Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F412 I2 R1 70 105.0 5244496 94.3% 4945559 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,917,011 T→C N122S (AAC→AGC)  atpD ← F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,917,0110TC89.6% 291.7 / 19.7 106N122S (AAC→AGC) atpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base T (5/6);  new base C (45/50);  total (50/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 106 total aligned reads displayed.

ACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTC  >  NC_000913/3916915‑3917109
                                                                                                |                                                                                                  
acacCCGCACCACCGAACAGACCAACTTGACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGATAGCTTg                                                                                                 <  2:2532296/100‑1 (MQ=255)
  acCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGAc                                                                                               <  1:1780849/100‑1 (MQ=255)
   cccGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACa                                                                                              >  1:963777/1‑100 (MQ=255)
   cccGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACa                                                                                              >  1:2589791/1‑100 (MQ=255)
   cccGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACa                                                                                              >  2:1090876/1‑100 (MQ=255)
   cccGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACa                                                                                              >  2:2377573/1‑100 (MQ=255)
      gCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGct                                                                                           >  1:822211/1‑100 (MQ=255)
      gCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGct                                                                                           >  1:245265/1‑100 (MQ=255)
       caccacCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGctc                                                                                          <  2:709999/100‑1 (MQ=255)
        nccaccGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGctct                                                                                         >  1:169837/2‑100 (MQ=255)
        accaccGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGctct                                                                                         <  1:775303/100‑1 (MQ=255)
          caccGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTc                                                                                       >  1:2032492/1‑100 (MQ=255)
          caccGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTc                                                                                       <  1:2354409/100‑1 (MQ=255)
          caccGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTc                                                                                       <  2:2023565/100‑1 (MQ=255)
          caacGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTc                                                                                       <  2:1900877/97‑1 (MQ=255)
             cGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTa                                                                                    <  1:1698556/100‑1 (MQ=255)
               aaCAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAgg                                                                                  >  1:1054900/1‑100 (MQ=255)
                 cAGACCAACTTTACTGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCGTGACAGCTCTTCGTAGGaa                                                                                <  2:2375330/100‑1 (MQ=255)
                 cAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGaa                                                                                <  2:395008/100‑1 (MQ=255)
                  aGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAg                                                                               <  2:2546223/100‑1 (MQ=255)
                   gACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAgg                                                                              <  2:1742582/100‑1 (MQ=255)
                   gACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAgg                                                                              >  1:2287836/1‑100 (MQ=255)
                   gACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTACGAAgg                                                                              <  2:2285293/100‑1 (MQ=255)
                     ccAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGtg                                                                            >  2:2122278/1‑100 (MQ=255)
                         cTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGtgctgc                                                                        >  1:647095/1‑100 (MQ=255)
                          tttACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTgcg                                                                       <  1:877747/100‑1 (MQ=255)
                           ttACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTgcgc                                                                      <  1:1300750/100‑1 (MQ=255)
                            tACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGcgcg                                                                     <  2:1349470/100‑1 (MQ=255)
                             aCCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCgg                                                                    <  1:2446904/100‑1 (MQ=255)
                               cGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTg                                                                  <  2:1762426/100‑1 (MQ=255)
                                gCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGa                                                                 >  1:921561/1‑100 (MQ=255)
                                 cccTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGaa                                                                <  2:2257637/100‑1 (MQ=255)
                                  ccTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAAt                                                               <  2:1787167/100‑1 (MQ=255)
                                   cTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATc                                                              <  1:783790/100‑1 (MQ=255)
                                     tAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGc                                                            <  2:1005665/100‑1 (MQ=255)
                                     tAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGc                                                            <  2:659318/100‑1 (MQ=255)
                                      aGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAACCGcc                                                           >  1:2368456/1‑100 (MQ=255)
                                        cGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCa                                                         >  1:1144667/1‑100 (MQ=255)
                                           aCGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACg                                                      <  1:1639814/100‑1 (MQ=255)
                                            cGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGc                                                     <  2:1108549/100‑1 (MQ=255)
                                            cGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGc                                                     <  1:829101/100‑1 (MQ=255)
                                             ggACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGct                                                    <  2:634042/100‑1 (MQ=255)
                                              gACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGctc                                                   >  1:2189418/1‑100 (MQ=255)
                                              gACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGctc                                                   >  1:1174614/1‑100 (MQ=255)
                                               acacATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGctct                                                  <  1:1325815/100‑1 (MQ=255)
                                                cacaTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCtctt                                                 >  1:1418975/1‑100 (MQ=255)
                                                 acgtCAGGTCGATAACATCGATACCGGTTTCCAGCATTTCGTGTACGCTGGCCAGCTCGTAGTAGGAAGGTGATCAGCGGTCAATCGCCCAACGCtcttc                                                <  2:158215/97‑1 (MQ=255)
                                                  naTCAGGTCGATAACTTTGATACCGGGTTCCAGCAGTTCCTGAGAGCTTGACGTCTCGTCGTAGGAAGGGGCTGCGCGGTGAATCGCCCAGCGCgcttgt                                               >  1:2114302/2‑98 (MQ=255)
                                                  caTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCtcttct                                               >  1:762516/1‑100 (MQ=255)
                                                  caTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCtcttct                                               >  1:1199443/1‑100 (MQ=255)
                                                  caTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCtcttct                                               >  1:1883082/1‑100 (MQ=255)
                                                  caTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCtcttct                                               >  2:2218007/1‑100 (MQ=255)
                                                  caTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCtcttct                                               >  1:2184731/1‑100 (MQ=255)
                                                  caTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCtcttcn                                               <  1:2105243/100‑2 (MQ=255)
                                                    tCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCttcttc                                             <  1:1942849/100‑1 (MQ=255)
                                                       ggTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCAcc                                          <  1:1679275/100‑1 (MQ=255)
                                                        gTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGCGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCg                                         >  1:2641012/1‑100 (MQ=255)
                                                        gTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCg                                         <  2:2542985/100‑1 (MQ=255)
                                                          cGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGAt                                       >  1:1676474/1‑100 (MQ=255)
                                                          cGATAACTTTGATACCGGTTTCCACCAGTACCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGTCCAACGCTCTTCTTCACCGAt                                       >  1:2465047/1‑100 (MQ=255)
                                                          cGAAAACTTTGATACCGGTTTCCCGCAGTTCCTGAGAGCTTGACAGCTCTTAGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGAt                                       >  2:1283/1‑100 (MQ=255)
                                                           gATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGAtc                                      >  2:1369025/1‑100 (MQ=255)
                                                            aTAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGAtct                                     <  1:1345408/100‑1 (MQ=255)
                                                              aaCTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCg                                   <  2:1108250/100‑1 (MQ=255)
                                                               aCTTTGACACCGGTTTGCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGGGCATCGCCCAACGCTCTTCTTCACCGATCTCGc                                  <  2:521313/100‑1 (MQ=255)
                                                                cTTTGATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGcc                                 >  2:263429/1‑100 (MQ=255)
                                                                    gATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTc                             >  1:2269776/1‑100 (MQ=255)
                                                                    gATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTc                             >  1:2214448/1‑100 (MQ=255)
                                                                    gATACCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCACCTTTc                             >  1:617641/1‑100 (MQ=255)
                                                                      tACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCAt                           >  1:415084/1‑100 (MQ=255)
                                                                       aCCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATg                          <  2:1639256/100‑1 (MQ=255)
                                                                       aCCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATg                          >  2:48586/1‑100 (MQ=255)
                                                                       aCCGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATg                          <  1:146944/100‑1 (MQ=255)
                                                                        ccGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGt                         >  1:581104/1‑100 (MQ=255)
                                                                        ccGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGGTCTTCATCACCGATCTCGCCTTTCATGt                         >  1:2402038/1‑100 (MQ=255)
                                                                        ccGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGt                         <  2:2128882/100‑1 (MQ=255)
                                                                        ccGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACACTCTTCTTCACCGATCTCGCCTTTCATGt                         >  1:2413370/1‑100 (MQ=255)
                                                                         cGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTc                        <  1:1221270/100‑1 (MQ=255)
                                                                         cGGTTTCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTc                        <  2:2572837/100‑1 (MQ=255)
                                                                            tttCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGAc                     <  2:2471100/100‑1 (MQ=255)
                                                                             ttCCAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGAcc                    <  2:1038500/100‑1 (MQ=255)
                                                                               ccAGCAGTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCgg                  >  2:157520/1‑100 (MQ=255)
                                                                                cagcagTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGt                 <  2:807733/100‑1 (MQ=255)
                                                                                cagcagTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGt                 >  1:336811/1‑100 (MQ=255)
                                                                                 agcagTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGtt                <  2:747930/100‑1 (MQ=255)
                                                                                     gTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCAcc            >  2:493865/1‑100 (MQ=255)
                                                                                     gTTCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCAcc            >  1:2031731/1‑100 (MQ=255)
                                                                                      ttCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCAccc           <  1:1547421/100‑1 (MQ=255)
                                                                                      ttCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCAccc           <  2:1527829/100‑1 (MQ=255)
                                                                                      ttCCTGAGACCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCAccc           <  2:2247726/100‑1 (MQ=255)
                                                                                       tCCTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCa          >  1:2199602/1‑100 (MQ=255)
                                                                                        ccTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAg         <  1:193340/100‑1 (MQ=255)
                                                                                        ccTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAg         <  1:2337246/100‑1 (MQ=255)
                                                                                         cTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGt        >  1:1622604/1‑100 (MQ=255)
                                                                                         cTGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGt        >  2:2416466/1‑100 (MQ=255)
                                                                                          tGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTa       >  1:926019/1‑100 (MQ=255)
                                                                                          tGAGAGCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTa       >  1:2432797/1‑100 (MQ=255)
                                                                                            agagCTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACg     <  2:2267663/100‑1 (MQ=255)
                                                                                               gTTTGACCGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCACCGCTCTTCTTCACCGCTCTCGCCTTTCATGTCGACCGGTTCACCCAGTCCGTTc  <  2:2592982/100‑1 (MQ=255)
                                                                                               gTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTc  <  2:569299/100‑1 (MQ=255)
                                                                                                |                                                                                                  
ACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTC  >  NC_000913/3916915‑3917109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A