Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,823,774	A→C	N459H (AAC→CAC)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,823,774	0	A	C	98.8%	590.5 / NA	168	N459H (AAC→CAC)	spoT	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (98/68); total (100/68)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

TGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTC  >  NC_000913/3823677‑3823869

tGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCAc                                                                                               <  2:1137223/100‑1 (MQ=255)

 gACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACt                                                                                              >  2:536800/1‑100 (MQ=255)

 gACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACt                                                                                              <  1:446899/100‑1 (MQ=255)

   ccGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACttt                                                                                            >  1:3830795/1‑100 (MQ=255)

    cGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGATCGCCCGACTGCCGCTTGGCTGCACTTTg                                                                                           >  2:3968282/1‑100 (MQ=255)

     gCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGt                                                                                          <  1:1829179/100‑1 (MQ=255)

     gCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGt                                                                                          >  1:221039/1‑100 (MQ=255)

      ccAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTc                                                                                         >  2:472234/1‑100 (MQ=255)

       cAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCg                                                                                        <  2:2780605/100‑1 (MQ=255)

        aGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCgn                                                                                       <  2:258205/100‑2 (MQ=255)

         gCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGtt                                                                                      >  2:1054703/1‑100 (MQ=255)

          ccTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTa                                                                                     <  1:3528224/100‑1 (MQ=255)

          ccTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTa                                                                                     <  1:1446048/100‑1 (MQ=255)

           cTTACCCGCTGTCGCAGCCGCTTAGTAAAGGTCAAACCGTTGAACTCATTACCGCTCCGGGCGCTCGCCACAGTGCCTCTTGGCTGACCTTTGTCGTTAg                                                                                    >  2:1542977/1‑100 (MQ=255)

           cTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAg                                                                                    <  2:3016752/100‑1 (MQ=255)

            ttACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGc                                                                                   >  1:323171/1‑100 (MQ=255)

            ttACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGc                                                                                   >  1:2507851/1‑100 (MQ=255)

             tACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCt                                                                                  <  1:2720959/100‑1 (MQ=255)

             tACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCt                                                                                  <  2:528581/100‑1 (MQ=255)

              aCCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTc                                                                                 >  1:2813/1‑100 (MQ=255)

               cccGCTGTCGCAGCCGGTTACCAACGGTCAAACCGATGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCCTGGCTGCACTTTGTCGTTAGCTCg                                                                                >  2:3448582/1‑100 (MQ=255)

               cccGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCg                                                                                >  2:657151/1‑100 (MQ=255)

               cccGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCg                                                                                >  1:2271180/1‑100 (MQ=255)

                  gCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGaaa                                                                             >  2:598702/1‑100 (MQ=255)

                       cGCAGCCGCTTACCGGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAgcgcg                                                                        <  2:546829/100‑1 (MQ=255)

                       cGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAgcgcg                                                                        >  1:2409146/1‑100 (MQ=255)

                       cGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAgcgcg                                                                        >  1:2261416/1‑100 (MQ=255)

                        gCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAgcgcgc                                                                       <  2:2372145/100‑1 (MQ=255)

                        gCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAgcgcgc                                                                       >  1:3054156/1‑100 (MQ=255)

                         cAGCCGCTTACCAGCGGTCTAACCGTTGAAAACATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAgcgcgcg                                                                      <  2:3572918/100‑1 (MQ=255)

                         cAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAgcgcgcg                                                                      >  1:1525859/1‑100 (MQ=255)

                         cAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAgcgcgcg                                                                      >  2:164809/1‑100 (MQ=255)

                           gCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGcc                                                                    <  1:3110127/100‑1 (MQ=255)

                            ccGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCa                                                                   <  1:2987759/100‑1 (MQ=255)

                             cGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCaa                                                                  >  2:1528546/1‑100 (MQ=255)

                               cTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCaaaa                                                                >  2:2681159/1‑100 (MQ=255)

                                ttACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAAt                                                               <  2:627931/100‑1 (MQ=255)

                                 tACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAAtt                                                              <  1:2002811/100‑1 (MQ=255)

                                   ccAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCg                                                            >  1:2714829/1‑100 (MQ=255)

                                   ccAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCg                                                            >  2:792511/1‑100 (MQ=255)

                                    cAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCCCTTTGTCGTTAGCTCGACAGCGCGCGCCAAAATTCGt                                                           >  2:2786557/1‑100 (MQ=255)

                                     aGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTc                                                          >  1:460544/1‑100 (MQ=255)

                                     aGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTc                                                          <  2:2508787/100‑1 (MQ=255)

                                     aGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTc                                                          <  1:2095058/100‑1 (MQ=255)

                                     aGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTc                                                          <  1:3038487/100‑1 (MQ=255)

                                      gCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCa                                                         >  1:1782931/1‑100 (MQ=255)

                                      gCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCa                                                         <  1:2325729/100‑1 (MQ=255)

                                        ggTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGt                                                       <  2:3687073/100‑1 (MQ=255)

                                         gTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGtt                                                      >  1:1066629/1‑100 (MQ=255)

                                         gTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGtt                                                      >  1:884583/1‑100 (MQ=255)

                                          tCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTg                                                     <  1:2535580/100‑1 (MQ=255)

                                            aaaCCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCt                                                   <  2:1358666/100‑1 (MQ=255)

                                            aaaCCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCt                                                   <  2:854059/100‑1 (MQ=255)

                                            aaaCCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCt                                                   >  2:3061647/1‑100 (MQ=255)

                                            aaaCCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCt                                                   >  2:2494196/1‑100 (MQ=255)

                                            aaaCCGTTGAAATCATTACCGCTCCGGGAGCTCGCCCGAATGCCGCTTGGCTGCAACTTGTCGTTAGCTCAAAAGAGCGAGCCAAGATTCGTCCGttctt                                                   >  2:3363827/1‑97 (MQ=255)

                                             aaCCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTg                                                  >  1:1019149/1‑100 (MQ=255)

                                             aaCCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTg                                                  >  2:2858305/1‑100 (MQ=255)

                                             aaCCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTg                                                  >  1:3925930/1‑100 (MQ=255)

                                             aaCCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTACACTTTGTCGTTAGCTCGAAAGCGCGCGCCACAATTTGTCAGTTGCTg                                                  >  2:3350277/1‑100 (MQ=255)

                                              aCCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGa                                                 >  2:1060866/1‑100 (MQ=255)

                                              aCCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGa                                                 <  2:1594306/100‑1 (MQ=255)

                                                 gTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGaaaa                                              <  2:3125495/100‑1 (MQ=255)

                                                 gTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGaaaa                                              >  2:2532431/1‑100 (MQ=255)

                                                 gTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGaaaa                                              >  1:3096457/1‑100 (MQ=255)

                                                 gTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGaaaa                                              >  2:2720137/1‑100 (MQ=255)

                                                   tGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAAc                                            >  2:3943547/1‑100 (MQ=255)

                                                    gAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAAcc                                           >  2:268214/1‑100 (MQ=255)

                                                    gAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAAcc                                           <  2:2754219/100‑1 (MQ=255)

                                                    gAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAAcc                                           >  1:2671036/1‑100 (MQ=255)

                                                    gAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAAcc                                           >  1:812312/1‑100 (MQ=255)

                                                       aTCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCa                                        >  2:1797801/1‑100 (MQ=255)

                                                         cATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAg                                      >  2:2023347/1‑100 (MQ=255)

                                                          aTTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGc                                     >  2:2921752/1‑100 (MQ=255)

                                                             aCCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGtg                                  <  2:2526248/100‑1 (MQ=255)

                                                             aCCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGtg                                  <  1:343603/100‑1 (MQ=255)

                                                              ccGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGtga                                 >  2:2493612/1‑100 (MQ=255)

                                                              ccGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGtga                                 >  2:1641056/1‑100 (MQ=255)

                                                              ccGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGtga                                 >  1:3935036/1‑100 (MQ=255)

                                                              ccGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGtga                                 >  1:529167/1‑100 (MQ=255)

                                                              ccGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCGCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGtga                                 >  1:66007/1‑100 (MQ=255)

                                                               cGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGtgat                                >  1:2780934/1‑100 (MQ=255)

                                                               cGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGGCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGtgat                                <  1:2571791/100‑1 (MQ=255)

                                                               cGCTCCGGGCGCACGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGtgat                                >  1:3510488/1‑100 (MQ=255)

                                                                   ccGGGCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGAtt                            >  2:781942/1‑100 (MQ=255)

                                                                    cGGGCGCTGGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTAGTCAGTTGATGAAAAACCTCAAGCGTGATGATTc                           <  2:3149660/100‑1 (MQ=255)

                                                                     gggCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCt                          <  1:3381342/100‑1 (MQ=255)

                                                                     gggCGCTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCt                          >  1:1357987/1‑100 (MQ=255)

                                                                          cTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAg                     >  2:218173/1‑100 (MQ=255)

                                                                          cTCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAg                     >  2:3509199/1‑100 (MQ=255)

                                                                           tCGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGc                    >  1:4011899/1‑100 (MQ=255)

                                                                            cGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAggc                   >  1:1911985/1‑98 (MQ=255)

                                                                            cGCCCGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGcc                   <  2:2273102/100‑1 (MQ=255)

                                                                              cccGAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTg                 <  2:1965050/100‑1 (MQ=255)

                                                                                 gAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGc              >  2:3242321/1‑100 (MQ=255)

                                                                                 gAATGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGc              <  1:2552365/100‑1 (MQ=255)

                                                                                   aTGCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCACGCGTGATGATTCTGTAAGCCTGGGCcg            >  1:2134873/1‑100 (MQ=255)

                                                                                     gCCGCTTGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGTGCcgtc          >  2:1919712/1‑100 (MQ=255)

                                                                                           tGGCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGc    >  2:926030/1‑100 (MQ=255)

                                                                                             gCTGCACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTc  >  1:3152706/1‑100 (MQ=255)

TGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTC  >  NC_000913/3823677‑3823869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A