Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F236 I1 R1 155 93.2 2393300 97.8% 2340647 180.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,535,528 T→G Q114P (CAG→CCG)  envZ ← sensory histidine kinase in two‑component regulatory system with OmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,535,5280TG98.7% 274.8 / NA 77Q114P (CAG→CCG) envZsensory histidine kinase in two‑component regulatory system with OmpR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (35/41);  minor base C (0/1);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGA  >  NC_000913/3535295‑3535759
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       
cGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGcc                                                                                                                                                                                                                                    >  1:1139502/1‑239 (MQ=255)
       gagaTCGCCAAACGGCGGGTTCTGGATACGAAAAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGAt                                                                                                                                                                                                                             <  2:973597/239‑1 (MQ=255)
                caccggtcggtTCGGGATACGAATAACCAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGa                                                                                                                                                                                                                    <  2:943433/236‑1 (MQ=255)
                cAACGGTCGGTTCTGGATCCGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGa                                                                                                                                                                                                                    <  1:928512/239‑1 (MQ=255)
                     gtcggtTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCa                                                                                                                                                                                                               >  1:1184542/1‑239 (MQ=255)
                      tcggtTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCa                                                                                                                                                                                                               <  1:416868/238‑1 (MQ=255)
                            cgggAAACGAATAACCGGCCCCGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGtt                                                                                                                                                                                                        <  2:103196/237‑1 (MQ=255)
                            cTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGtt                                                                                                                                                                                                        <  1:431691/239‑1 (MQ=255)
                                 tACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGcc                                                                                                                                                                                                   <  1:836357/239‑1 (MQ=255)
                                 aacaaaTAACCAGCCCCGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTgcgc                                                                                                                                                                                                    <  2:1142525/234‑1 (MQ=255)
                                  aCGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGGCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGccc                                                                                                                                                                                                  >  1:53819/1‑239 (MQ=255)
                                       tAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTa                                                                                                                                                                                                                       <  1:1108384/213‑1 (MQ=255)
                                       tAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTa                                                                                                                                                                                                                       >  2:1108384/1‑213 (MQ=255)
                                         aaCAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGGTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCa                                                                                                                                                                                            >  1:113287/1‑238 (MQ=255)
                                               cACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCCCTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCt                                                                                                                                                                                       <  1:286039/237‑1 (MQ=255)
                                                         cTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGCCCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGc                                                                                                                                                                            <  1:150494/238‑1 (MQ=255)
                                                          tATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAg                                                                                                                                                                          <  1:812172/239‑1 (MQ=255)
                                                            tCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTg                                                                                                                                                                                                                               >  1:102595/1‑184 (MQ=255)
                                                            tCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTg                                                                                                                                                                                                                               <  2:102595/184‑1 (MQ=255)
                                                               caaaTAGCTAAAGCGCCAGCGTAAGGCGGAACAGCGGAGAGAAAT‑CGCCCTGATAAATTTCGGTCAGCGGCAGGCGTACCCAGATATGGGGCGACAGCCAGGTTTTCAGCCAGACGAAGGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCGGCGCCATCGGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGAAGTCCTGCCTCTTCGGCAGCCTCg                                                                                                                                                                     <  2:654988/237‑1 (MQ=255)
                                                                cAATAGTCTAAGCGCAAGCGTTGTGGGGAACAGCGGAG‑GAAATGCGCCTTGAGGAATTGCGGTCAGCGGCACGCGTCCCCGGAGTTTGGGCGACACCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGGGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCg                                                                                                                                                                     <  2:1104458/238‑1 (MQ=255)
                                                                 aaTGGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGTTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGtt                                                                                                                                                                   <  1:5505/239‑1 (MQ=255)
                                                                 aaTAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTa                                                                                                                                                                                                                       <  1:870469/187‑1 (MQ=255)
                                                                 aaTAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTa                                                                                                                                                                                                                       >  2:870469/1‑187 (MQ=255)
                                                                 aaTAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGtt                                                                                                                                                                   >  1:644096/1‑239 (MQ=255)
                                                                  aTAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCCGACCTGCCTCTTCGGCAGCCTCGTTg                                                                                                                                                                  >  2:891310/1‑239 (MQ=255)
                                                                    aGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGa                                                                                                                                                                <  2:644096/239‑1 (MQ=255)
                                                                                aGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCTGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAgagcgaga                                                                                                                                                     >  1:206274/1‑238 (MQ=255)
                                                                                 gCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGGAGAGAGAGAt                                                                                                                                                    >  1:126271/1‑238 (MQ=255)
                                                                                           gAACAGCGGAAGAAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGTTATTGGGCGACAGCCCGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCa                                                                                                                                          <  2:892135/238‑1 (MQ=255)
                                                                                            aaCAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCct                                                                                                                                                                                   >  2:437111/1‑196 (MQ=255)
                                                                                            aaCAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCct                                                                                                                                                                                   <  1:437111/196‑1 (MQ=255)
                                                                                            aaCAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACg                                                                                                                                        >  1:755386/1‑239 (MQ=255)
                                                                                             agggCGGAGAAAAAT‑CGCCCTAGTGAATTGCGGTCAGCGGCAGTGGTACCCAGTTATTGGGCGACAGCCAGGTTTTCAGCCAGACGAAGGGCGACCTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGCCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACg                                                                                                                                        <  2:556098/235‑1 (MQ=255)
                                                                                             aCAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTc                                                                                                                                           >  1:1156238/1‑235 (MQ=255)
                                                                                                 cGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGa                                                                                                                                   >  1:1045800/1‑239 (MQ=255)
                                                                                                 cGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGa                                                                                                                                   >  1:1081589/1‑239 (MQ=255)
                                                                                                   gagagaAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGa                                                                                                                                   >  1:892135/1‑237 (MQ=255)
                                                                                                      agaAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGAtctc                                                                                                                               >  1:422844/1‑238 (MQ=255)
                                                                                                      agaAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGAtctc                                                                                                                               >  1:765831/1‑238 (MQ=255)
                                                                                                           t‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGa                                                                                                                                                                                                                              <  2:77688/138‑1 (MQ=255)
                                                                                                           t‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGa                                                                                                                                                                                                                              >  1:77688/1‑138 (MQ=255)
                                                                                                              gCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGa                                                                                                                       >  1:204543/1‑239 (MQ=255)
                                                                                                                cttgatcaATGTGGGTAAGCGGCACACGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGCGGAACTTTTGTTGACCCGAACGCGCACTTCCGTCGGGCCGCCCAGTTGGGGCGCCATCGGATGGGTTAAGAATTCATAGTGTTGCGCCCAACGCGGCCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCg                                                                                                                            <  2:953375/230‑1 (MQ=255)
                                                                                                                          ttCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc                                                                                                            >  2:293681/1‑238 (MQ=255)
                                                                                                                              gTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCgg                                                                                                                                                                             <  1:227739/169‑1 (MQ=255)
                                                                                                                              gTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCgg                                                                                                                                                                             >  2:227739/1‑169 (MQ=255)
                                                                                                                              gTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGCCGGAAAGCGGGAGGCAcaa                                                                                                        >  2:631587/1‑238 (MQ=255)
                                                                                                                                    ggCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGCCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACt                                                                                                  <  2:204543/238‑1 (MQ=255)
                                                                                                                                           gtccccAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCg                                                                                          <  2:113287/236‑1 (MQ=255)
                                                                                                                                            tccccAGATATTGGGCGACCGCCAGGTTTTCAGCCAGCCGACAGGCGAACTTTTGTTGACCTCAACGCGCCCTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTg                                                                                             <  1:778737/233‑1 (MQ=255)
                                                                                                                                                   atatTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcct                                                                                      >  2:428259/1‑235 (MQ=255)
                                                                                                                                                       tGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCCCTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGc                                                                                                                                                                            <  2:913551/145‑1 (MQ=255)
                                                                                                                                                       tGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGc                                                                                                                                                                            >  1:913551/1‑145 (MQ=255)
                                                                                                                                                         ggCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGtt                                                                                >  2:866222/1‑235 (MQ=255)
                                                                                                                                                           cGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGCCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGCGGCACAACCAACAGCGTGCCGTCCTCCAGTTGCAGtt                                                                          >  2:970601/1‑239 (MQ=255)
                                                                                                                                                           cGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCCTCGGCAGCCTCGTTGGAGTTGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGtt                                                                          >  1:90625/1‑239 (MQ=255)
                                                                                                                                                                 ccAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCACGTTGCAGTTTg                                                                        >  1:1067274/1‑235 (MQ=255)
                                                                                                                                                                         tCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAAAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAAc                                                            <  1:374555/239‑1 (MQ=255)
                                                                                                                                                                              gaagcgccAGGCACACTTTGTTTGCCCCCACCGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGTTTAAGAATTCATGGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCGCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACg                                                       <  2:755386/232‑1 (MQ=255)
                                                                                                                                                                                     cAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCg                                                <  2:422844/239‑1 (MQ=255)
                                                                                                                                                                                               tttGTTGACCTCAACGCGCACTCCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAGCGCAGCCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGa                                      <  2:1139502/239‑1 (MQ=255)
                                                                                                                                                                                               tttGTGGACCACACCGCGCCTTTCGGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGa                                      <  2:53819/239‑1 (MQ=255)
                                                                                                                                                                                               tttGTCGACCTCAGCGCGCACTTCCGTCGGCCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGa                                      <  2:76428/239‑1 (MQ=255)
                                                                                                                                                                                                  gttgACCGCAACGCGCACGTCCGGCGGGCCGCCCGGTGGCGGCGCCATCTGAGGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGCCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACtt                                   <  2:1045800/239‑1 (MQ=255)
                                                                                                                                                                                                     gACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTttat                                <  2:1081589/239‑1 (MQ=255)
                                                                                                                                                                                                           aaCGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGaaa                                                                                                                     >  2:712154/1‑148 (MQ=255)
                                                                                                                                                                                                           aaCGCGCACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGaaa                                                                                                                     <  1:712154/148‑1 (MQ=255)
                                                                                                                                                                                                                gcACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcatc                                                               >  1:428473/1‑197 (MQ=255)
                                                                                                                                                                                                                gcACTTCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcatc                                                               <  2:428473/197‑1 (MQ=255)
                                                                                                                                                                                                                     tCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACg                                                       <  2:698859/200‑1 (MQ=255)
                                                                                                                                                                                                                     tCCGTCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACg                                                       >  1:698859/1‑200 (MQ=255)
                                                                                                                                                                                                                         tCGGGCCGCCCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGc            >  1:480719/1‑239 (MQ=255)
                                                                                                                                                                                                                              ccgccCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcatc                                                               >  2:1067162/1‑183 (MQ=255)
                                                                                                                                                                                                                              ccgccCAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcatc                                                               <  1:1067162/183‑1 (MQ=255)
                                                                                                                                                                                                                                gccCGGTTGCCGCGCCATCTGTTGGCTTAAGATTTCATAGTGTTGCGCCCAGCGCAGCCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATCCGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATcg     <  2:126271/239‑1 (MQ=255)
                                                                                                                                                                                                                                  ccAGTTGCGGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATcgcg   <  1:866222/239‑1 (MQ=255)
                                                                                                                                                                                                                                       tgcggcGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGa  <  2:480719/235‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       
CGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAAT‑CGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGA  >  NC_000913/3535295‑3535759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A