Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,198,644 A→C F333C (TTC→TGC)  glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,198,6440AC95.6% 507.0 / 7.7 160F333C (TTC→TGC) glnEfused deadenylyltransferase/adenylyltransferase for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base A (2/5);  new base C (79/72);  total (82/78)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 163 total aligned reads displayed.

TCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGAACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCC  >  NC_000913/3198545‑3198740
                                                                                                   |                                                                                                
  gTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCac                                                                                                <  2:714826/100‑1 (MQ=255)
  gTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCac                                                                                                <  1:3364329/100‑1 (MQ=255)
  gTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCac                                                                                                <  2:314777/100‑1 (MQ=255)
   ttttCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGcaca                                                                                               >  2:3097108/1‑100 (MQ=255)
     ttCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACAcc                                                                                             <  2:2094445/100‑1 (MQ=255)
        aGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGa                                                                                          >  1:1973696/1‑100 (MQ=255)
        aGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGa                                                                                          >  1:3104275/1‑100 (MQ=255)
        aGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGa                                                                                          >  1:846233/1‑100 (MQ=255)
        aGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGa                                                                                          >  2:3329818/1‑100 (MQ=255)
         gAAAGCAGATGCAGCTCGGCAATGGCGGTGAGCGTGGGCAGTAAAGAGCGCGAGTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGaa                                                                                         >  2:1715566/1‑100 (MQ=255)
         gAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGaa                                                                                         >  1:1785922/1‑100 (MQ=255)
         gAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGaa                                                                                         <  2:3059197/100‑1 (MQ=255)
           aaGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACg                                                                                       <  2:3002634/100‑1 (MQ=255)
           aaGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACg                                                                                       <  1:2587747/100‑1 (MQ=255)
           aaGCACATGCAGCTCGGCAATGGCGCTGAGGGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGATCg                                                                                       <  2:3011/100‑1 (MQ=255)
              cAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATa                                                                                    >  2:3735925/1‑100 (MQ=255)
              cAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATa                                                                                    <  2:411706/100‑1 (MQ=255)
              cAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATa                                                                                    <  1:222124/100‑1 (MQ=255)
              cAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATa                                                                                    <  1:1002862/100‑1 (MQ=255)
              cAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAACGAGCTGGCACACCTGAACGATa                                                                                    <  1:3417235/100‑1 (MQ=255)
               aGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATaa                                                                                   <  1:952854/100‑1 (MQ=255)
                gATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATaaa                                                                                  <  1:1333049/100‑1 (MQ=255)
                gATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGT‑AAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAAt                                                                                 <  1:2432205/100‑1 (MQ=255)
                   gCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTc                                                                               >  2:3620651/1‑100 (MQ=255)
                    cAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCa                                                                              <  1:692899/100‑1 (MQ=255)
                      gCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAAt                                                                            >  2:1572380/1‑100 (MQ=255)
                      gCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAAt                                                                            <  1:2902127/100‑1 (MQ=255)
                       cTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAAtt                                                                           >  1:3789498/1‑100 (MQ=255)
                        tCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAAttt                                                                          <  1:1505169/100‑1 (MQ=255)
                          ggCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTcg                                                                        <  1:2518254/100‑1 (MQ=255)
                          ggCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTcg                                                                        <  1:485647/100‑1 (MQ=255)
                          ggCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTcg                                                                        >  1:971580/1‑100 (MQ=255)
                           gCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTcgc                                                                       <  2:1150679/100‑1 (MQ=255)
                           gCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTcgc                                                                       >  1:3315282/1‑100 (MQ=255)
                           gCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGAACACCTGAACGATAAATTCAATTTcgc                                                                       <  2:353487/100‑1 (MQ=255)
                            gaaTGGCGCTGAGCGTTGTCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGGCCGCCGGGAATGAGCTGGCACACCTGAACGATAAATTCAATTTcgcg                                                                      <  2:2544008/99‑1 (MQ=255)
                            cAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGGGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTcgcg                                                                      <  2:260503/100‑1 (MQ=255)
                            cAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTcgcn                                                                      <  1:3237173/100‑2 (MQ=255)
                            cAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTcgcg                                                                      <  1:841771/100‑1 (MQ=255)
                            cAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGAACACCTGAACGATAAATTCAATTTcgcg                                                                      <  2:2873694/100‑1 (MQ=255)
                              aTGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGaa                                                                    >  2:1249352/1‑100 (MQ=255)
                              aTGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGaa                                                                    >  1:2961213/1‑100 (MQ=255)
                              aTGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGaa                                                                    >  1:90318/1‑100 (MQ=255)
                                   gcTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATgccg                                                               >  2:2658040/1‑100 (MQ=255)
                                     tGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATgccgcc                                                             >  1:16709/1‑100 (MQ=255)
                                     tGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATgccgcc                                                             <  1:2600707/100‑1 (MQ=255)
                                       aGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTg                                                           >  2:1855737/1‑100 (MQ=255)
                                         cGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTgcg                                                         <  2:227820/100‑1 (MQ=255)
                                           ttGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGcc                                                       >  1:1544339/1‑100 (MQ=255)
                                           ttGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGAACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGcc                                                       <  1:1533356/100‑1 (MQ=255)
                                              gCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGACTGCCGCCTGCGCCGAg                                                    >  2:2225313/1‑100 (MQ=255)
                                                 gTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGttt                                                 <  2:147270/100‑1 (MQ=255)
                                                 gTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGttt                                                 <  1:2184362/100‑1 (MQ=255)
                                                   aaaGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGa                                               <  1:2796735/100‑1 (MQ=255)
                                                     agagCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGata                                             <  2:2433740/100‑1 (MQ=255)
                                                      gagCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGAACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGatat                                            <  1:1383062/100‑1 (MQ=255)
                                                         cgcgATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTCATATTGt                                         >  1:3755180/1‑100 (MQ=255)
                                                           cgATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCg                                       >  1:136702/1‑100 (MQ=255)
                                                           cgATTGCAGCGACGGTTCGCGGCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCg                                       <  2:869187/100‑1 (MQ=255)
                                                             aTTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGt                                     >  1:3007706/1‑100 (MQ=255)
                                                              ttGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTc                                    <  1:147595/100‑1 (MQ=255)
                                                               tGCAGCGACGGTTCGCGTCCGGCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCa                                   <  2:1200172/100‑1 (MQ=255)
                                                                gCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCaa                                  <  2:2414847/100‑1 (MQ=255)
                                                                gCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCaa                                  >  1:593640/1‑100 (MQ=255)
                                                                 cAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGACTGCGCCGAGTTTGATATTGTCGGTCaaa                                 >  1:3723214/1‑100 (MQ=255)
                                                                 cAGCGACGGTTCGCGTCCGCCGCGAATCAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCaaa                                 >  1:3947248/1‑100 (MQ=255)
                                                                   gCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAAcc                               <  2:3310777/100‑1 (MQ=255)
                                                                   gCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAAcc                               >  1:1914239/1‑100 (MQ=255)
                                                                   gCGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAAcc                               <  1:3947627/100‑1 (MQ=255)
                                                                    cGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCa                              <  2:1391865/100‑1 (MQ=255)
                                                                    cGACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCa                              <  1:1384648/100‑1 (MQ=255)
                                                                     gACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCAc                             <  1:61836/100‑1 (MQ=255)
                                                                     gACGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATACCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCAc                             >  2:454632/1‑100 (MQ=255)
                                                                      aCGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACg                            <  1:1496498/100‑1 (MQ=255)
                                                                      aCGGTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACg                            <  1:205084/100‑1 (MQ=255)
                                                                       tcattCGCGGCGGACGCGAATGAGCTGGAACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGt                           >  1:3100658/4‑100 (MQ=255)
                                                                         gTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCg                         <  2:843903/100‑1 (MQ=255)
                                                                         gTTCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCg                         >  1:1883314/1‑100 (MQ=255)
                                                                          ttCGCGTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGa                        >  1:3408550/1‑100 (MQ=255)
                                                                            cgcgTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACg                      >  1:755697/1‑100 (MQ=255)
                                                                            cgcgTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACg                      >  1:1310495/1‑100 (MQ=255)
                                                                              cgTCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCa                    <  1:280622/100‑1 (MQ=255)
                                                                                tCCGCCGCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACt                  <  1:807685/100‑1 (MQ=255)
                                                                                 ccgccgCGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACtt                 <  2:689990/100‑1 (MQ=255)
                                                                                  cgccgcGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTc                >  2:969165/1‑100 (MQ=255)
                                                                                  cgccgcGAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTc                >  1:345149/1‑100 (MQ=255)
                                                                                       cgAATGAGCTGGGCCACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACggg           <  2:3412547/100‑1 (MQ=255)
                                                                                       cgAATGAGCTGGCACACCTGACCGATAGATTCAATTTCGCGAATGCCGCCTGCGCCGTGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACggg           <  2:763117/100‑1 (MQ=255)
                                                                                       cgAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACggg           <  2:1756961/100‑1 (MQ=255)
                                                                                       cgAATGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACggg           >  1:106920/1‑100 (MQ=255)
                                                                                       cgAAGGAGCTGGCACACCTGAACGAGAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACggg           <  2:2809821/100‑1 (MQ=255)
                                                                                         aaTGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCa         <  2:2133478/100‑1 (MQ=255)
                                                                                          atcaGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGCGTTTGATCTTGTCGGTCAAACCACGCCGACGCACTGCACGGGCaa        >  2:128539/4‑100 (MQ=255)
                                                                                          aTGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGTGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCaa        <  2:1408764/100‑1 (MQ=255)
                                                                                          aTGAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCaa        >  2:1422131/1‑100 (MQ=255)
                                                                                            gAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAatc      <  1:1737802/100‑1 (MQ=255)
                                                                                            gAGCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAatc      <  1:1599346/100‑1 (MQ=255)
                                                                                              gCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAACCCACGTCGACGCACTTCACGGGCAatcat    >  2:1464658/1‑100 (MQ=255)
                                                                                              gCTGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAatcat    >  1:2178433/1‑100 (MQ=255)
                                                                                                tGGCACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATcc  <  2:1517958/100‑1 (MQ=255)
                                                                                                   |                                                                                                
TCGTTTTCAGAAAGCAGATGCAGCTCGGCAATGGCGCTGAGCGTTGGCAGTAAAGAGCGCGATTGCAGCGACGGTTCGCGTCCGCCGCGAATGAGCTGGAACACCTGAACGATAAATTCAATTTCGCGAATGCCGCCTGCGCCGAGTTTGATATTGTCGGTCAAACCACGTCGACGCACTTCACGGGCAATCATCC  >  NC_000913/3198545‑3198740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A