Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine tRNA synthetase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,725,1760TG96.3% 546.9 / 4.6 164E48A (GAG→GCG) glyQglycine tRNA synthetase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (3/3);  new base G (74/83);  total (78/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

AGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTA  >  NC_000913/3725081‑3725272
                                                                                               |                                                                                                
agtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGcgcgcg                                                                                              <  2:1281899/100‑1 (MQ=255)
 gtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAgcgcgcgc                                                                                             >  1:1525868/1‑100 (MQ=255)
 gtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAgcgcgcgc                                                                                             >  1:1533781/1‑100 (MQ=255)
   gCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAg                                                                                           >  2:1114789/1‑100 (MQ=255)
   gCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAg                                                                                           >  1:734179/1‑100 (MQ=255)
    cTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCGGCGCGAGCAGa                                                                                          >  2:2937734/1‑100 (MQ=255)
     tGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGAc                                                                                         >  1:1229754/1‑100 (MQ=255)
      gTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACa                                                                                        >  2:2304601/1‑100 (MQ=255)
      gTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACa                                                                                        >  2:2779876/1‑100 (MQ=255)
       tAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAg                                                                                       >  1:3525154/1‑100 (MQ=255)
        aaaCGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAgg                                                                                      <  2:627098/100‑1 (MQ=255)
        aaaCGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAgg                                                                                      <  2:3526296/100‑1 (MQ=255)
            ggTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCAt                                                                                  <  2:1718169/100‑1 (MQ=255)
            ggTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCAt                                                                                  <  2:233535/100‑1 (MQ=255)
               tGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTgg                                                                               >  1:882175/1‑100 (MQ=255)
                ggggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTggg                                                                              <  1:2906890/100‑1 (MQ=255)
                ggggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTggg                                                                              >  1:2765554/1‑100 (MQ=255)
                ggggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTggg                                                                              <  1:3490865/100‑1 (MQ=255)
                 gggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGGCAGGTCATTGGGt                                                                             >  1:2787005/1‑100 (MQ=255)
                 gggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGt                                                                             <  1:3684327/100‑1 (MQ=255)
                  ggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTg                                                                            <  2:1672217/100‑1 (MQ=255)
                  ggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTg                                                                            <  2:718805/100‑1 (MQ=255)
                  ggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTg                                                                            <  2:2272946/100‑1 (MQ=255)
                         gCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGtt                                                                     >  2:2693823/1‑100 (MQ=255)
                           cGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTcc                                                                   <  1:490907/100‑1 (MQ=255)
                           cGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAACCAGCCGCGATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTcc                                                                   >  1:3874041/1‑100 (MQ=255)
                             gaGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCCGCCGCCGTCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCcg                                                                 <  1:2339695/99‑1 (MQ=255)
                               gCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCcgcg                                                               <  2:863527/100‑1 (MQ=255)
                               gCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCcgcg                                                               <  2:519476/100‑1 (MQ=255)
                                 gACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGcc                                                             >  1:244801/1‑100 (MQ=255)
                                 gACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGcc                                                             <  1:2338036/100‑1 (MQ=255)
                                 gACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGcc                                                             >  2:3375916/1‑100 (MQ=255)
                                  aCCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCg                                                            >  2:2885691/1‑100 (MQ=255)
                                    cATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCCGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGAc                                                          >  2:364015/1‑100 (MQ=255)
                                    cATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGan                                                          <  1:300537/100‑2 (MQ=255)
                                     aTCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACt                                                         <  1:3204956/100‑1 (MQ=255)
                                     aTCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCCCGCGTAGACAGGTCAATGGGTGAGAGGTTCCCGCGCCGACt                                                         >  2:3789325/1‑100 (MQ=255)
                                         gtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCa                                                     <  2:1499662/100‑1 (MQ=255)
                                         gtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCa                                                     >  2:1381948/1‑100 (MQ=255)
                                             ggCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTc                                                 >  1:360038/1‑100 (MQ=255)
                                              gCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTcc                                                <  2:1803682/100‑1 (MQ=255)
                                               cgacgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCa                                               >  1:177998/1‑100 (MQ=255)
                                               cgacgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCa                                               <  1:315693/100‑1 (MQ=255)
                                                gacgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCaa                                              >  2:469118/1‑100 (MQ=255)
                                                gacgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCaa                                              >  2:267441/1‑100 (MQ=255)
                                                 acgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAAt                                             >  2:948866/1‑100 (MQ=255)
                                                 acgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAAt                                             >  1:2446339/1‑100 (MQ=255)
                                                 acgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAAt                                             >  1:3025127/1‑100 (MQ=255)
                                                  cgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATg                                            >  1:3437105/1‑100 (MQ=255)
                                                  cgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATg                                            >  2:296880/1‑100 (MQ=255)
                                                  cgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATg                                            >  1:2185530/1‑100 (MQ=255)
                                                  cgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATg                                            <  2:1558809/100‑1 (MQ=255)
                                                    agaAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGt                                          <  1:1801082/100‑1 (MQ=255)
                                                     gaAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGtt                                         <  1:1931369/100‑1 (MQ=255)
                                                      aaGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTg                                        <  1:1015446/100‑1 (MQ=255)
                                                      aaGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTg                                        <  1:1406819/100‑1 (MQ=255)
                                                      aaGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTg                                        >  1:2521170/1‑100 (MQ=255)
                                                       aGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGa                                       >  1:701127/1‑100 (MQ=255)
                                                         gCTGAACATTAGCAGCAGCGATCAGTTCTGTCCCCTGCGCGCTTTGACTGGTCATTGGGTGAGAGGTTACCGCGCCGCCTTCCATGTCCAATGGTTGAAc                                     <  2:434775/100‑1 (MQ=255)
                                                         gCTGAACATGACCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGGCCAAAGGTTGAAc                                     <  2:1227747/100‑1 (MQ=255)
                                                          cTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACa                                    <  1:2818281/100‑1 (MQ=255)
                                                           tGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACaa                                   <  1:2299775/100‑1 (MQ=255)
                                                            gAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAAt                                  >  1:3127688/1‑100 (MQ=255)
                                                            gAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAAt                                  >  1:1923103/1‑100 (MQ=255)
                                                             aaCATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCTGACTTCCATGTCCAATGGTTGAACAATg                                 >  1:3709132/1‑100 (MQ=255)
                                                             aaCATAAGCAGCCGCCATCGGTTCTAGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATg                                 <  2:3792818/100‑1 (MQ=255)
                                                               cATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGt                               <  1:1691621/100‑1 (MQ=255)
                                                                aTAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTg                              >  2:1734227/1‑100 (MQ=255)
                                                                aTAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTg                              >  2:2432235/1‑100 (MQ=255)
                                                                   agcagcCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAg                           <  2:353567/100‑1 (MQ=255)
                                                                   agcagcCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAg                           <  2:2084394/100‑1 (MQ=255)
                                                                   agcagcCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAg                           >  2:3208140/1‑100 (MQ=255)
                                                                    gcggccgcgATCGGTTCTGGCCCCAGGGTGCGCATACAGGGCGTCGGTTGAGAGGGGCCCGCGCCGACTTCCTTGTCCAAGGGTTGTACAATGGTGCGGc                          >  2:2002592/4‑100 (MQ=255)
                                                                    gcagcCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGc                          <  1:441849/100‑1 (MQ=255)
                                                                     cagcCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGcc                         <  1:1944719/100‑1 (MQ=255)
                                                                        ccgccATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTg                      <  1:2841175/100‑1 (MQ=255)
                                                                           ccATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCg                   <  1:2844728/100‑1 (MQ=255)
                                                                            cATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGa                  >  2:1771347/1‑100 (MQ=255)
                                                                             aTCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAg                 >  2:2521072/1‑100 (MQ=255)
                                                                             aTCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAg                 <  2:1075345/100‑1 (MQ=255)
                                                                              tCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGc                <  2:2522447/100‑1 (MQ=255)
                                                                               cGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGcc               <  1:1872697/100‑1 (MQ=255)
                                                                                 gTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCa             <  1:1097660/100‑1 (MQ=255)
                                                                                 gTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCa             >  1:1385456/1‑100 (MQ=255)
                                                                                 gTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCa             >  2:1488804/1‑100 (MQ=255)
                                                                                 gTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGCGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCa             >  2:3896260/1‑100 (MQ=255)
                                                                                  ttatGGCCACAGCGCGCGCAGACACGTCATTGGGGGAGAGGTTCGCGCGCCGACTTCCATGTCCAATGGTCGAACCGTGGTGCAGAGCTGGCGAGCCCAg            <  2:3005393/97‑1 (MQ=255)
                                                                                  ttCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAg            >  2:1440716/1‑100 (MQ=255)
                                                                                   tCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAga           >  1:2195689/1‑99 (MQ=255)
                                                                                   tCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGt           <  1:96404/100‑1 (MQ=255)
                                                                                   tCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGGTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGt           <  1:1537437/100‑1 (MQ=255)
                                                                                     tGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCTCGCCCCGACTTCCATGTCCAATGGTTTAGCAATGGTGCGGCTCTGGCGAGCCCAGtta         >  2:2068478/1‑98 (MQ=255)
                                                                                        ccccCGCGCGCGCAGACAGGTGAGTGGGTGAGGGTGTCCCGCGCCGACTTCCAGGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATcc      <  2:1007765/100‑1 (MQ=255)
                                                                                        ccccAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATcc      <  2:3265147/100‑1 (MQ=255)
                                                                                        ccccAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATcc      >  1:2611205/1‑100 (MQ=255)
                                                                                          ccAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTg    >  1:3394693/1‑100 (MQ=255)
                                                                                          ccAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTg    <  1:3281005/100‑1 (MQ=255)
                                                                                           cAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGt   <  2:2133255/100‑1 (MQ=255)
                                                                                            aGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTa  <  1:2341941/100‑1 (MQ=255)
                                                                                            aGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTa  >  1:3151730/1‑100 (MQ=255)
                                                                                               |                                                                                                
AGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTA  >  NC_000913/3725081‑3725272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A