→GAT)  hchA → glyoxalase III and Hsp31 molecular chaperone
RA 2,075,512 T→G L245R (CTG→CGG)  yeeR → CP4‑44 prophage; putative membrane protein
RA 2,113,790 T→G K180Q (AAA→CAA)  wcaN ← putative regulatory subunit for GalU
RA 2,127,751 T→G E188D (GAA→GAC gmd ← GDP‑D‑mannose dehydratase, NAD(P)‑binding
RA 2,151,093 T→G intergenic (‑108/‑92) yegI ← / → yegJ protein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein
RA 2,152,171 T→G E101A (GAA→GCA)  yegK ← ser/thr phosphatase‑related protein
RA 2,154,851 T→G L279R (CTA→CGA)  mdtA → multidrug efflux system, subunit A
RA 2,164,104 A→C Y410S (TAT→TCT)  baeS → sensory histidine kinase in two‑component regulatory system with BaeR
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,181,901 A→C D269A (GAC→GCC)  yegV → putative kinase
RA 2,221,860 T→G K44Q (AAA→CAA)  bglX ← beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,281,112 A→C I161L (ATC→CTC)  yejH → putative ATP‑dependent DNA or RNA helicase
RA 2,292,894 A→C L24R (CTG→CGG)  dsbE ← periplasmic thioredoxin of cytochrome c‑type biogenesis
RA 2,318,639 A→C F413C (TTT→TGT)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 2,323,353 T→G intergenic (+102/‑94) atoC → / → atoD fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
RA 2,361,733 A→C L160R (CTG→CGG)  rhmR ← putative DNA‑binding transcriptional regulator for the rhm operon
RA 2,373,622 A→C intergenic (‑84/+26) pmrD ← / ← menE inactive two‑component system connector protein/O‑succinylbenzoate‑CoA ligase
RA 2,387,305 G→A P124P (CCG→CCA yfbM → DUF1877 family protein
RA 2,397,296 A→C P49P (CCT→CCG nuoH ← NADH:ubiquinone oxidoreductase, membrane subunit H
RA 2,411,252 A→C I34S (ATT→AGT)  yfbS ← putative transporter
RA 2,423,570 A→C intergenic (‑31/+166) yfcI ← / ← hisP transposase_31 family protein/histidine ABC transporter ATPase
RA 2,424,497 T→G K5Q (AAA→CAA)  hisP ← histidine ABC transporter ATPase
RA 2,483,809 T→G L19V (TTA→GTA)  evgA → response regulator in two‑component regulatory system with EvgS
RA 2,488,822 A→C L116R (CTT→CGT)  yfdE ← acetyl‑CoA:oxalate CoA‑transferase
RA 2,497,996 T→G E100D (GAA→GAC alaC ← glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase
RA 2,502,542 T→G Q648H (CAA→CAC fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components
RA 2,518,687 A→C E74A (GAA→GCA)  yfeC → DUF1323 family putative DNA‑binding protein
RA 2,616,067 T→G intergenic (‑186/‑27) yfgO ← / → bepA putative UPF0118 family inner membrane permease/OM protein maintenance and assembly metalloprotease and chaperone, periplasmic
RA 2,704,845 T→G K155N (AAA→AAC lepB ← leader peptidase (signal peptidase I)
RA 2,717,422 A→C V223V (GTA→GTC ung → uracil‑DNA‑glycosylase
RA 2,772,145 T→G pseudogene (10/153 nt) yfjU ← CP4‑57 prophage; conserved protein;Phage or Prophage Related
RA 2,854,496 A→C Q53H (CAA→CAC fhlA → formate hydrogenlyase transcriptional activator
RA 2,869,491 A→C L10R (CTG→CGG)  pcm ← L‑isoaspartate protein carboxylmethyltransferase type II
RA 2,899,375 A→C I15S (ATT→AGT)  ygcU ← putative FAD‑linked oxidoreductase
RA 2,904,306 T→G intergenic (+932/+441) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA 2,922,239 T→G intergenic (‑139/+296) gudP ← / ← yqcA putative D‑glucarate transporter/short‑chain flavodoxin, FMN‑binding
RA 2,934,721 T→G S163A (TCG→GCG)  fucP → L‑fucose transporter
RA 2,957,342 T→G K515Q (AAG→CAG)  ptrA ← protease III
RA 2,966,116 A→C intergenic (‑79/+72) lgt ← / ← ptsP phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase/PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
RA 2,968,180 T→G I85I (ATA→ATC ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
RA 2,988,621 T→G L40L (CTT→CTG yqeI → putative transcriptional regulator
RA 3,007,340 A→C E360A (GAA→GCA)  ygeW → putative carbamoyltransferase
RA 3,009,624 A→C K287N (AAA→AAC ygeY → putative peptidase
RA 3,014,506 T→G Q184H (CAA→CAC yqeC ← putative selenium‑dependent hydroxylase accessory protein
RA 3,026,011 T→G H82Q (CAT→CAG guaD → guanine deaminase
RA 3,040,512 A→C G104G (GGT→GGG cptA ← toxin of CptAB toxin‑antitoxin pair
RA 3,044,628 A→C K323Q (AAG→CAG)  bglA → 6‑phospho‑beta‑glucosidase A
RA 3,056,034 A→C noncoding (52/183 nt) ssrS → 6S RNA inhibits RNA polymerase promoter binding
RA 3,082,025 A→C Y38S (TAC→TCC)  loiP → Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; Era‑binding heat shock protein
RA 3,089,170 T→G V296G (GTC→GGC)  galP → D‑galactose transporter
RA 3,095,466 T→G L123L (CTT→CTG yggS → UPF0001 family protein, PLP‑binding
RA 3,101,594 T→G A10A (GCA→GCC yggN ← DUF2884 family putative periplasmic protein
RA 3,105,513 T→G intergenic (+1/‑201) mltC → / → nupG membrane‑bound lytic murein transglycosylase C/nucleoside transporter
RA 3,108,760 A→C H132Q (CAT→CAG speC ← ornithine decarboxylase, constitutive
RA 3,151,112 A→C D44E (GAT→GAG exbD ← membrane spanning protein in TonB‑ExbB‑ExbD complex
RA 3,174,035 T→G E454D (GAA→GAC parE ← DNA topoisomerase IV, subunit B
RA 3,174,612 A→C L262R (CTG→CGG)  parE ← DNA topoisomerase IV, subunit B
RA 3,180,239 A→C K166Q (AAA→CAA)  ygiB → DUF1190 family protein
RA 3,182,529 A→C intergenic (‑122/‑21) ygiD ← / → zupT 4,5‑ DOPA‑extradiol‑dioxygenase/zinc transporter
RA 3,197,496 T→G T716P (ACT→CCT)  glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
RA 3,198,644 A→C F333C (TTC→TGC)  glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
RA 3,207,921 A→C E184A (GAA→GCA)  ttdB → L‑tartrate dehydratase, beta subunit
RA 3,271,880 T→G E291A (GAA→GCA)  garR ← tartronate semialdehyde reductase
RA 3,290,794 A→C I661L (ATC→CTC)  yraJ → putative outer membrane protein
RA 3,303,518 A→C E24A (GAA→GCA)  yhbW → putative luciferase‑like monooxygenase
RA 3,334,314 A→C Y202S (TAT→TCT)  ispB → octaprenyl diphosphate synthase
RA 3,363,167 T→G L121V (TTA→GTA)  yhcD → putative outer membrane fimbrial subunit usher protein
RA 3,387,576 T→G K205Q (AAA→CAA)  aaeB ← p‑hydroxybenzoic acid efflux system component
RA 3,397,618 T→G I60L (ATT→CTT)  rng ← ribonuclease G
RA 3,399,779 A→C L67R (CTG→CGG)  mreC ← cell wall structural complex MreBCD transmembrane component MreC
RA 3,444,566 A→C F53V (TTC→GTC)  rpmD ← 50S ribosomal subunit protein L30
RA 3,461,243 A→C E74A (GAA→GCA)  gspG → pseudopilin, cryptic, general secretion pathway
RA 3,468,138 T→G G572G (GGA→GGC chiA ← periplasmic endochitinase
RA 3,473,612 A→C L157* (TTA→TGA)  rpsG ← 30S ribosomal subunit protein S7
RA 3,515,115 A→C L267V (TTG→GTG)  dam ← DNA adenine methyltransferase
RA 3,520,438 A→C L89R (CTC→CGC)  hofQ ← DNA catabolic putative fimbrial transporter
RA 3,539,427 T→G S681S (TCT→TCG yhgF → putative transcriptional accessory protein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,577,196 T→G M133L (ATG→CTG)  gntK ← gluconate kinase 2
RA 3,602,989 T→G I419L (ATT→CTT)  ftsY ← Signal Recognition Particle (SRP) receptor
RA 3,610,025 A→C D24A (GAC→GCC)  dcrB → putative lipoprotein
RA 3,623,709 T→G I104S (ATT→AGT)  yhhH → putative NTF2 fold immunity protein for polymorphic toxin RhsB
RA 3,644,167 T→G E339A (GAA→GCA)  prlC ← oligopeptidase A
RA 3,679,181 T→G R3R (AGG→CGG)  yhjH ← cyclic‑di‑GMP phosphodiesterase, FlhDC‑regulated
RA 3,689,996 A→C L89* (TTA→TGA)  bcsZ ← endo‑1,4‑D‑glucanase
RA 3,697,854 A→C Q466P (CAA→CCA)  bcsE → cellulose production protein
RA 3,698,520 A→C T103P (ACC→CCC)  bcsG → DUF3260 family cellulose production inner membrane protein
RA 3,716,242 A→C V51V (GTT→GTG bisC ← biotin sulfoxide reductase
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine tRNA synthetase, alpha subunit
RA 3,749,326 A→C D32A (GAT→GCT)  sgbU → putative L‑xylulose 5‑phosphate 3‑epimerase
RA 3,752,058 A→C I23L (ATC→CTC)  yiaU → putative DNA‑binding transcriptional regulator
JC JC 3,765,971 IS2 (+) +5 bp coding (3789‑3793/4134 nt) rhsA → Rhs protein with putative toxin 55 domain; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
RA 3,766,247 A→C K1355N (AAA→AAC rhsA → Rhs protein with putative toxin 55 domain; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
RA 3,790,919 A→C L143V (TTA→GTA)  tdh ← L‑threonine 3‑dehydrogenase, NAD(P)‑binding
RA 3,812,232 A→C F110V (TTT→GTT)  yicR ← UPF0758 family protein
JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
RA 3,823,774 A→C N459H (AAC→CAC)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,871,503 A→C D76E (GAT→GAG dgoT ← D‑galactonate transporter
RA 3,966,875 T→G S153S (TCT→TCG rho → transcription termination factor
RA 3,967,461 A→C I349L (ATC→CTC)  rho → transcription termination factor
RA 3,972,452 A→C Q398P (CAG→CCG)  wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
RA 3,988,989 T→G A26A (GCA→GCC hemX ← putative uroporphyrinogen III methyltransferase
RA 4,008,222 A→C I156L (ATC→CTC)  rhtC → threonine efflux pump
RA 4,011,824 T→G L250R (CTG→CGG)  yigM → putative inner membrane EamA‑like transporter
RA 4,012,707 A→C F37C (TTT→TGT)  metR ← methionine biosynthesis regulon transcriptional regulator
RA 4,046,716 A→C intergenic (‑114/‑250) yihG ← / → polA inner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease
RA 4,061,235 A→C Y24S (TAT→TCT)  yihM → putative sugar phosphate isomerase
RA 4,143,778 A→C E52A (GAA→GCA)  frwB → putative enzyme IIB component of PTS
RA 4,186,603 A→C E418D (GAA→GAC rpoC → RNA polymerase, beta prime subunit
RA 4,190,100 T→G I105M (ATT→ATG yjaZ → stationary phase growth adaptation protein
RA 4,215,122 T→G G281G (GGT→GGG metA → homoserine O‑transsuccinylase
RA 4,216,808 T→G L444R (CTG→CGG)  aceB → malate synthase A
RA 4,245,692 A→C F243C (TTT→TGT)  malE ← maltose transporter subunit
RA 4,285,713 A→C N553K (AAT→AAG acs ← acetyl‑CoA synthetase
RA 4,291,339 T→G I288M (ATT→ATG nrfD → formate‑dependent nitrite reductase, membrane subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,313,090 T→G intergenic (‑99/‑260) alsR ← / → rpiB d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR/ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
RA 4,331,998 T→G D499E (GAT→GAG proP → proline/glycine betaine transporter
RA 4,385,478 A→C E29A (GAG→GCG)  yjeN → uncharacterized protein
RA 4,403,148 A→C N371H (AAC→CAC)  hflK → modulator for HflB protease specific for phage lambda cII repressor
RA 4,452,800 T→G I77S (ATC→AGC)  ytfT → putative sugar ABC transporter permease
RA 4,464,914 T→G I423L (ATC→CTC)  treB ← trehalose‑specific PTS enzyme: IIB and IIC component
RA 4,475,797 A→C I121L (ATT→CTT)  yjgL → SopA‑central‑domain‑like hexapeptide repeat protein
RA 4,476,115 A→C N227H (AAT→CAT)  yjgL → SopA‑central‑domain‑like hexapeptide repeat protein
RA 4,478,193 T→G E40A (GAA→GCA)  argI ← ornithine carbamoyltransferase 1
RA 4,499,550 A→C intergenic (+50/+43) insD1 → / ← yjgX IS2 transposase TnpB/pseudogene fragment
RA 4,522,275 T→G H607P (CAT→CCT)  yjhG ← putative dehydratase
RA 4,532,668 T→G G172G (GGA→GGC yjhP ← putative methyltransferase
RA 4,548,617 A→C L111L (CTA→CTC fimG → minor component of type 1 fimbriae
RA 4,595,235 A→C F206C (TTT→TGT)  lgoR ← putative transcriptional activator for L‑galactonate catabolism
RA 4,613,561 C→T R27R (CGC→CGT yjjV → putative DNase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423700–3424234 3424610–3424238 5–911 92 [88] [90] 92 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)156 (1.080) 91/182 0.2 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
* ? NC_000913 1207790 =53 (0.340)94 (0.710) 65/166 1.2 73.0% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 25 (0.190)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)191 (1.270) 101/190 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein