Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,197,496 T→G T716P (ACT→CCT)  glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,197,4960TG97.6% 572.4 / ‑2.8 169T716P (ACT→CCT) glnEfused deadenylyltransferase/adenylyltransferase for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base G (77/88);  total (79/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 172 total aligned reads displayed.

AAAATGCCGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGTCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGC  >  NC_000913/3197399‑3197592
                                                                                                 |                                                                                                
aaaaTGCCGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCgtcaggca                                                                                                >  1:3362573/1‑100 (MQ=255)
aaaaTGCCGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCgtcaggca                                                                                                >  1:1411982/1‑100 (MQ=255)
 aaaTGCCGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCAt                                                                                               <  2:3077674/100‑1 (MQ=255)
   aTGCCGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCg                                                                                             <  1:2393094/100‑1 (MQ=255)
   aTGCCGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCg                                                                                             >  1:1577447/1‑100 (MQ=255)
    tGCCGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGc                                                                                            >  2:688619/1‑100 (MQ=255)
        ngAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATcc                                                                                        >  1:3252893/2‑100 (MQ=255)
            gagGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTg                                                                                    <  1:2514836/100‑1 (MQ=255)
            gagGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTg                                                                                    <  1:727823/100‑1 (MQ=255)
            gagGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTg                                                                                    <  2:1979328/100‑1 (MQ=255)
              ggTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTggg                                                                                  >  1:4054160/1‑100 (MQ=255)
              ggTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTggg                                                                                  >  1:1382922/1‑100 (MQ=255)
               gTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGc                                                                                 <  2:2432202/100‑1 (MQ=255)
               gTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGc                                                                                 <  1:2427405/100‑1 (MQ=255)
                    cgTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCa                                                                            <  1:1167552/100‑1 (MQ=255)
                     ntACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCAt                                                                           >  1:1851280/2‑100 (MQ=255)
                     gTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATcct                                                                           >  2:3542158/1‑98 (MQ=255)
                      tACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGTCATCGCATCCATTGGGCAATCATg                                                                          >  2:2600297/1‑100 (MQ=255)
                      tACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATg                                                                          >  2:3472150/1‑100 (MQ=255)
                        cTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATgga                                                                        <  1:1868965/100‑1 (MQ=255)
                        cTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATgga                                                                        >  1:1767266/1‑100 (MQ=255)
                        cTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCAAgga                                                                        <  1:3139590/100‑1 (MQ=255)
                         tGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATggag                                                                       <  2:784920/100‑1 (MQ=255)
                         tGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATggag                                                                       <  2:813903/100‑1 (MQ=255)
                          gAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATggagg                                                                      >  2:1695478/1‑100 (MQ=255)
                          gAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATggagg                                                                      >  2:2740162/1‑100 (MQ=255)
                          gAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTCCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATggagg                                                                      >  2:760462/1‑100 (MQ=255)
                            tcAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGaa                                                                    <  1:4086538/99‑1 (MQ=255)
                            aCAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGaa                                                                    <  1:22439/100‑1 (MQ=255)
                            aCAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGaa                                                                    <  2:3370186/100‑1 (MQ=255)
                             cAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAg                                                                   <  1:964472/100‑1 (MQ=255)
                             cAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAg                                                                   >  1:195711/1‑100 (MQ=255)
                              aGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGa                                                                  <  1:3959653/100‑1 (MQ=255)
                               gATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGAt                                                                 >  1:641716/1‑100 (MQ=255)
                               gATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGAt                                                                 <  2:550316/100‑1 (MQ=255)
                               gATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGAt                                                                 >  1:2899113/1‑100 (MQ=255)
                                aTGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATa                                                                <  2:1692259/100‑1 (MQ=255)
                                 tGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATaa                                                               <  1:2891199/100‑1 (MQ=255)
                                  gCATAATGCGTTGCGCCAGACGTAGATATAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAg                                                              <  1:3713046/100‑1 (MQ=255)
                                  gCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAg                                                              <  2:1711343/100‑1 (MQ=255)
                                    aTAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGt                                                            <  2:2958988/100‑1 (MQ=255)
                                     tAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTc                                                           <  1:782880/100‑1 (MQ=255)
                                     tAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTc                                                           >  2:1626167/1‑100 (MQ=255)
                                       aTGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCaa                                                         <  1:2663150/100‑1 (MQ=255)
                                          cGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGAt                                                      >  1:2567065/1‑100 (MQ=255)
                                            ttGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCTGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCg                                                    <  2:3615716/100‑1 (MQ=255)
                                            ttGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCg                                                    <  2:3140973/100‑1 (MQ=255)
                                             tGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCgg                                                   <  1:2099044/100‑1 (MQ=255)
                                             tGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCgg                                                   <  2:1755561/100‑1 (MQ=255)
                                                gcCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCCTTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAAc                                                >  2:2839125/1‑100 (MQ=255)
                                                gcCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAAc                                                <  2:1379115/100‑1 (MQ=255)
                                                gcCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAAc                                                >  2:3840431/1‑100 (MQ=255)
                                                gcCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAAc                                                <  2:1550677/100‑1 (MQ=255)
                                                 ccAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACt                                               >  1:3296739/1‑100 (MQ=255)
                                                  cAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTg                                              <  1:3380758/100‑1 (MQ=255)
                                                    gACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTa                                            >  1:1590895/1‑100 (MQ=255)
                                                     aCGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAg                                           <  2:3179683/100‑1 (MQ=255)
                                                     aCGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAg                                           <  1:145566/100‑1 (MQ=255)
                                                       gCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGTCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGcc                                         <  2:2699976/100‑1 (MQ=255)
                                                        cAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCt                                        <  1:2034034/100‑1 (MQ=255)
                                                        cAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCt                                        >  1:35729/1‑100 (MQ=255)
                                                        atcttAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCt                                        <  2:1412890/96‑1 (MQ=255)
                                                         aGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTa                                       <  2:1434110/100‑1 (MQ=255)
                                                         aGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTa                                       <  2:1358286/100‑1 (MQ=255)
                                                         aGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTa                                       >  1:2562652/1‑100 (MQ=255)
                                                          gATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGTCAATCATGTAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTaa                                      >  2:1884237/1‑100 (MQ=255)
                                                          gATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTaa                                      >  1:4006390/1‑100 (MQ=255)
                                                          gATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTaa                                      >  1:1498797/1‑100 (MQ=255)
                                                           ctAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAAc                                     <  2:919053/99‑1 (MQ=255)
                                                           aTAAAACTGCCGCCGGTCGATTTCCCGCTCACCGTCAGGCATCGCATACATTGGACAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAAc                                     >  2:3742405/1‑100 (MQ=255)
                                                           aTAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAAc                                     <  1:1722413/100‑1 (MQ=255)
                                                            tAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGTCATCGAATCCATTGGGCAATCATGTAGGAAGATAAGGCCAAGATCGGACCTGTCGCATaaat                                    >  2:1810311/1‑98 (MQ=255)
                                                             aaaaCTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTc                                   >  2:533613/1‑100 (MQ=255)
                                                              aaaCTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTcc                                  <  1:518622/100‑1 (MQ=255)
                                                               aaCTGCCGCCCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTccc                                 >  1:2409229/1‑100 (MQ=255)
                                                                   gccgccCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAgcc                             <  1:176970/100‑1 (MQ=255)
                                                                    ccgccCGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAgccg                            <  1:437237/100‑1 (MQ=255)
                                                                        ccGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCa                        <  1:1082903/100‑1 (MQ=255)
                                                                        ccGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCa                        <  1:1870345/100‑1 (MQ=255)
                                                                         cGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAg                       >  2:522200/1‑100 (MQ=255)
                                                                         cGTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAg                       <  2:1361497/100‑1 (MQ=255)
                                                                          gTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGc                      <  2:2762645/100‑1 (MQ=255)
                                                                          gTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGc                      >  1:1832721/1‑100 (MQ=255)
                                                                          gTCGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGc                      >  1:2805066/1‑100 (MQ=255)
                                                                            cGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCtt                    >  1:1096852/1‑100 (MQ=255)
                                                                            cGATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCtt                    >  2:2325921/1‑100 (MQ=255)
                                                                             gATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTg                   <  1:748469/100‑1 (MQ=255)
                                                                             gATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTg                   >  1:592823/1‑100 (MQ=255)
                                                                             gATTTCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTg                   <  1:254340/100‑1 (MQ=255)
                                                                               tttCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGcc                 <  2:3508551/100‑1 (MQ=255)
                                                                               tttCCCGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGcc                 >  2:98407/1‑100 (MQ=255)
                                                                                  cccGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTa              >  2:2079663/1‑100 (MQ=255)
                                                                                  cccGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTa              >  1:2815114/1‑100 (MQ=255)
                                                                                   ccGCTCACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAGCTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAg             >  1:3844260/1‑100 (MQ=255)
                                                                                        cACCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGac        >  1:673709/1‑100 (MQ=255)
                                                                                         aCCGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCGCAGCTTGCCGTAGCCGacc       <  1:3484245/100‑1 (MQ=255)
                                                                                          ccGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGacca      >  1:3945106/1‑100 (MQ=255)
                                                                                           cGTCAGGCCTCGTATCCAGTGGAAAATAAAGGATGAAGCTAAGGTGAAGAACGGAACTGTAGCCTAACTCCCAGCGGCCCAGATAGCCGCAGCGGaccac     >  2:1272873/1‑100 (MQ=255)
                                                                                           cGTCAGGCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGaccac     >  2:3253053/1‑100 (MQ=255)
                                                                                              caggcaTCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGGCGCCCAGCTTGCCGTAGCCGACCACCGc  >  1:3711820/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AAAATGCCGGAAGAGGTACGCGTACTGAACAGATGCATAATGCGTTGCGCCAGACGCAGATAAAACTGCCGCCCGTCGATTTCCCGCTCACCGTCAGTCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGC  >  NC_000913/3197399‑3197592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A