Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,478,193 T→G E40A (GAA→GCA)  argI ← ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,478,1930TG98.6% 484.9 / ‑1.7 142E40A (GAA→GCA) argIornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (69/70);  total (71/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

Only 100 of 153 total aligned reads displayed.

CACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAAT  >  NC_000913/4478098‑4478289
                                                                                               |                                                                                                
cACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGctgcttc                                                                                              <  1:1621225/100‑1 (MQ=255)
 aCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGctgcttca                                                                                             >  1:2537844/1‑99 (MQ=255)
  cccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGTGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGctgcttctt                                                                                            >  1:1208956/1‑100 (MQ=255)
   ccTGGTCATATGCGGCAACTTCGAACGAGCATCGGGTGCGAGTCGAGTCTTTTTCGAAGATGAGCCCGATGTTTTTACCAGTGAGTTTGGCTGCTTCttt                                                                                           <  1:3796505/100‑1 (MQ=18)
     tGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTAc                                                                                         >  2:1916984/1‑100 (MQ=255)
     tGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTAc                                                                                         >  1:3762188/1‑100 (MQ=255)
      ggTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTAcc                                                                                        >  2:855240/1‑100 (MQ=255)
          atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCtt                                                                                    >  1:1203436/1‑100 (MQ=255)
          atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCtt                                                                                    >  2:3926289/1‑100 (MQ=255)
          atatGCGGCAACTTCGAAAAAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCtt                                                                                    >  2:3653843/1‑100 (MQ=255)
           tatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCttt                                                                                   >  2:3667557/1‑100 (MQ=255)
            atGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCtttt                                                                                  >  2:2189463/1‑100 (MQ=255)
             tGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTc                                                                                 >  1:167335/1‑100 (MQ=255)
             tGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTc                                                                                 <  2:2688024/100‑1 (MQ=255)
               cGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCtt                                                                               >  2:627855/1‑100 (MQ=255)
               cGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCtt                                                                               <  1:1972052/100‑1 (MQ=255)
                ggCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTa                                                                              >  1:498301/1‑100 (MQ=255)
                   aaCTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCg                                                                           <  2:1751998/100‑1 (MQ=255)
                    aCTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCgg                                                                          <  1:1245756/100‑1 (MQ=255)
                     cTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGc                                                                         >  1:1046757/1‑100 (MQ=255)
                     cTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGc                                                                         >  1:1171990/1‑100 (MQ=255)
                     cTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGc                                                                         >  1:1349112/1‑100 (MQ=255)
                      ttCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCt                                                                        <  1:1043497/100‑1 (MQ=255)
                      ttCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCt                                                                        <  1:2791853/100‑1 (MQ=255)
                         gAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTc                                                                     <  1:2180982/100‑1 (MQ=255)
                         gAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTc                                                                     <  2:1403243/100‑1 (MQ=255)
                         gAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTc                                                                     >  1:3454310/1‑100 (MQ=255)
                         gAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTc                                                                     <  2:2011284/100‑1 (MQ=255)
                         gAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTc                                                                     >  1:3167226/1‑100 (MQ=255)
                          aaaGAGCATCGGTTACGAGTCGAGTCTGTTTTGAGGATTAGCGCGATGTTTTTACCAGTTAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCa                                                                    <  2:3821297/100‑1 (MQ=21)
                          aaaGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCa                                                                    <  1:2842367/100‑1 (MQ=255)
                           aaGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTAGGCTGCTTCTATACGGCTTTTCTTATCGGCCTTccg                                                                   >  2:3791107/1‑98 (MQ=21)
                              agCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCtt                                                                >  2:1695788/1‑100 (MQ=255)
                               gCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTc                                                               >  2:1584069/1‑100 (MQ=255)
                                 aTCGGGTACGAGTCGCGTCTTTTTCGAAGAAGAGCGCGATCTTTTAACCAGTGAGTTTGGCTGCTTCTTTACGGCTTTTCTTATCGGCTTTCAGCTTcgc                                                             >  1:3543292/1‑100 (MQ=255)
                                   cGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCgg                                                           <  1:3342446/100‑1 (MQ=255)
                                   cGGGTACGAGTCGAGCGTTTTTCGAAGAGGTGCTCGATGTTTTTACCAGGGAGTTTGGCTGCTTCTTTACCGATTTTCTTATCGGCTTTCAGCTTCGCgg                                                           <  2:2284268/100‑1 (MQ=255)
                                    gggTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGc                                                          <  1:1255628/100‑1 (MQ=255)
                                    gggTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGc                                                          >  2:3060428/1‑100 (MQ=255)
                                     ggTACGAGTCGAGTCTTTTTCGAAGTTGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGAGGCt                                                         >  1:3654689/1‑100 (MQ=37)
                                     ggTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCt                                                         >  2:907731/1‑100 (MQ=255)
                                      gTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTa                                                        <  1:3747051/100‑1 (MQ=255)
                                         cgagtcgagtCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACt                                                     >  2:2885164/1‑100 (MQ=255)
                                          gagtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTg                                                    >  1:1824949/1‑100 (MQ=255)
                                          gagtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTg                                                    >  1:408480/1‑100 (MQ=255)
                                          gagtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTg                                                    <  2:1789143/100‑1 (MQ=255)
                                            gtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTATCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgca                                                  <  1:3844068/100‑1 (MQ=255)
                                            gtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgca                                                  <  2:1467100/100‑1 (MQ=255)
                                              cgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcagc                                                <  1:829740/100‑1 (MQ=255)
                                              cgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcagc                                                >  1:2261711/1‑100 (MQ=255)
                                              cgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcagc                                                <  1:417904/100‑1 (MQ=255)
                                              cgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcagc                                                <  2:3190175/100‑1 (MQ=255)
                                              cgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcagc                                                >  1:2244495/1‑100 (MQ=255)
                                               gagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcagca                                               >  1:156233/1‑100 (MQ=255)
                                               gagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTgcagca                                               >  1:2522421/1‑100 (MQ=255)
                                                  tcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGc                                            <  1:561238/100‑1 (MQ=255)
                                                  tcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGc                                            <  2:516609/100‑1 (MQ=255)
                                                  tcTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGc                                            <  2:3439054/100‑1 (MQ=255)
                                                   cTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCt                                           >  1:2574280/1‑100 (MQ=255)
                                                     ttttCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGt                                         <  2:1346071/100‑1 (MQ=255)
                                                     ttttCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGt                                         >  1:912627/1‑100 (MQ=255)
                                                       ttCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTg                                       <  2:1587758/100‑1 (MQ=255)
                                                       ttCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTg                                       <  2:295920/100‑1 (MQ=255)
                                                       ttCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTg                                       <  1:528084/100‑1 (MQ=255)
                                                       ttCGAAGATGAGCGCGATGTGTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTg                                       <  2:1293316/100‑1 (MQ=255)
                                                        tCGAAGATGAGCGCGATGTTTTTATTTTTTTTTTTTGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGttta                                      >  2:3105374/1‑98 (MQ=255)
                                                         cGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAg                                     <  2:1765706/100‑1 (MQ=255)
                                                          gAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGt                                    >  1:2196085/1‑100 (MQ=255)
                                                          gAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGt                                    >  1:3870418/1‑100 (MQ=255)
                                                            aGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTc                                  <  2:1014850/100‑1 (MQ=255)
                                                            aGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTc                                  <  2:244108/100‑1 (MQ=255)
                                                              aTGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAg                                <  2:555780/100‑1 (MQ=255)
                                                               tGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGc                               <  2:1320901/100‑1 (MQ=255)
                                                               tGAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTGCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGc                               <  1:1168996/100‑1 (MQ=255)
                                                                gAGCGCGATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCCTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCt                              <  1:3468007/100‑1 (MQ=255)
                                                                    gcgATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCg                          <  1:557910/100‑1 (MQ=255)
                                                                    gcgATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCg                          <  1:1696268/100‑1 (MQ=255)
                                                                    gcgATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCg                          <  2:1441813/100‑1 (MQ=255)
                                                                      gATGTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTg                        >  2:655593/1‑100 (MQ=255)
                                                                         gTTTTTACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGaaa                     >  1:3434349/1‑100 (MQ=255)
                                                                            tttACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCg                  >  2:2697469/1‑100 (MQ=255)
                                                                              tACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAg                <  2:2653879/100‑1 (MQ=255)
                                                                              tACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAg                <  1:163501/100‑1 (MQ=255)
                                                                              tACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAg                <  2:3946913/100‑1 (MQ=255)
                                                                              tACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAg                <  1:1729616/100‑1 (MQ=255)
                                                                              tACCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAg                >  1:3354426/1‑100 (MQ=255)
                                                                               aCCAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTTAGCTTCGCGGCTAACTGCAGCAGGCTGTTCAGTTCAGCTGGCGTGAAATCGAGt               <  1:3811113/100‑1 (MQ=255)
                                                                               aCCAGTGAGGTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGt               <  1:3429473/100‑1 (MQ=255)
                                                                                ccAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAgat              >  2:2181184/1‑98 (MQ=255)
                                                                                 cAGTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTaa             >  1:2710994/1‑100 (MQ=255)
                                                                                  ggTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTATTGAGTTCTGCTGGCGTGAAATCGAGTAAt            >  2:3094028/2‑100 (MQ=255)
                                                                                   gTGAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAAtt           >  1:882639/1‑100 (MQ=255)
                                                                                     gAGTTTGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTc         >  2:2135248/1‑100 (MQ=255)
                                                                                        tttGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAgg      <  2:2607477/100‑1 (MQ=255)
                                                                                         ttGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGa     <  2:2898467/100‑1 (MQ=255)
                                                                                          tGGCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGaa    <  2:3529558/100‑1 (MQ=255)
                                                                                           ggCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTTCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGaaa   >  2:790482/1‑100 (MQ=255)
                                                                                           ggCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGaaa   >  1:3686178/1‑100 (MQ=255)
                                                                                           ggCTGCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGaaa   >  1:3173594/1‑100 (MQ=255)
                                                                                            gctgctTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAAt  <  2:1145796/100‑1 (MQ=255)
                                                                                               |                                                                                                
CACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTTACCGCTTTTCTTATCGGCTTTCAGCTTCGCGGCTAACTGCAGCAGGCTGTTGAGTTCAGCTGGCGTGAAATCGAGTAATTTCAGGAAAT  >  NC_000913/4478098‑4478289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A