Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,334,314 A→C Y202S (TAT→TCT)  ispB → octaprenyl diphosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,334,3140AC93.9% 510.4 / 16.2 164Y202S (TAT→TCT) ispBoctaprenyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (5/5);  new base C (77/77);  total (82/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 166 total aligned reads displayed.

GCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAG  >  NC_000913/3334215‑3334409
                                                                                                   |                                                                                               
gCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTa                                                                                                 >  1:2121748/1‑100 (MQ=255)
    aaCCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGctctctn                                                                                             <  1:3262287/100‑2 (MQ=255)
     gccGCGCGTCTGTTTGAGGCTGCCGCGCAGGGGTCCGGGATTCTGGCTGGCTGTACGGCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCg                                                                                            <  2:1624439/99‑1 (MQ=255)
     aCCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCg                                                                                            <  2:3504719/100‑1 (MQ=255)
      ccGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCgg                                                                                           <  2:31634/100‑1 (MQ=255)
      ccGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCgg                                                                                           >  2:458077/1‑100 (MQ=255)
       cgcgcgTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGc                                                                                          >  2:1840892/1‑100 (MQ=255)
       cgcgcgTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGc                                                                                          <  1:2004139/100‑1 (MQ=255)
       cgcgcgTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGCAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGc                                                                                          <  2:683099/100‑1 (MQ=255)
         cgcgTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCAc                                                                                        <  2:2496205/100‑1 (MQ=255)
         cgcgTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCAc                                                                                        <  1:1338670/100‑1 (MQ=255)
           cgTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTg                                                                                      <  1:3127203/100‑1 (MQ=255)
           cgTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTg                                                                                      >  1:1128946/1‑100 (MQ=255)
           cgTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTg                                                                                      >  2:2418466/1‑100 (MQ=255)
            gTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGc                                                                                     <  1:3211581/100‑1 (MQ=255)
              cTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCtt                                                                                   <  2:3160915/100‑1 (MQ=255)
               tGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCGCTCTCGGCACTGCttt                                                                                  >  1:3763274/1‑100 (MQ=255)
                gTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTc                                                                                 >  1:1977401/1‑100 (MQ=255)
                gTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATGATGGGCGCTCTCTCGGCACTGCTTTc                                                                                 >  2:2275115/1‑100 (MQ=255)
                gTTTGAGGCTCCCGCGCAGTGTTCCGGGATTCTGACTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTc                                                                                 >  2:3691172/1‑100 (MQ=255)
                 tttGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTcc                                                                                >  1:4082681/1‑100 (MQ=255)
                  ttGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCa                                                                               <  1:1122808/100‑1 (MQ=255)
                  ttGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCa                                                                               <  2:2550576/100‑1 (MQ=255)
                   tGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAg                                                                              <  1:3432784/100‑1 (MQ=255)
                    gAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGt                                                                             <  1:3893336/100‑1 (MQ=255)
                    gAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGt                                                                             >  2:328897/1‑100 (MQ=255)
                     aGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGtt                                                                            >  2:2176232/1‑100 (MQ=255)
                      ggCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTg                                                                           >  1:1593205/1‑100 (MQ=255)
                      ggCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTg                                                                           >  1:1727693/1‑100 (MQ=255)
                        cTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGAt                                                                         <  1:781097/100‑1 (MQ=255)
                        cTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGAt                                                                         >  1:2506381/1‑100 (MQ=255)
                        cTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGAt                                                                         <  1:2989265/100‑1 (MQ=255)
                        cTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGAt                                                                         <  1:2695686/100‑1 (MQ=255)
                        cTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGAt                                                                         >  1:2835414/1‑100 (MQ=255)
                         tGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATc                                                                        <  1:3415786/100‑1 (MQ=255)
                         tGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATc                                                                        <  1:2297706/100‑1 (MQ=255)
                         tGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATc                                                                        <  1:3463089/100‑1 (MQ=255)
                          gCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATcg                                                                       <  1:527629/100‑1 (MQ=255)
                              cgcAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATcgacga                                                                   >  2:2118013/1‑100 (MQ=255)
                               gcAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGAt                                                                  <  1:983368/100‑1 (MQ=255)
                                cAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGAtt                                                                 <  2:1947609/100‑1 (MQ=255)
                                    gtTCCGGGATTCTGGCTGGCTGTACGCCGGCGGAGGCGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACt                                                             >  2:1907342/1‑100 (MQ=255)
                                    gtTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACt                                                             >  1:1150115/1‑100 (MQ=255)
                                     ttCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTc                                                            <  1:1871797/100‑1 (MQ=255)
                                      tCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCg                                                           <  1:22903/100‑1 (MQ=255)
                                       ccGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGa                                                          >  2:948121/1‑100 (MQ=255)
                                        cGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGAt                                                         <  2:3998608/100‑1 (MQ=255)
                                         gggATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGAtt                                                        <  2:1390819/100‑1 (MQ=255)
                                         gggATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGAtt                                                        <  2:3215391/100‑1 (MQ=255)
                                           gATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTAc                                                      <  2:134349/100‑1 (MQ=255)
                                           gATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTAc                                                      <  1:1028932/100‑1 (MQ=255)
                                            aTTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACa                                                     >  1:1056849/1‑100 (MQ=255)
                                              tCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAAt                                                   >  1:1238948/1‑100 (MQ=255)
                                              tCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAAt                                                   <  2:3773965/100‑1 (MQ=255)
                                               ctggctggCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGCTTTACTCGATTACAAtc                                                  >  2:485926/1‑99 (MQ=255)
                                                 ggctggctGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGcc                                                >  1:818698/1‑100 (MQ=255)
                                                 ggctggctGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGcc                                                >  2:3051393/1‑100 (MQ=255)
                                                  gctggctgTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCg                                               <  1:79372/100‑1 (MQ=255)
                                                  gctggctgTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCg                                               <  1:875184/100‑1 (MQ=255)
                                                    tggctgTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGAt                                             <  1:3378130/100‑1 (MQ=255)
                                                    tggctgTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGAt                                             <  1:3894019/100‑1 (MQ=255)
                                                    tggctgTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGAt                                             >  1:1799837/1‑100 (MQ=255)
                                                    tggctgTACGCCGGAGGAGGAGAAAGGCCTGCAGGAGTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGAt                                             <  2:614215/100‑1 (MQ=255)
                                                      gctgTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATgg                                           <  2:1217037/100‑1 (MQ=255)
                                                       ctgTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTACTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGc                                          <  2:286136/100‑1 (MQ=255)
                                                           aCGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAAc                                      <  2:3312702/100‑1 (MQ=255)
                                                           aCGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAAc                                      <  1:2315172/100‑1 (MQ=255)
                                                            cGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACa                                     >  2:2306361/1‑100 (MQ=255)
                                                            cGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACa                                     >  1:232325/1‑100 (MQ=255)
                                                               cGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGtt                                  >  1:2694159/1‑100 (MQ=255)
                                                                ggaggaggaGAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTa                                 <  1:3619279/100‑1 (MQ=255)
                                                                  aggaggagAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAgg                               <  1:2523436/100‑1 (MQ=255)
                                                                  aggaggagAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGATTTCCAGTTGATCGACGCTTTACTCGATTACAATGCCGATGGCGAACAGTTAgg                               <  2:3748755/100‑1 (MQ=255)
                                                                    gaggagAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTAACTCGATTACAATGCCGATGGCGAACAGTTAGGTa                             >  2:3941415/1‑100 (MQ=255)
                                                                      ggagAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATGGACGAGTTACTCGATTACAATGCCGACGGCGAACAGTTAGGTaaa                           >  1:3819640/1‑100 (MQ=255)
                                                                      ggagAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTaaa                           >  1:3380533/1‑100 (MQ=255)
                                                                      ggagAAAGGCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTaaa                           >  2:284026/1‑100 (MQ=255)
                                                                             ggCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCg                    <  1:1744970/100‑1 (MQ=255)
                                                                              gCCTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCgg                   >  1:3345104/1‑100 (MQ=255)
                                                                               ccTGCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGc                  >  1:2704794/1‑100 (MQ=255)
                                                                                  gCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGcgac               <  1:9389/100‑1 (MQ=255)
                                                                                  gCAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGcgac               >  1:2328327/1‑100 (MQ=255)
                                                                                   cAGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGcgacg              >  1:2761206/1‑100 (MQ=255)
                                                                                    aGGATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGcgacga             >  2:126917/1‑100 (MQ=255)
                                                                                     ggATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGAt            >  2:648698/1‑100 (MQ=255)
                                                                                     ggATTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGAt            >  2:2003893/1‑100 (MQ=255)
                                                                                      gATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATc           >  2:510796/1‑100 (MQ=255)
                                                                                       aTTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACACTGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCt          >  1:1476243/1‑100 (MQ=255)
                                                                                       aTTATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCt          >  1:587344/1‑100 (MQ=255)
                                                                                        ttATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTg         >  1:496746/1‑100 (MQ=255)
                                                                                         tATGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGa        >  1:2562234/1‑100 (MQ=255)
                                                                                          aTGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGaa       <  1:289496/100‑1 (MQ=255)
                                                                                           tGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAAc      <  1:1307360/100‑1 (MQ=255)
                                                                                           tGGGCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAAc      <  1:707722/100‑1 (MQ=255)
                                                                                           tGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAAc      <  2:1256236/100‑1 (MQ=255)
                                                                                            gggCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACg     <  2:1849504/100‑1 (MQ=255)
                                                                                            gggCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACg     <  2:115005/100‑1 (MQ=255)
                                                                                             ggCGCTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGa    >  1:2426962/1‑100 (MQ=255)
                                                                                              gcgcTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGaa   <  1:2750907/100‑1 (MQ=255)
                                                                                               cgcTCTCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCAGATGGCGACCAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAg  >  1:3790930/1‑100 (MQ=255)
                                                                                                   |                                                                                               
GCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAG  >  NC_000913/3334215‑3334409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A