Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,623,709	T→G	I104S (ATT→AGT)	yhhH →	putative NTF2 fold immunity protein for polymorphic toxin RhsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,623,709	0	T	G	98.7%	502.9 / ‑1.5	148	I104S (ATT→AGT)	yhhH	putative NTF2 fold immunity protein for polymorphic toxin RhsB
Reads supporting (aligned to +/- strand): ref base T (2/0); new base G (72/74); total (74/74)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 148 total aligned reads displayed.

GTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATC  >  NC_000913/3623613‑3623805

gTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGc                                                                                               >  1:593098/1‑100 (MQ=255)

gTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACAGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGc                                                                                               >  2:2908388/1‑100 (MQ=255)

 ttATAGACAGGATGCCGCAGCAGAAGAAAAACCATACGAAATCACTAAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCt                                                                                              <  1:1018287/100‑1 (MQ=255)

   aTGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCtgg                                                                                            >  1:3114154/1‑100 (MQ=255)

    tGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCtggt                                                                                           >  2:29899/1‑100 (MQ=255)

     ggACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCtggtg                                                                                          >  2:2501039/1‑100 (MQ=255)

     ggACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCtggtg                                                                                          >  1:641789/1‑100 (MQ=255)

     ggACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCtggtg                                                                                          >  2:2573696/1‑100 (MQ=255)

       aCAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTggg                                                                                        <  2:3089379/100‑1 (MQ=255)

       aCAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTggg                                                                                        <  1:1377472/100‑1 (MQ=255)

        cAGGATGCCGCAGAAGACGAAAAACCATACGAAATCACTGAGTTTACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTgggg                                                                                       <  1:3749938/100‑1 (MQ=255)

        cAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTgggg                                                                                       <  1:1674693/100‑1 (MQ=255)

          ggATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAGGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGtt                                                                                     >  1:1224627/1‑100 (MQ=255)

          ggATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGtt                                                                                     >  1:1618385/1‑100 (MQ=255)

          ggATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGtt                                                                                     <  1:891588/100‑1 (MQ=255)

          ggATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGtt                                                                                     <  1:891275/100‑1 (MQ=255)

          ggATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGtt                                                                                     >  1:1555643/1‑100 (MQ=255)

          ggATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGtt                                                                                     >  1:764177/1‑100 (MQ=255)

          ggATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGtt                                                                                     >  1:1555946/1‑100 (MQ=255)

            aTGCCGCAGGAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGtttt                                                                                   <  2:2142441/100‑1 (MQ=255)

            aTGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGtttt                                                                                   <  2:2280977/100‑1 (MQ=255)

             ngCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGttttt                                                                                  >  1:2422806/2‑100 (MQ=255)

              gCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTtta                                                                                 <  1:1812823/100‑1 (MQ=255)

               ccGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTttat                                                                                <  1:3325569/100‑1 (MQ=255)

                cGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTttatt                                                                               >  2:2382354/1‑100 (MQ=255)

                 gCAGAAGAAGAAAGACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTttatta                                                                              <  2:169187/100‑1 (MQ=255)

                 gCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTttatta                                                                              <  2:575673/100‑1 (MQ=255)

                 gCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTttatta                                                                              >  2:1934126/1‑100 (MQ=255)

                  cAGAAGGAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGTTTGTTGAAGGTACCATGCACTCAGTTCAAAGTGCTGGTGGTGTTTTtattat                                                                             >  2:2404783/1‑100 (MQ=255)

                  cAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTtattat                                                                             <  1:2369716/100‑1 (MQ=255)

                  cAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTtattat                                                                             <  1:2316743/100‑1 (MQ=255)

                  cAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTtattat                                                                             <  2:2654414/100‑1 (MQ=255)

                   agaagaagaAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATtata                                                                            <  1:970244/100‑1 (MQ=255)

                   agaagaagaAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATtata                                                                            <  1:1711300/100‑1 (MQ=255)

                     aagaagaaAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGa                                                                          >  1:3046336/1‑100 (MQ=255)

                      agaagaaAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGaa                                                                         <  2:1240120/100‑1 (MQ=255)

                       gaagaaAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGaaa                                                                        >  1:2123558/1‑100 (MQ=255)

                       gaagaaAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGaaa                                                                        >  2:3082231/1‑100 (MQ=255)

                       gaagaaAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGaaa                                                                        >  1:1068251/1‑100 (MQ=255)

                       gaagaaAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGATGTTGAAGGTACCATTCACGCAGACCAAAGGGCTCGTGGGGTTTTTATTATAGaaa                                                                        >  2:3785183/1‑100 (MQ=255)

                       gaagaaAAACCATACGAAATCACTGAGTTAACAACAAGATGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGaaa                                                                        >  1:1148045/1‑100 (MQ=255)

                         agaaAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATa                                                                      <  1:1727009/100‑1 (MQ=255)

                         agaaAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATa                                                                      >  1:943952/1‑100 (MQ=255)

                         agaaAAACCATACGAAATCACGGAGTTAACAACAAGTTGGGTCGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATa                                                                      >  1:3479468/1‑100 (MQ=255)

                         agaaAAAACATACGAAATCACTGAGTTAACAACAAGTTAGGTTGTTGAAGGTACCATTCACTCAGAACAAAGTGCTGGTGGGGTTTTTATTATAGAAATa                                                                      <  2:1388300/100‑1 (MQ=255)

                          gaaAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAg                                                                     >  2:669748/1‑100 (MQ=255)

                          gaaAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAg                                                                     >  1:3000628/1‑100 (MQ=255)

                               aCCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCaaa                                                                <  2:3681035/100‑1 (MQ=255)

                                   tACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAatg                                                            <  1:451237/100‑1 (MQ=255)

                                     cGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAatgat                                                          <  2:2955929/100‑1 (MQ=255)

                                     cGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAatgat                                                          >  1:267166/1‑100 (MQ=255)

                                     cGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATCACAGAAATAGGCAAAAatgat                                                          <  1:3483956/100‑1 (MQ=255)

                                        aaTCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATggg                                                       >  1:27081/1‑100 (MQ=255)

                                        aaTCACTGAGTTAACAACAAGATGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTAAAAAAAAAAAAAAAAATGATggg                                                       <  2:1089558/100‑1 (MQ=255)

                                              tGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAAtt                                                 >  1:1618483/1‑100 (MQ=255)

                                               gAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTc                                                >  1:3882692/1‑100 (MQ=255)

                                                aGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCt                                               <  1:2398177/100‑1 (MQ=255)

                                                  ttAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGa                                             >  2:1050071/1‑100 (MQ=255)

                                                   tAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGCATTCTGaa                                            >  1:2902348/1‑100 (MQ=255)

                                                    aacaacAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAAt                                           <  1:4059690/100‑1 (MQ=255)

                                                    aacaacAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAAt                                           <  2:1526509/100‑1 (MQ=255)

                                                     acaacaAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATGATAGAAATAGGCAAAAATGATGGGAGAATTCTGAAtt                                          <  2:3905464/100‑1 (MQ=255)

                                                        acaaGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTg                                       <  2:2175674/100‑1 (MQ=255)

                                                         caaGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTgg                                      <  2:2425659/100‑1 (MQ=255)

                                                         caaGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTgg                                      <  2:1976234/100‑1 (MQ=255)

                                                         caaGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTgg                                      <  1:3910023/100‑1 (MQ=255)

                                                         caaGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTAAAAAAAAAAAAAAAAATGATGGGAGAATTCTGAATTTTgg                                      <  2:416546/100‑1 (MQ=255)

                                                          aaGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGAAAAAATGATGGGAGAATTCTGAATTTTGGc                                     <  2:1692373/100‑1 (MQ=255)

                                                           aGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGcc                                    >  1:1462885/1‑100 (MQ=255)

                                                              tGGGTTGTTGAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACg                                 <  2:3067521/100‑1 (MQ=255)

                                                                  ttgttgAAGGTACCATTCACTCAGACCAAAGTGGTGGTTGGGGTTTGAAAATACAAATAGGCAAAAATGATGGGAGAATTCGGAATTTTGGCCACGGaaa                             <  2:1936847/100‑1 (MQ=255)

                                                                   tgttgCAGGTACCATTCACTCAGACCAAAGTGCTGGCGGGGTTTTTATTATATAAATAGGCAACAATGATGGGAGAATTCTGAATTTTGGCCACGGaaaa                            >  2:3404180/1‑100 (MQ=255)

                                                                   tgttgAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGaaaa                            >  2:3671453/1‑100 (MQ=255)

                                                                    gttgAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAAt                           >  1:1195063/1‑100 (MQ=255)

                                                                     ttgAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATa                          <  2:1050524/100‑1 (MQ=255)

                                                                     ttgAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATa                          <  1:1846562/100‑1 (MQ=255)

                                                                       gAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAg                        >  1:2028115/1‑100 (MQ=255)

                                                                       gAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAg                        >  1:1370351/1‑100 (MQ=255)

                                                                       gAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAg                        >  1:1312536/1‑100 (MQ=255)

                                                                       gAAGGTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAAAAAAAAAAAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAg                        <  2:550648/100‑1 (MQ=255)

                                                                          ggTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTAt                     >  1:1059812/1‑100 (MQ=255)

                                                                          ggTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTAt                     >  2:2588917/1‑100 (MQ=255)

                                                                           gTACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATg                    <  1:1482575/100‑1 (MQ=255)

                                                                            tACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGt                   >  1:1983709/1‑100 (MQ=255)

                                                                            tACCATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGt                   <  1:2603956/100‑1 (MQ=255)

                                                                              ccATTCACTCCGTCCAAAGTGCTGGTGGGGCTTTTATTCTAGAAATACGCAAAAATGATGGGAGTATTATTACTTTTTTGCACTTAAATTATGTATGTaa                 >  2:3564982/1‑100 (MQ=255)

                                                                              ccATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTaa                 <  2:3065971/100‑1 (MQ=255)

                                                                               cATTCACTCAGACCAAAGTGCTGGTGGTGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTATGAATTTTGGCCACGGAAAATAAGTATGTAAg                >  2:1276885/1‑100 (MQ=255)

                                                                               cATTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAg                >  1:3077270/1‑100 (MQ=255)

                                                                                aTTCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAgg               >  1:2640618/1‑100 (MQ=255)

                                                                                  tctcTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGAt             <  2:1187514/97‑1 (MQ=255)

                                                                                  tCACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGAt             <  2:256892/100‑1 (MQ=255)

                                                                                   cACTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATa            <  1:1649094/100‑1 (MQ=255)

                                                                                     cTCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATaag          >  1:3450986/1‑99 (MQ=255)

                                                                                      taaGACGAAAGTGGTGGTGGGGTTTTTATTATAGAAATAGAAAAAAATGCTGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATc         <  2:3935509/98‑1 (MQ=255)

                                                                                      tCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATc         <  1:2076199/100‑1 (MQ=255)

                                                                                      tCAGACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGAAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATc         <  2:2906218/100‑1 (MQ=255)

                                                                                         gACCAAAGTGCTGGTGGGGTTTTTATTATGGAAATAGGCAAAAATGATGGGAGAATTCTGAAGTTTGGCCACGGAAAAGAAGTATGTAAGGATAATCaaa      >  1:3802232/1‑100 (MQ=255)

                                                                                         gACCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCaaa      >  2:2685966/1‑100 (MQ=255)

                                                                                          aCCAAAGTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAAc     <  2:886298/100‑1 (MQ=255)

GTTATGGACAGGATGCCGCAGAAGAAGAAAAACCATACGAAATCACTGAGTTAACAACAAGTTGGGTTGTTGAAGGTACCATTCACTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATC  >  NC_000913/3623613‑3623805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A