Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F403 I0 R1	51	156.8	4085907	97.3%	3975587	179.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,745,089	A→C	28.3%	V84G (GTG→GGG)	trmD ←	tRNA m(1)G37 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,745,089	0	A	C	28.3%	174.5 / 12.3	89	V84G (GTG→GGG)	trmD	tRNA m(1)G37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base A (49/11); new base C (3/25); total (52/37)
Fisher's exact test for biased strand distribution p-value = 1.86e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCA  >  NC_000913/2744868‑2745310

ccGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCTACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCCCTTGTTCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCctt                                                                                                                                                                                                                            >  1:13443/1‑227 (MQ=255)

ccGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCCCCttt                                                                                                                                                                                                                            >  1:1424077/1‑227 (MQ=255)

 cGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTg                                                                                                                                                                                                                           <  2:1396849/227‑1 (MQ=255)

     aTAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCCGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACGGCTAAA‑TCACCTTTG‑CGCCt                                                                                                                                                                                                                     >  1:1614867/1‑228 (MQ=255)

     aTAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCt                                                                                                                                                                                                                     >  2:1730034/1‑228 (MQ=255)

         aCCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTTAc                                                                                                                                                                                                                 >  1:1721867/1‑228 (MQ=255)

                          aTCAGCGTCATTGCTGTTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCCCCTTTG‑CGCCTTCACCCGCCGCGGCTTTtgc                                                                                                                                                                                                 <  1:1639973/227‑1 (MQ=255)

                            cgccttCATTGCTGTAAACCCCCCACCACTGAAACCGTAATCGCCGTTTGCCCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGCCCGCACCCCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTtgctgc                                                                                                                                                                                              <  2:1612011/223‑1 (MQ=255)

                               cGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCTGCTTTTGCTGCAt                                                                                                                                                                                            >  1:709914/1‑227 (MQ=255)

                                 tCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTTTGGTG‑ACAGATAAA‑TCACCTTTC‑CGCCTTCACCCGCCCGGGCCTTTGCTGCATGaa                                                                                                                                                                                         >  1:471755/1‑228 (MQ=255)

                                      gCTGGTAACTCCCCCCCACTGAAACCGTACCCGCCGTTTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCCCCTTTG‑CGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCg                                                                                                                                                                                    <  2:1142726/228‑1 (MQ=255)

                                      cttgttAACTCCCCACCAGTGAAAACGTAATCCCCAATTGACCATTTTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATCCCTTC‑GTAGCGACCGCCCACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCCCCTTTG‑CGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCg                                                                                                                                                                                    <  2:409375/223‑1 (MQ=255)

                                       ctggaaccccaccaccacGGAGAACGTAATCGCCAATTGCCCTTTCTTCGTCATTTTCGGTTTGGATCCCGCGTTCATCTATACCTTC‑GTAGCGACCCCACACCGGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCCCCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGt                                                                                                                                                                                   <  2:1175700/219‑1 (MQ=255)

                                             accacccaccacGGAAACCGTAACGCCCGATGAGCCGTGCTTCGACAATGCGGGTTGGGACCCGGCGCTCATCTATCCCTTC‑GAGGCGACCGCACACCAGAATCAATGTTTGAT‑TCGTTGCCAGGTCGCTGACGCCCGCTTGACAAAGCTGGC‑GTCCCTGGGGTG‑ACAGATAAA‑CCCCCTTGG‑CGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑Ca                                                                                                                                                                            <  2:285510/223‑1 (MQ=255)

                                              cTCACCTCCACGTCGATCGTACTCCCATATTGCCCTTTCATCATCATTTTCGGTTTGGATCACGCGCCCATCTTACCCTTC‑GTAGCGCCCGCACCCCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCCCCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑Caa                                                                                                                                                                           <  2:1421527/228‑1 (MQ=255)

                                                 accaccacTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGGTGTT‑ACAGATAAA‑TCCCCTTTG‑CGCCTTTGCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAg                                                                                                                                                                          >  2:1286707/1‑226 (MQ=255)

                                                     ccacTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTg                                                                                                                                                                     >  1:1194286/1‑227 (MQ=255)

                                                      cacTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTTTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGTTGCATGAAGGGCGTCCCGCCAAGGGtgcc                                                                                                                                                                    >  2:1244980/1‑225 (MQ=255)

                                                       ggactccggctAAGCGCCGTATGCCCCTTCTTCCTCAATTTGGGTTTGGAACACCGCCTTCTTTATTCCTTC‑GGAGCCACCGCACCCCAAAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGAACAAGCTTGC‑CGCCCTGTGGTG‑AAAGAAAAA‑TCCCCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCa                                                                                                                                                                   <  2:1564914/217‑1 (MQ=255)

                                                       ctggaaaccGTAACCGCCGTTTGCCCATTCTTCGTCATTTCCGGTTTGGATCCCGCCCTCATCTATCCCTTC‑GTAGCGCCCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTCCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCCCCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCa                                                                                                                                                                   <  2:1189086/219‑1 (MQ=255)

                                                       acTGAACACGTAATCGCTGATTGACCATTCTTCGTCAATTTCGGTTTGGATCCCGCGCTCATTTATACCTTC‑GTAGCGACCGCACCCCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCCCCTTTG‑CGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCa                                                                                                                                                                   <  2:13416/227‑1 (MQ=255)

                                                        ttGAGAACGTAACCGCCGTTTGCCCTTTCTTCGTCAATTTCGGTTTGGATCACGCGCTTATCTATCCCTTC‑GTAGCGCCCGCACCCCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCCCCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCa                                                                                                                                                                   <  2:376726/225‑1 (MQ=255)

                                                           agaACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGc                                                                                                                                                                    >  1:377348/1‑222 (MQ=255)

                                                                       ccGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CCAGGGTTGCACCATCATTATCATctcc                                                                                                                                                  >  2:1131545/1‑228 (MQ=255)

                                                                          aTTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTC‑CGCCTTCCCCCGCCCCGGCTTTTGCTGCAGGAATGGCTTCCCG‑CAAGGGTTGCACCACCCTTatccggctcct                                                                                                                                                >  1:1567696/1‑217 (MQ=255)

                                                                              ccctttcctcGACATTTTCGGTTGGACACACGCGTCTATCTTTCCTTTC‑GTCGCGCCCGCACCCCAAAATCAATTTTTGAT‑GCTTTGCCAGTTCTCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCCCCTTTG‑CGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCCCCATCATTAACATCCCCGGTccgc                                                                                                                                           <  2:631998/224‑1 (MQ=255)

                                                                                      gctaaagtgcgggTGTGAATCGCGCGCACACCTAGCCGTAC‑GGAGCGACCGCACCACAAAATCAATTTGTGAT‑TGTTTCCCAGTCCGTTGCCGCCCGTTGGAACAAGCTTGC‑GTCCCTGGGGTA‑CCAGAAAAA‑TCCCCTTTG‑CGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTa                                                                                                                                    <  2:237871/216‑1 (MQ=255)

                                                                                       cGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGc                                                                                                                                                                    >  1:574177/1‑194 (MQ=255)

                                                                                        gTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCa                                                                                                                                                                   <  2:573241/194‑1 (MQ=255)

                                                                                             tttCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGa                                                                                                                             >  1:1175105/1‑227 (MQ=255)

                                                                                              ttCGGTTTGGATCTCGCGCTCACTTAT‑CCTTCTGTGCCACCCCCACCCAAGATTCATTTTTTGAT‑TCTTTGCCAGTTCGCTAACGCCCGTTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAC‑TCCCCTTTG‑CGCCTCCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGAt                                                                                                                            <  2:1173000/227‑1 (MQ=255)

                                                                                                   tttGGCTCACGTTCTTATCTATCCCTGC‑ATAGCCACCGCACCCCAGTATAAATTTTTGAT‑TCGTTGCCGTTTCGCTACCGCCGTTTTGATCAAGCTGGC‑G‑CCCCGTGGGGTAAAGATAAA‑CCCCCTTTG‑CCCTTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGCCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCa                                                                                                                      <  2:1875227/228‑1 (MQ=255)

                                                                                                   tttGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGCCGATCGGcc                                                                                                                       >  1:1397668/1‑227 (MQ=255)

                                                                                                            cgcgCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGGCCacggtacggt                                                                                                             >  1:519729/1‑228 (MQ=255)

                                                                                                                  cATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCCCCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATTGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCGGCCGTAAGGACGATCGTCCACGGCACGGTGCCgg                                                                                                        >  1:507567/1‑227 (MQ=255)

                                                                                                                       ataCCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACTGTGCCGGTCATGc                                                                                                  >  1:34191/1‑228 (MQ=255)

                                                                                                                             tC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTTC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCACCATTAACATCCCCGGTccgacgc                                                                                                                                        >  1:691603/1‑184 (MQ=255)

                                                                                                                             tC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCTGCCGCGGCTTTTGCTGCATGCATGGCGTCCCC‑CAAGGGTTGCACCCTCCTTCACATCCCCGGTCCGCCGCCGCAAGGACGATCGTCCCCGGTACGGCGCCGGCCATGCGTCaa                                                                                             >  1:545294/1‑227 (MQ=255)

                                                                                                                             tC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGaa                                                                                             >  2:1183121/1‑227 (MQ=255)

                                                                                                                             tC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGaa                                                                                             >  2:1967017/1‑227 (MQ=255)

                                                                                                                             tC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGGGaa                                                                                             >  1:584859/1‑227 (MQ=255)

                                                                                                                                gTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCacggt                                                                                                                  >  1:1356767/1‑204 (MQ=255)

                                                                                                                                 tagcacccGCCCACCAGAATCATTTTTTGAT‑TGTTTGCCAGTTCGCTGACGCCGGCTTGATCAAGTTGGC‑CTCCCTGTGGTG‑ACAGAAAAA‑CCCCTTTGG‑CGCTTCCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCacggta                                                                                                                 <  2:1354329/198‑1 (MQ=255)

                                                                                                                                     gACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTCCGGTGCCGGTCATGCGGTAAGTcgcg                                                                                       >  2:1420369/1‑226 (MQ=255)

                                                                                                                                     gACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTcgcg                                                                                       >  2:764065/1‑226 (MQ=255)

                                                                                                                                      aCCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTcgcg                                                                                       >  1:570627/1‑225 (MQ=255)

                                                                                                                                      aCCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGtt                                                                                                                                                                      >  2:1151820/1‑146 (MQ=255)

                                                                                                                                      aCCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTcgcg                                                                                       >  2:1021580/1‑225 (MQ=255)

                                                                                                                                       ccGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTGGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTg                                                                                                                                                                     <  1:1153887/146‑1 (MQ=255)

                                                                                                                                          caccacAGTATCTATTTTGGATGTCGTGCCCATTCCTCTGTCGCCCGTTTGAACAAGCTGGC‑TTCCTGGTGGTG‑ACAGAAAAA‑CCCCTTTTG‑CCCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACt                                                                                 <  2:1395163/223‑1 (MQ=255)

                                                                                                                                          cacaCCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGCGGACTc                                                                                >  1:181539/1‑228 (MQ=255)

                                                                                                                                                    tCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTgg                                                                       >  1:1240688/1‑227 (MQ=255)

                                                                                                                                                      gaTTTTGGAT‑CCTGTGCCAGTCCGCTGCCGCCCGGTTGCCCCAGCTCTC‑CCCCGTGTGGTG‑ACACAAAAA‑CCCCCTTGG‑CGCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATg                                                                    <  2:506737/227‑1 (MQ=255)

                                                                                                                                                              aT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGc                                                                                                                                                                    >  2:162721/1‑124 (MQ=255)

                                                                                                                                                               t‑tCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCa                                                                                                                                                                   <  1:163005/124‑1 (MQ=255)

                                                                                                                                                                                tGACGCCCGTTTGA‑CAAGCTTGCGGTCCTTGTGGTG‑AAAGATAAC‑TCCCTTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGcc                                            <  1:1367525/227‑1 (MQ=255)

                                                                                                                                                                                   cGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGACGTCCCGAGGACTCCAGCCATGGATGCTCAGCAGGCCATTTTTAACTGc                                             >  1:1660017/1‑223 (MQ=255)

                                                                                                                                                                                   cGCCCGCTTGATCAAGCTTGC‑GTCCCTGGGGTG‑ACAGATAAA‑CCACCTTGG‑CGCCTGCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGc                                        <  1:1209646/228‑1 (MQ=255)

                                                                                                                                                                                     cccGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCTTGCGTGAAGTCGCGAGGACTCCAGCTCTGGATCCTCAGCAGGCCATTTTTAACTGCCCGGCCa                                      >  2:889913/1‑228 (MQ=255)

                                                                                                                                                                                       cGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCCCAGCAGGCCCTTTTTAACTGCCCGGcccg                                     >  1:1599018/1‑225 (MQ=255)

                                                                                                                                                                                       cGCTTCATCCAGCTTGC‑‑TCCCTG‑GGTGTAACGATAAACTCCCTTTGG‑CCTCTTCCCCCGCCGCGGCTTTTGCTGCATGACTGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGt                                    <  2:89186/228‑1 (MQ=255)

                                                                                                                                                                                              tCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCacggta                                                                                                                 >  1:1449849/1‑144 (MQ=255)

                                                                                                                                                                                              acaggcttgc‑ttCCGTTGGGTA‑ACAGAAAAC‑TCCCCTTCG‑CGCCTTCCCCCGCCGTGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCg                             <  2:181225/224‑1 (MQ=255)

                                                                                                                                                                                               cAAGGTTGG‑CTCCGTGTGGTG‑ACAGAAAAA‑CCCCCTTTC‑CGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCCCCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCacggtac                                                                                                                <  2:1447257/144‑1 (MQ=255)

                                                                                                                                                                                                agccTTGC‑TCCCTTGTGGTG‑AAAGAAAAA‑CCCCTTTGG‑CCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTa                           <  1:906587/225‑1 (MQ=255)

                                                                                                                                                                                                   cTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGCTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGCGAAGTCGCGAGGACTCCAGct                                                                           >  1:295569/1‑177 (MQ=255)

                                                                                                                                                                                                       c‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCCTTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGt                     >  1:1908847/1‑227 (MQ=255)

                                                                                                                                                                                                       c‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTa                    >  2:416874/1‑228 (MQ=255)

                                                                                                                                                                                                       c‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTCCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGGCCGGCCAGTTACCCCGTCATCGGTa                    >  1:1869843/1‑228 (MQ=255)

                                                                                                                                                                                                         gTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGt                     >  1:1022609/1‑226 (MQ=255)

                                                                                                                                                                                                             cTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCg                                                                                                                                                                               >  1:1281728/1‑69 (MQ=255)

                                                                                                                                                                                                             cTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTg                >  1:186765/1‑227 (MQ=255)

                                                                                                                                                                                                             cTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGCTACTTg                >  2:529419/1‑227 (MQ=255)

                                                                                                                                                                                                              tgtgGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑c                                                                                                                                                                             <  2:1279433/69‑1 (MQ=255)

                                                                                                                                                                                                              tgtgGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTgcg              >  2:1797750/1‑228 (MQ=255)

                                                                                                                                                                                                               gtggta‑ACAGATAAA‑TCACCTTGG‑CGCCTCCCCCCGCCGCGGCTTTGGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACGTCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTgcg              <  1:1422917/227‑1 (MQ=255)

                                                                                                                                                                                                                gtgtgtgaaaaTAAA‑TCCCCTTTGCCACCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTgcg              <  2:820045/218‑1 (MQ=255)

                                                                                                                                                                                                                 ggtg‑ACAGATAAA‑TCCCTTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCgg           <  1:446376/228‑1 (MQ=255)

                                                                                                                                                                                                                    g‑ACATATAGA‑TCACCTTGG‑CGCTTTCGCCCGCCGCGGCTTTTGCTGCATGAAGGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGGAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGaa         <  1:1133579/227‑1 (MQ=255)

                                                                                                                                                                                                                     aaCAGATAAT‑TC‑CCTTTGCCGCTTTCCCCCCCCGCGGCTTTTGCTGCAGGACGGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGaa         <  2:1905462/226‑1 (MQ=255)

                                                                                                                                                                                                                      aCAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAgg                                                                                                                                                                         >  2:1430389/1‑66 (MQ=255)

                                                                                                                                                                                                                      aCAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGa                                                                                      >  2:281563/1‑149 (MQ=255)

                                                                                                                                                                                                                      aCAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACa       >  2:731845/1‑228 (MQ=255)

                                                                                                                                                                                                                       caaaaaaa‑TCCCCTTGG‑CGCTTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACCTCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACa       <  1:103041/222‑1 (MQ=255)

                                                                                                                                                                                                                       cAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAggg                                                                                                                                                                        <  1:1432954/66‑1 (MQ=255)

                                                                                                                                                                                                                       cAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAg                                                                                     <  1:282044/149‑1 (MQ=255)

                                                                                                                                                                                                                        aGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACCtt     >  1:1169652/1‑228 (MQ=255)

                                                                                                                                                                                                                        aGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACAGCCCGGCCAGTTACCCCGTAATCGGGAATTTCGCGGAGCAtt     >  2:1446474/1‑228 (MQ=255)

                                                                                                                                                                                                                           tAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGCAATTGCGCGGAACATTTCa  >  1:1558546/1‑228 (MQ=255)

CCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAATCAATTTTTGAT‑TCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGC‑GTCCCTGTGGTG‑ACAGATAAA‑TCACCTTTG‑CGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCG‑CAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCA  >  NC_000913/2744868‑2745310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 6 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A