Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,869,491 A→C L10R (CTG→CGG)  pcm ← L‑isoaspartate protein carboxylmethyltransferase type II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,869,4910AC98.7% 557.6 / ‑3.2 158L10R (CTG→CGG) pcmL‑isoaspartate protein carboxylmethyltransferase type II
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base C (78/77);  total (79/79)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 158 total aligned reads displayed.

TTGTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCAGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGTTAAATC  >  NC_000913/2869394‑2869587
                                                                                                 |                                                                                                
 tGTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGaa                                                                                               >  2:954131/1‑100 (MQ=255)
  gTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAGGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAg                                                                                              <  1:1615589/100‑1 (MQ=255)
  gTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAg                                                                                              <  1:1850092/100‑1 (MQ=255)
  gTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAg                                                                                              <  1:269308/100‑1 (MQ=255)
  gTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAg                                                                                              <  1:3924106/100‑1 (MQ=255)
  gTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAg                                                                                              >  1:1494093/1‑100 (MQ=255)
  gTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGAGCATTCAGCACCTGCTCACCCTGAATACCTTGCGCACGTAATTGATCCCGAAg                                                                                              <  1:3784318/100‑1 (MQ=255)
   ttCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGt                                                                                             >  1:3009540/1‑100 (MQ=255)
     cAAATGCTTCATCAACGAATTTTCCACGCGGCACGGCGGCAAGTGTATTCAGCACATGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGc                                                                                           >  2:396890/1‑100 (MQ=255)
     cAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATGCCTTGCGCACGTAATTGATCCCGAAGTGc                                                                                           >  2:190799/1‑100 (MQ=255)
      aaaCGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCt                                                                                          <  1:3021159/100‑1 (MQ=255)
       aaCGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCtt                                                                                         >  1:1156364/1‑100 (MQ=255)
        aCGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTg                                                                                        <  1:1238664/100‑1 (MQ=255)
        aCGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTg                                                                                        <  1:684558/100‑1 (MQ=255)
         cGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGt                                                                                       >  2:3825968/1‑100 (MQ=255)
          gCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTa                                                                                      <  2:2545845/100‑1 (MQ=255)
           cTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTAc                                                                                     <  1:3485599/100‑1 (MQ=255)
           cTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTAc                                                                                     <  2:27671/100‑1 (MQ=255)
              catcaACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTAcgcg                                                                                  >  1:2081426/1‑100 (MQ=255)
               atcaACGAATTTTTCACGCGGCACGGCGGCATGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGt                                                                                 >  2:1236019/1‑100 (MQ=255)
                tcaACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTc                                                                                <  1:1018084/100‑1 (MQ=255)
                tcaACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTc                                                                                <  2:248694/100‑1 (MQ=255)
                 caACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCt                                                                               <  1:438925/100‑1 (MQ=255)
                  aaCGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTg                                                                              <  2:2044156/100‑1 (MQ=255)
                  aaCGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTg                                                                              >  2:8517/1‑100 (MQ=255)
                   aCGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGc                                                                             <  2:1946125/100‑1 (MQ=255)
                    cGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCt                                                                            >  1:182674/1‑100 (MQ=255)
                     gAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCtt                                                                           >  1:1397726/1‑100 (MQ=255)
                       aTTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTAc                                                                         >  2:162550/1‑100 (MQ=255)
                       aTTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTAc                                                                         >  1:1810491/1‑100 (MQ=255)
                        tttttCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTAcc                                                                        <  1:2436994/100‑1 (MQ=255)
                        tttttCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTAcc                                                                        <  1:2665264/100‑1 (MQ=255)
                        tttttCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTAcc                                                                        <  1:1196774/100‑1 (MQ=255)
                          tttCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTCCGCGTCTGCTTCCCAt                                                                      >  2:1168265/1‑100 (MQ=255)
                             cACGCGGCACGGCGGCAAGTGCATTCTGCACCTGCTCATGCTGAAGACCTTGCGCACGTAATTGATCCCGGAGTGGTTGTACGCGTCTGCTTACCATTGc                                                                   <  1:3810213/100‑1 (MQ=255)
                              aCGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCg                                                                  >  2:854784/1‑100 (MQ=255)
                               cgcgGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGt                                                                 >  1:3990613/1‑100 (MQ=255)
                                 cgGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGc                                                               >  1:3320856/1‑100 (MQ=255)
                                 cgGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGc                                                               <  2:3082836/100‑1 (MQ=255)
                                      cggcggCAAGTGCATTCAGCACCTGCACATCCTGAATACCTTGCGCGCGTAATTGATCCCGAATTGCTTGTACGCATCTGCTTATCATTGCGTGCCAACt                                                          <  2:394218/100‑1 (MQ=255)
                                          ggcAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCa                                                      <  2:3580079/100‑1 (MQ=255)
                                          ggcAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCa                                                      >  1:2884300/1‑100 (MQ=255)
                                           gcAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCAc                                                     <  2:3047769/100‑1 (MQ=255)
                                              aGTACATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCt                                                  >  2:1835335/1‑100 (MQ=255)
                                               gTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGACGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTGCCACGCTg                                                 >  2:3862496/1‑100 (MQ=255)
                                                 gCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGtt                                               >  1:105906/1‑100 (MQ=255)
                                                 gCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGtt                                               >  1:1594140/1‑100 (MQ=255)
                                                 gCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGtt                                               >  1:356535/1‑100 (MQ=255)
                                                  cATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGttt                                              <  1:1688606/100‑1 (MQ=255)
                                                    ttCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTaa                                            <  2:1856156/100‑1 (MQ=255)
                                                    ttCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTaa                                            >  1:909750/1‑100 (MQ=255)
                                                      cAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAAcc                                          >  1:2120165/1‑100 (MQ=255)
                                                      cAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAAcc                                          <  1:3373578/100‑1 (MQ=255)
                                                        gCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAg                                        >  2:1324300/1‑100 (MQ=255)
                                                        gCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAg                                        <  2:2502845/100‑1 (MQ=255)
                                                        gCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAg                                        >  1:1726475/1‑100 (MQ=255)
                                                         cACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGt                                       >  2:806560/1‑100 (MQ=255)
                                                         cACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGt                                       >  1:2248997/1‑100 (MQ=255)
                                                         cACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGt                                       <  2:3936253/100‑1 (MQ=255)
                                                         cACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACCCTGTTTAACCAGt                                       >  2:3182537/1‑100 (MQ=255)
                                                          aCCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTc                                      >  1:2878616/1‑100 (MQ=255)
                                                          aCCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCAGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTc                                      <  2:277590/100‑1 (MQ=255)
                                                             tGCTCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGa                                   >  1:2973340/1‑100 (MQ=255)
                                                                tCATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAAc                                <  2:3667477/100‑1 (MQ=255)
                                                                 cATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAAcc                               >  2:2322116/1‑100 (MQ=255)
                                                                 cATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAAcc                               <  2:3282349/100‑1 (MQ=255)
                                                                 cATCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCCTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTACCCAGCCTGAAAcc                               >  2:2707429/1‑100 (MQ=255)
                                                                 cATCCTGAAGACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCTACTCTGTTTAACCAGTCTGAAAca                               <  2:1437161/100‑2 (MQ=255)
                                                                  aTCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACca                              >  2:2553410/1‑100 (MQ=255)
                                                                  aTCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACca                              >  1:3431702/1‑100 (MQ=255)
                                                                  aTCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTACGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACca                              >  2:2218785/1‑100 (MQ=255)
                                                                  aTCCTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCAGTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACca                              <  1:3109400/100‑1 (MQ=255)
                                                                    ccTGAATACCTTGCGCACGTAATTGATCCCGACGTGCTGGTACGCGGCTGCTTACCATTGCGTGCCAACACCCACGCTGTGTAACCAGTCTGAAACcaca                            >  1:3621858/1‑100 (MQ=255)
                                                                    ccTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACcaca                            >  1:2291677/1‑100 (MQ=255)
                                                                    ccTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACcaca                            >  2:680965/1‑100 (MQ=255)
                                                                     cTGAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACAt                           <  2:3325516/100‑1 (MQ=255)
                                                                       gAATACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCt                         <  2:3224537/100‑1 (MQ=255)
                                                                         aTACCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTg                       <  1:1777907/100‑1 (MQ=255)
                                                                           aCCTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTgcg                     <  1:1566128/100‑1 (MQ=255)
                                                                            ccTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACTAGTCTGAAACCACATCTTgcgc                    >  1:3060894/1‑100 (MQ=255)
                                                                            ccTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTgcgc                    >  2:1079312/1‑100 (MQ=255)
                                                                             cTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTgcgcg                   <  2:1591881/100‑1 (MQ=255)
                                                                             cTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTgcgcg                   <  1:2593040/100‑1 (MQ=255)
                                                                             cTTGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTgcgcg                   <  1:635322/100‑1 (MQ=255)
                                                                               tGCGCACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCt                 >  1:3328687/1‑100 (MQ=255)
                                                                                gcgcACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTa                <  1:329435/100‑1 (MQ=255)
                                                                                 cgcACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTAt               <  1:330362/100‑1 (MQ=255)
                                                                                 cgcACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTAt               >  1:2326599/1‑100 (MQ=255)
                                                                                 cgcACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTAt               <  2:819500/100‑1 (MQ=255)
                                                                                 cgcACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTACCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTAt               >  1:1147161/1‑100 (MQ=255)
                                                                                  gcACGTAATTGATCCTGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATg              >  2:3049445/1‑100 (MQ=255)
                                                                                   cACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATgc             >  1:166705/1‑100 (MQ=255)
                                                                                   cACGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATgc             <  2:1785968/100‑1 (MQ=255)
                                                                                     cGTAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATgcgc           >  1:3230375/1‑100 (MQ=255)
                                                                                       tAATTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAg         >  1:2292806/1‑100 (MQ=255)
                                                                                        aaTTGCTCCCGAAGCGCTTGTCCGCGTCTGCTTACCCTTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGt        <  1:823907/100‑1 (MQ=255)
                                                                                        aaTTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGt        <  1:2358378/100‑1 (MQ=255)
                                                                                        aaTTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGt        <  1:2548829/100‑1 (MQ=255)
                                                                                         aTTGATCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGtt       >  2:3478545/1‑100 (MQ=255)
                                                                                             aTCCCGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGTTAAAt   >  2:6792/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TTGTTCAAACGCTTCATCAACGAATTTTTCACGCGGCACGGCGGCAAGTGCATTCAGCACCTGCTCATCCTGAATACCTTGCGCACGTAATTGATCCAGAAGTGCTTGTACGCGTCTGCTTACCATTGCGTGCCAACTCCCACGCTGTTTAACCAGTCTGAAACCACATCTTGCGCGCTATGCGCAGTTAAATC  >  NC_000913/2869394‑2869587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A