Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,988,621 T→G L40L (CTT→CTG)  yqeI → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,988,6210TG93.0% 438.6 / 18.8 143L40L (CTT→CTG) yqeIputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (5/5);  new base G (53/79);  total (59/84)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 143 total aligned reads displayed.

ATATCGAGTTCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTTCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTAACACGTTATA  >  NC_000913/2988524‑2988716
                                                                                                 |                                                                                                
atatCGAGTTCTGGCCTGAGCACCGAAACTTAATATCGGTACAGAACGCCGATCTTAACGTCGTGCTGACAACGCCAGCCAGTCGATGTTTATCACTGCt                                                                                                <  2:1219651/100‑1 (MQ=255)
 tatCGAGTTCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTa                                                                                               <  2:230710/100‑1 (MQ=255)
 tatCGAGTTCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTa                                                                                               >  1:163708/1‑100 (MQ=255)
  atCGAGTTCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTAc                                                                                              >  2:3385224/1‑100 (MQ=255)
  atCGAGTTCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTAc                                                                                              >  1:3789757/1‑100 (MQ=255)
   tCGAGTTCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACt                                                                                             <  1:591614/100‑1 (MQ=255)
   tCGAGTTCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACt                                                                                             <  1:486597/100‑1 (MQ=255)
    cGAGTTCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTTCTACtt                                                                                            <  2:2546438/100‑1 (MQ=255)
    cGAGTTCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACtt                                                                                            >  2:3795837/1‑100 (MQ=255)
     gAGTTCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTg                                                                                           <  2:3767150/100‑1 (MQ=255)
     gAGTTCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGAGCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTg                                                                                           <  1:3971765/100‑1 (MQ=255)
        ttCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTTCTACTTGAAg                                                                                        <  2:3280936/100‑1 (MQ=255)
        ttCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAg                                                                                        <  2:3433634/100‑1 (MQ=255)
            ggCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCttt                                                                                    >  2:2265216/1‑100 (MQ=255)
            ggCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCttt                                                                                    >  2:2556512/1‑100 (MQ=255)
            ggCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCttt                                                                                    >  1:467358/1‑100 (MQ=255)
            ggCCTAAGCACCGAAAATTAATAACGGTACATAACGCCGATCTTAACGTCTGTCGGACAACGCCACCCAGTCGCTGTGTATCACTCCTACTTGAAGCttt                                                                                    >  2:514928/1‑100 (MQ=255)
               cTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTcc                                                                                 <  1:2533741/100‑1 (MQ=255)
                tGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCt                                                                                <  1:739967/100‑1 (MQ=255)
                tGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCt                                                                                >  1:3550755/1‑100 (MQ=255)
                 gAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTg                                                                               <  2:824100/100‑1 (MQ=255)
                    cACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGAtg                                                                            <  1:145595/100‑1 (MQ=255)
                    cACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGAtg                                                                            <  1:1901644/100‑1 (MQ=255)
                      ccGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGAtgtg                                                                          >  2:2980696/1‑100 (MQ=255)
                      ccGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGAtgtg                                                                          <  1:202321/100‑1 (MQ=255)
                      ccGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGAtgtg                                                                          >  1:155317/1‑100 (MQ=255)
                          aaaTTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTg                                                                      <  1:3454685/100‑1 (MQ=255)
                          aaaTTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTg                                                                      <  1:3023155/100‑1 (MQ=255)
                           aaTTAATATCGGTACATAGCGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTCGTTGc                                                                     <  2:3517717/100‑1 (MQ=255)
                                atatCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCacaac                                                                >  2:1587034/1‑100 (MQ=255)
                                 tatCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCacaaca                                                               <  2:783606/100‑1 (MQ=255)
                                  atCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCAcaacaa                                                              >  2:865134/1‑100 (MQ=255)
                                  atCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCAcaacaa                                                              >  1:3758501/1‑100 (MQ=255)
                                  atCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTGTATCACTGCTACTTGAAGCGTTTCCTGATGTGGTTGCAcaacaa                                                              <  2:700459/100‑1 (MQ=255)
                                   tCGGTACATAACGCCGATCTTAACGTCGTTCTGACACCGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAg                                                             >  1:2873983/1‑100 (MQ=255)
                                   tCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAg                                                             <  1:1555790/100‑1 (MQ=255)
                                    cGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGa                                                            >  1:215274/1‑100 (MQ=255)
                                     ggTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑t                                                          <  2:3228058/100‑1 (MQ=255)
                                     ggTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑t                                                          >  1:174080/1‑100 (MQ=255)
                                        aCATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑tttt                                                       <  2:3709592/100‑1 (MQ=255)
                                         cATAACGCCGATCTTCACGTCCTTCTGACAACACCAGCCAGTCGATGTTTATCACTGCTACTTCAGGCTTTTCCTGATGTGGTTGCACAACAAGA‑ttttt                                                      >  2:2822866/1‑100 (MQ=255)
                                         cATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGGCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑ttttt                                                      <  2:2139274/100‑1 (MQ=255)
                                           tAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTc                                                    >  1:3221868/1‑100 (MQ=255)
                                           tAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTc                                                    >  2:3646614/1‑100 (MQ=255)
                                              cGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTTCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCAcc                                                 <  1:1858812/100‑1 (MQ=255)
                                              cGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCAcc                                                 >  1:2360179/1‑100 (MQ=255)
                                              cGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCAcc                                                 <  2:1323708/100‑1 (MQ=255)
                                              cGCCGATCTTAACGTCGTTCTGACAACGCCAGACAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCAcc                                                 >  2:2397306/1‑100 (MQ=255)
                                               gCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCa                                                <  1:3945203/100‑1 (MQ=255)
                                               gCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCa                                                <  2:2889580/100‑1 (MQ=255)
                                                ccGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCag                                               <  2:649529/100‑1 (MQ=255)
                                                ccGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCag                                               <  1:1911815/100‑1 (MQ=255)
                                                ccGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCag                                               <  1:947368/100‑1 (MQ=255)
                                                  gATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTTCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCagag                                             >  2:27216/1‑100 (MQ=255)
                                                  gATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCagag                                             >  1:2954702/1‑100 (MQ=255)
                                                  gATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCagag                                             <  1:2824336/100‑1 (MQ=255)
                                                     cTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTTCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCt                                          >  2:1846194/1‑100 (MQ=255)
                                                      ttAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTg                                         >  1:3966074/1‑100 (MQ=255)
                                                        aaCGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTggg                                       <  1:970648/100‑1 (MQ=255)
                                                        aaCGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTggg                                       <  1:2767814/100‑1 (MQ=255)
                                                        aaCGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTggg                                       <  2:1054169/100‑1 (MQ=255)
                                                         aCGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGga                                      <  2:331373/100‑1 (MQ=255)
                                                          cgtcgtTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGgaa                                     <  1:264765/100‑1 (MQ=255)
                                                            tcgtTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCCCCAGATTCTGGgaaga                                   <  1:3571197/100‑1 (MQ=255)
                                                            tcgtTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGgaaga                                   <  1:3205830/100‑1 (MQ=255)
                                                              gtTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGgaagaag                                 >  2:1420910/1‑100 (MQ=255)
                                                              gtTCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGgaagaag                                 >  1:982409/1‑100 (MQ=255)
                                                               ttCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGgaagaaga                                <  2:1066051/100‑1 (MQ=255)
                                                               ttCTGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGgaagaaga                                <  1:3028817/100‑1 (MQ=255)
                                                                  tGACCACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAgg                             >  2:3510328/1‑100 (MQ=255)
                                                                  tGACAACGCCAGCCAGTCGATGTTTATCACTTCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAgg                             <  1:1799378/100‑1 (MQ=255)
                                                                  tGACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAgg                             <  2:2222307/100‑1 (MQ=255)
                                                                   gACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGt                            >  1:1651669/1‑100 (MQ=255)
                                                                   gACAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGt                            >  2:3922998/1‑100 (MQ=255)
                                                                     cAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTAt                          <  2:3531909/100‑1 (MQ=255)
                                                                     cAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTAt                          >  1:3052198/1‑100 (MQ=255)
                                                                     cAACGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTAt                          >  2:3545592/1‑100 (MQ=255)
                                                                      aaCGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATg                         >  2:2969277/1‑100 (MQ=255)
                                                                        cGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCg                       <  1:503272/100‑1 (MQ=255)
                                                                        cGCCAGCCAGTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCg                       <  1:347876/100‑1 (MQ=255)
                                                                          ccagccagTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCgtg                     <  1:2363886/100‑1 (MQ=255)
                                                                          ccagccagTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCgtg                     <  1:623251/100‑1 (MQ=255)
                                                                            agccagTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGtgtg                   <  2:194375/100‑1 (MQ=255)
                                                                             gccagTCGATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGc                  <  1:753319/100‑1 (MQ=255)
                                                                              ccagTCGATGTTTATCACTGCTACTTAAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGTAGAAGAAGGTATGCGTGTGcc                 >  2:3934945/1‑100 (MQ=255)
                                                                                    gATGTTTATCACTTCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTaa           >  2:1544647/1‑100 (MQ=255)
                                                                                    gATGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTaa           >  2:1267152/1‑100 (MQ=255)
                                                                                     aTGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTATTAac          <  2:1624078/100‑1 (MQ=255)
                                                                                     aTGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTAac          <  1:1028665/100‑1 (MQ=255)
                                                                                      tGTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTAaca         <  1:2910940/100‑1 (MQ=255)
                                                                                       gTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTAacac        >  2:2756194/1‑100 (MQ=255)
                                                                                       gTTTATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTAacac        <  1:1244001/100‑1 (MQ=255)
                                                                                       gTTTATCACTGCTACTTGAAGCTTTTCCTGACGTGGTTGCACAAGAAGA‑TTTTTTGACCAGTGTCGGGGAAGAAGAAGGTATGCGTGTGCCTACTAacac        >  1:3925648/1‑100 (MQ=255)
                                                                                        tttATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTAACACg       >  1:2664807/1‑100 (MQ=255)
                                                                                        tttATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTAACACg       >  1:2556996/1‑100 (MQ=255)
                                                                                         ttATCACTGCTACTTGAAGCTTTTCCGGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTAACACGt      <  1:1982974/100‑1 (MQ=255)
                                                                                         tgaTCACTGCTGCTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTAACACGt      >  2:3701876/3‑100 (MQ=255)
                                                                                          tATCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGATTTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTAACACGt      <  1:384109/100‑1 (MQ=255)
                                                                                           aTCACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTAACACGTta    >  2:2091411/1‑100 (MQ=255)
                                                                                             cACTGCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGACGAAGAAGGTATGCGTGTGCCTACTAACACGTtata  >  2:3938043/1‑100 (MQ=255)
                                                                                                 |                                                                                                
ATATCGAGTTCTGGCCTGAGCACCGAAAATTAATATCGGTACATAACGCCGATCTTAACGTCGTTCTGACAACGCCAGCCAGTCGATGTTTATCACTTCTACTTGAAGCTTTTCCTGATGTGGTTGCACAACAAGA‑TTTTTTCACCAGAGTCTGGGAAGAAGAAGGTATGCGTGTGCCTACTAACACGTTATA  >  NC_000913/2988524‑2988716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A