Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F406 I1 R1	19	66.1	1879446	94.7%	1779835	168.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,798,659	(T)_8→7	coding (654/1074 nt)	waaU ←	lipopolysaccharide core biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,798,652	0	T	.	94.6%	139.8 / 3.2	37	coding (661/1074 nt)	waaU	lipopolysaccharide core biosynthesis
Reads supporting (aligned to +/- strand): ref base T (1/1); new base . (20/15); total (21/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

ACAGAAATGACAAAATCACTATACTTGGTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCATCGTGGGGATGGTAAAATGAATAGTAACGCTTATACCAATGATCA  >  NC_000913/3798456‑3798875

aCAGAAATGACAAAATCACTATACTTGGTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTaa                                                                                                                                                                                                                  >  1:777779/1‑212 (MQ=255)

aCAGAAATGACAAAATCACTATACTTGGTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTaa                                                                                                                                                                                                                  <  2:777779/212‑1 (MQ=255)

                          ggTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTc                                                                                                                                                            >  2:624850/1‑239 (MQ=255)

                          ggTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑‑TTTTTTGCACCTAATGGa                                                                                                                                                                                                              >  2:619442/1‑188 (MQ=255)

                          ggTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑‑TTTTTTGCACCTAATGGa                                                                                                                                                                                                              <  1:619442/188‑1 (MQ=255)

                                                 aCTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTACTAATGa                                                                                                                                                                                                    >  2:526218/1‑176 (MQ=255)

                                                 aCTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGa                                                                                                                                                                                                    <  1:526218/176‑1 (MQ=255)

                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTaataat                                                                                                                                                                                                      >  2:915810/1‑173 (MQ=255)

                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTaataat                                                                                                                                                                                                      >  2:35187/1‑173 (MQ=255)

                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTaataat                                                                                                                                                                                                      <  1:915810/173‑1 (MQ=255)

                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTaataat                                                                                                                                                                                                      <  1:35187/173‑1 (MQ=255)

                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTAtt                                                                                                                                                                                               >  1:275523/1‑180 (MQ=255)

                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTAtt                                                                                                                                                                                               <  2:275523/180‑1 (MQ=255)

                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCCATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTc                                                                                                                                    >  2:896233/1‑239 (MQ=255)

                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAAt                                                                                                                                           >  1:893502/1‑232 (MQ=255)

                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAAt                                                                                                                                           <  2:893502/232‑1 (MQ=255)

                                                     atcaAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGa                                                                                                                                                                                                              >  2:808920/1‑162 (MQ=255)

                                                     atcaAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGa                                                                                                                                                                                                              <  1:808920/162‑1 (MQ=255)

                                                                       gATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTAcc                                                                                                               <  1:624850/239‑1 (MQ=255)

                                                                        aTTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTAcc                                                                                                               >  2:147311/1‑238 (MQ=255)

                                                                                    ggtattgGCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAata                                                                                                  <  2:187845/239‑1 (MQ=255)

                                                                                          gTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTaa                                                                                                                                                                                                                  >  2:242976/1‑121 (MQ=255)

                                                                                          gTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTaa                                                                                                                                                                                                                  <  1:242976/121‑1 (MQ=255)

                                                                                                       gCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCt                                                                                                                                                                                                                    >  1:631206/1‑106 (MQ=255)

                                                                                                       gCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCt                                                                                                                                                                                                                    <  2:631206/106‑1 (MQ=255)

                                                                                                       gCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTg                                                                               <  1:147311/239‑1 (MQ=255)

                                                                                                                            tCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATaaa                                                                                                    <  2:415439/197‑1 (MQ=255)

                                                                                                                            tCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATaaa                                                                                                    >  1:415439/1‑197 (MQ=255)

                                                                                                                                               tgtTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTaa                                                                                                                                                                                                                  <  2:626856/68‑1 (MQ=255)

                                                                                                                                               tgtTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTaa                                                                                                                                                                                                                  >  1:626856/1‑68 (MQ=255)

                                                                                                                                                       cttcttGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCATCGTGGGGATGGTaaa                               >  1:926789/1‑239 (MQ=255)

                                                                                                                                                             gATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCATCGTGGGGATGGTAAAATGAAt                         >  2:56866/1‑239 (MQ=255)

                                                                                                                                                                  ataACTTTTATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTTATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCATCGTGGGGATGGTAAAATGAATAGTa                     >  2:849091/1‑238 (MQ=255)

                                                                                                                                                                         ttATTTGCTCAAACGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCATCGTGGGGATGGTAAAATGAATAGTAACGCTtat             >  1:105655/1‑239 (MQ=255)

                                                                                                                                                                                    aaCGTAAGACGGCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCACCGTGGGGATGGTAAAATGAATAGTAACGCTTATACCAATGATcc  >  2:813557/1‑238 (MQ=255)

                                                                                                                                                                                               gCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATaaa                                                                                                                                                                              >  2:264606/1‑56 (MQ=255)

                                                                                                                                                                                               gCATA‑TTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATaaa                                                                                                                                                                              <  1:264606/56‑1 (MQ=255)

ACAGAAATGACAAAATCACTATACTTGGTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCATCGTGGGGATGGTAAAATGAATAGTAACGCTTATACCAATGATCA  >  NC_000913/3798456‑3798875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A