breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 6,809 | T→G | K384T (AAA→ACA) | yaaJ ← | putative transporter |
RA | 47,341 | T→G | I32M (ATT→ATG) | kefF → | potassium‑efflux system ancillary protein for KefC, glutathione‑regulated; quinone oxidoreductase, FMN‑dependent |
RA | 58,850 | T→G | pseudogene (377/579 nt) | yabP → | pseudogene, pentapeptide repeats‑containing |
RA | 73,145 | T→G | K459N (AAA→AAC) | thiP ← | thiamine/thiamine pyrophosphate ABC transporter permease |
RA | 84,958 | T→G | I197M (ATT→ATG) | leuO → | global transcription factor |
RA | 95,418 | T→G | S257A (TCG→GCG) | murF → | UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase |
RA | 104,147 | T→G | S56A (TCC→GCC) | ftsA → | ATP‑binding cell division FtsK recruitment protein |
RA | 109,887 | A→C | I537L (ATT→CTT) | secA → | preprotein translocase subunit, ATPase |
JC JC | 111,051 | IS1 (–) +9 bp | coding (8‑16/390 nt) | mutT → | dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase |
RA | 113,356 | A→C | intergenic (‑137/‑88) | coaE ← / → guaC | dephospho‑CoA kinase/GMP reductase |
RA | 138,704 | A→C | intergenic (+71/+131) | cueO → / ← gcd | multicopper oxidase (laccase)/glucose dehydrogenase |
RA | 151,435 | A→C | L55L (CTT→CTG) | yadK ← | putative fimbrial‑like adhesin protein |
RA | 162,349 | A→C | Y82S (TAC→TCC) | hrpB → | putative ATP‑dependent helicase |
RA | 180,109 | T→G | L291L (CTT→CTG) | dgt → | deoxyguanosine triphosphate triphosphohydrolase |
RA | 242,498 | A→C | L269R (CTG→CGG) | fadE ← | acyl coenzyme A dehydrogenase |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 295,811 | T→G | E429A (GAA→GCA) | intF ← | CP4‑6 prophage; putative phage integrase |
RA | 343,447 | T→G | H188Q (CAT→CAG) | yahK → | broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing |
RA | 515,620 | T→G | M52L (ATG→CTG) | qmcA ← | PHB domain membrane‑anchored putative protease |
RA | 556,667 | A→C | V69V (GTT→GTG) | ybcJ ← | ribosome‑associated protein; putative RNA‑binding protein |
RA | 605,633 | A→C | V85G (GTG→GGG) | ybdF ← | DUF419 family protein |
RA | 607,856 | T→G | L7L (CTT→CTG) | hokE → | toxic polypeptide, small |
RA | 619,006 | A→C | G251G (GGA→GGC) | fepE → | regulator of length of O‑antigen component of lipopolysaccharide chains |
RA | 624,319 | A→C | D64E (GAT→GAG) | fepB ← | ferrienterobactin ABC transporter periplasmic binding protein |
RA | 682,878 | A→C | D172E (GAT→GAG) | hscC ← | Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor |
RA | 691,280 | T→G | I169L (ATT→CTT) | ybeX ← | putative ion transport |
RA | 699,371 | T→G | intergenic (‑194/+203) | asnB ← / ← umpH | asparagine synthetase B/UMP phosphatase |
RA | 743,458 | A→C | Y211S (TAT→TCT) | ybgI → | NIF3 family metal‑binding protein |
RA | 780,168 | T→G | Y191D (TAC→GAC) | ybgF → | periplasmic TolA‑binding protein |
RA | 825,545 | T→G | V188V (GTA→GTC) | ybhR ← | putative ABC transporter permease |
RA | 906,631 | T→G | E41D (GAA→GAC) | ybjS ← | putative NAD(P)H‑dependent oxidoreductase |
RA | 930,957 | A→C | N2K (AAT→AAG) | cydD ← | glutathione/cysteine ABC transporter export permease/ATPase |
RA | 946,920 | T→G | D350E (GAT→GAG) | ycaD → | putative MFS transporter, inner membrane protein |
RA | 960,657 | C→T | L132L (CTG→TTG) | ycaL → | putative peptidase‑related chaperone |
RA | 1,009,417 | A→C | D525A (GAT→GCT) | rlmL → | 23S rRNA m(2)G2445 and m(7)G2069 methyltransferases, SAM‑dependent |
RA | 1,078,882 | T→G | M1M (ATG→CTG) † | putA ← | fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase |
RA | 1,086,572 | T→G | K425N (AAA→AAC) | pgaC ← | biofilm PGA synthase PgaCD, catalytic subunit; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase |
RA | 1,139,916 | T→G | D513E (GAT→GAG) | flgK → | flagellar hook‑filament junction protein 1 |
RA | 1,176,289 | T→G | L288R (CTG→CGG) | lolC → | lipoprotein‑releasing system transmembrane protein |
RA | 1,183,048 | T→G | L191F (TTA→TTC) | potC ← | spermidine/putrescine ABC transporter permease |
RA | 1,192,441 | A→C | V64G (GTC→GGC) | hflD ← | putative lysogenization regulator |
RA | 1,197,360 | A→C | I58S (ATC→AGC) | ymfD ← | e14 prophage; putative SAM‑dependent methyltransferase |
RA | 1,297,609 | A→C | D171E (GAT→GAG) | adhE ← | fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase |
MC JC | 1,299,499 | Δ1,199 bp | intergenic (+254/‑485) | ychE → / → oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
RA | 1,311,079 | T→G | intergenic (‑214/‑10) | yciI ← / → tonB | putative DGPF domain‑containing enzyme/membrane spanning protein in TonB‑ExbB‑ExbD transport complex |
RA | 1,391,771 | A→C | F28C (TTT→TGT) | ycjY ← | S9 homolog non‑peptidase family protein |
RA | 1,402,453 | T→G | Q268P (CAG→CCG) | abgB ← | p‑aminobenzoyl‑glutamate hydrolase, B subunit |
RA | 1,417,487 | T→G | intergenic (‑101/+1) | recE ← / ← racC | Rac prophage; exonuclease VIII, 5' to 3' specific dsDNA exonuclease/Rac prophage; uncharacterized protein |
RA | 1,588,280 | T→G | K6T (AAG→ACG) | ydeR ← | putative fimbrial‑like adhesin protein |
RA | 1,632,251 | A→C | pseudogene (35/1284 nt) | ydfJ ← | pseudogene, MFS transporter family; interrupted by Qin prophage;Phage or Prophage Related; putative transport protein |
RA | 1,671,310 | A→C | intergenic (+358/‑66) | ynfM → / → asr | putative arabinose efflux transporter/acid shock‑inducible periplasmic protein |
RA | 1,688,772 | A→C | K66T (AAA→ACA) | manA → | mannose‑6‑phosphate isomerase |
RA | 1,714,643 | T→G | I89M (ATT→ATG) | gstA → | glutathionine S‑transferase |
RA | 1,756,688 | A→C | I331L (ATC→CTC) | pykF → | pyruvate kinase I |
RA | 1,768,120 | A→C | I189S (ATT→AGT) | ydiJ ← | putative FAD‑linked oxidoreductase |
RA | 1,815,451 | T→G | F529V (TTT→GTT) | katE → | catalase HPII, heme d‑containing |
RA | 1,822,043 | T→G | I72L (ATC→CTC) | osmE ← | osmotically‑inducible lipoprotein |
RA | 1,832,553 | T→G | F42L (TTT→TTG) | xthA → | exonuclease III |
RA | 1,905,833 | T→G | L49R (CTG→CGG) | mntP → | putative Mn(2+) efflux pump, mntR‑regulated |
RA | 1,907,569 | T→G | E8A (GAA→GCA) | yobF ← | DUF2527 family heat‑induced protein |
RA | 1,920,539 | T→G | L106W (TTG→TGG) | rsmF → | 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
RA | 1,937,568 | A→C | intergenic (+47/‑81) | yebK → / → pykA | putative DNA‑binding transcriptional regulator/pyruvate kinase II |
RA | 1,973,627 | T→G | K566N (AAA→AAC) | cheA ← | fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
JC JC | 1,979,486 | IS5 (+) +4 bp | intergenic (‑271/‑264) | insA ← / → uspC | IS1 repressor TnpA/universal stress protein |
RA | 2,008,526 | A→C | I84L (ATC→CTC) | yedE → | UPF0394 family sulphur transport domain‑containing inner membrane protein |
RA | 2,049,948 | T→G | S1671A (TCT→GCT) | yeeJ → | putative adhesin |
RA | 2,168,327 | A→C | intergenic (‑21/‑385) | yegR ← / → yegS | uncharacterized protein/phosphatidylglycerol kinase, metal‑dependent |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,226,626 | A→C | H215Q (CAT→CAG) | yohF ← | putative oxidoreductase |
RA | 2,278,251 | A→C | intergenic (‑14/+319) | yejG ← / ← bcr | uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter |
RA | 2,290,390 | A→C | intergenic (‑210/‑110) | yejO ← / → narP | pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system/response regulator in two‑component regulatory system with NarQ |
RA | 2,298,335 | T→G | V128V (GTA→GTC) | napB ← | nitrate reductase, small, cytochrome C550 subunit, periplasmic |
RA | 2,311,986 | A→C | L255R (CTG→CGG) | ompC ← | outer membrane porin protein C |
RA | 2,318,639 | A→C | F413C (TTT→TGT) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
RA | 2,323,004 | T→G | I380S (ATC→AGC) | atoC → | fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein |
RA | 2,375,801 | T→G | E54D (GAA→GAC) | menC ← | O‑succinylbenzoyl‑CoA synthase |
RA | 2,387,305 | G→A | P124P (CCG→CCA) | yfbM → | DUF1877 family protein |
RA | 2,404,261 | A→C | L118R (CTG→CGG) | nuoB ← | NADH:ubiquinone oxidoreductase, chain B |
RA | 2,408,074 | A→C | I172L (ATC→CTC) | alaA → | glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase |
RA | 2,414,947 | T→G | A67A (GCT→GCG) | pta → | phosphate acetyltransferase |
RA | 2,419,184 | A→C | intergenic (‑8/+50) | yfcD ← / ← yfcE | putative NUDIX hydrolase/phosphodiesterase activity on bis‑pNPP |
RA | 2,430,317 | A→C | F149L (TTT→TTG) | cvpA ← | colicin V production protein |
RA | 2,492,088 | T→G | L389F (TTA→TTC) | frc ← | formyl‑CoA transferase, NAD(P)‑binding |
RA | 2,523,162 | A→C | L275* (TTA→TGA) | xapB ← | xanthosine transporter |
RA | 2,656,000 | T→G | E120A (GAA→GCA) | pepB ← | aminopeptidase B |
RA | 2,667,681 | T→G | S56R (AGT→CGT) | hcaT ← | putative 3‑phenylpropionic transporter |
RA | 2,667,825 | A→C | W8G (TGG→GGG) | hcaT ← | putative 3‑phenylpropionic transporter |
RA | 2,706,202 | T→G | E308A (GAA→GCA) | lepA ← | back‑translocating elongation factor EF4, GTPase |
RA | 2,737,414 | A→C | K87N (AAA→AAC) | raiA → | cold shock protein associated with 30S ribosomal subunit |
RA | 2,778,463 | T→G | K1422Q (AAA→CAA) | ypjA ← | adhesin‑like autotransporter |
RA | 2,874,651 | T→G | N257H (AAT→CAT) | cysN ← | sulfate adenylyltransferase, subunit 1 |
RA | 2,876,976 | T→G | N132K (AAT→AAG) | iap → | aminopeptidase in alkaline phosphatase isozyme conversion |
RA | 2,899,375 | A→C | I15S (ATT→AGT) | ygcU ← | putative FAD‑linked oxidoreductase |
RA | 2,904,306 | T→G | intergenic (+932/+441) | ygcE → / ← queE | putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis |
RA | 2,982,326 | T→G | intergenic (‑144/+171) | araE ← / ← kduD | arabinose transporter/2‑dehydro‑3‑deoxy‑D‑gluconate 5‑dehydrogenase; KDG oxidoreductase; 20‑ketosteroid reductase |
RA | 3,009,624 | A→C | K287N (AAA→AAC) | ygeY → | putative peptidase |
RA | 3,025,296 | T→G | S316A (TCA→GCA) | xanQ → | xanthine permease |
RA | 3,076,684 | A→C | F258C (TTC→TGC) | yggP ← | putative Zn‑binding dehydrogenase |
RA | 3,088,259 | A→C | intergenic (+399/‑25) | metK → / → galP | S‑adenosylmethionine synthetase/D‑galactose transporter |
RA | 3,113,280 | T→G | V66V (GTA→GTC) | yghG ← | secretin (GspDbeta) OM localization lipoprotein pilotin |
RA | 3,135,804 | T→G | I190L (ATT→CTT) | pitB ← | phosphate transporter |
RA | 3,157,644 | A→C | intergenic (+194/‑6) | dkgA → / → yqhG | 2,5‑diketo‑D‑gluconate reductase A/DUF3828 family putative periplasmic protein |
RA | 3,173,660 | A→C | L579L (CTT→CTG) | parE ← | DNA topoisomerase IV, subunit B |
RA | 3,175,124 | A→C | L91L (CTT→CTG) | parE ← | DNA topoisomerase IV, subunit B |
RA | 3,193,396 | A→C | E178A (GAA→GCA) | yqiK → | PHB family membrane protein, function unknown |
RA | 3,198,644 | A→C | F333C (TTC→TGC) | glnE ← | fused deadenylyltransferase/adenylyltransferase for glutamine synthetase |
RA | 3,218,426 | A→C | H217Q (CAT→CAG) | aer ← | fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component |
RA | 3,328,817 | T→G | intergenic (‑102/‑146) | greA ← / → dacB | transcript cleavage factor/D‑alanyl‑D‑alanine carboxypeptidase |
RA | 3,334,302 | A→C | D198A (GAT→GCT) | ispB → | octaprenyl diphosphate synthase |
RA | 3,336,051 | A→C | D148E (GAT→GAG) | murA ← | UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
RA | 3,341,805 | A→C | K180N (AAA→AAC) | kdsD → | D‑arabinose 5‑phosphate isomerase |
RA | 3,349,202 | T→G | N203T (AAT→ACT) | mtgA ← | biosynthetic peptidoglycan transglycosylase |
RA | 3,399,779 | A→C | L67R (CTG→CGG) | mreC ← | cell wall structural complex MreBCD transmembrane component MreC |
RA | 3,468,138 | T→G | G572G (GGA→GGC) | chiA ← | periplasmic endochitinase |
RA | 3,473,612 | A→C | L157* (TTA→TGA) | rpsG ← | 30S ribosomal subunit protein S7 |
RA | 3,492,131 | A→C | F56C (TTT→TGT) | ppiA ← | peptidyl‑prolyl cis‑trans isomerase A (rotamase A) |
RA | 3,499,258 | A→C | intergenic (+57/‑190) | cysG → / → yhfL | fused siroheme synthase 1,3‑dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase/small lipoprotein |
RA | 3,535,528 | T→G | Q114P (CAG→CCG) | envZ ← | sensory histidine kinase in two‑component regulatory system with OmpR |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 3,571,007 | T→G | K105Q (AAA→CAA) | glgX ← | glycogen debranching enzyme |
RA | 3,616,561 | T→G | V127G (GTT→GGT) | nikC → | nickel ABC transporter permease |
RA | 3,618,985 | A→C | D133A (GAT→GCT) | nikR → | transcriptional repressor, Ni‑binding |
RA | 3,629,388 | T→G | K51Q (AAG→CAG) | rbbA ← | ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein |
RA | 3,632,997 | T→G | pseudogene (269/414 nt) | yhiL ← | pseudogene, DUF4049 family protein |
RA | 3,640,752 | T→G | intergenic (+207/‑110) | uspA → / → dtpB | universal stress global response regulator/dipeptide and tripeptide permease B |
RA | 3,708,873 | A→C | C535G (TGT→GGT) | eptB ← | KDO phosphoethanolamine transferase, Ca(2+)‑inducible |
RA | 3,725,176 | T→G | E48A (GAG→GCG) | glyQ ← | glycine tRNA synthetase, alpha subunit |
RA | 3,727,303 | A→C | L20R (CTC→CGC) | yiaA ← | YiaAB family inner membrane protein, tandem domains |
RA | 3,728,925 | T→G | V149V (GTA→GTC) | xylB ← | xylulokinase |
RA | 3,749,326 | A→C | D32A (GAT→GCT) | sgbU → | putative L‑xylulose 5‑phosphate 3‑epimerase |
RA | 3,752,058 | A→C | I23L (ATC→CTC) | yiaU → | putative DNA‑binding transcriptional regulator |
RA | 3,772,303 | A→C | K8T (AAA→ACA) | mtlA → | mannitol‑specific PTS enzyme: IIA, IIB and IIC components |
RA | 3,800,808 | T→G | D53A (GAT→GCT) | waaY ← | lipopolysaccharide core biosynthesis protein |
MC JC | 3,815,859 | Δ82 bp | [rph]–[rph] | [rph], [rph] | |
RA | 3,823,774 | A→C | N459H (AAC→CAC) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,829,992 | T→G | Y350D (TAT→GAT) | xanP → | xanthine permease |
RA | 3,923,819 | A→C | S605S (TCT→TCG) | mnmG ← | 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34 |
RA | 3,964,727 | A→C | F302V (TTT→GTT) | rhlB ← | ATP‑dependent RNA helicase |
RA | 4,003,071 | T→G | A41A (GCA→GCC) | yigG ← | PRK11371 family inner membrane protein |
RA | 4,046,716 | A→C | intergenic (‑114/‑250) | yihG ← / → polA | inner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease |
RA | 4,061,235 | A→C | Y24S (TAT→TCT) | yihM → | putative sugar phosphate isomerase |
RA | 4,118,936 | T→G | N132T (AAT→ACT) | rraA ← | ribonuclease E (RNase E) inhibitor protein |
RA | 4,158,027 | T→G | V393G (GTG→GGG) | argH → | argininosuccinate lyase |
RA | 4,186,603 | A→C | E418D (GAA→GAC) | rpoC → | RNA polymerase, beta prime subunit |
RA | 4,193,152 | A→C | L142R (CTC→CGC) | thiF ← | adenylyltransferase, modifies ThiS C‑terminus |
RA | 4,249,540 | T→G | intergenic (+229/‑14) | lamB → / → malM | maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein |
RA | 4,289,061 | A→C | D433A (GAT→GCT) | nrfA → | nitrite reductase, formate‑dependent, cytochrome |
RA | 4,291,339 | T→G | I288M (ATT→ATG) | nrfD → | formate‑dependent nitrite reductase, membrane subunit |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
RA | 4,514,053 | T→G | T86P (ACG→CCG) | fecB ← | ferric citrate ABC transporter periplasmic binding protein |
RA | 4,517,146 | T→G | N191T (AAT→ACT) | fecR ← | anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator |
RA | 4,552,330 | T→G | V232G (GTT→GGT) | uxuA → | mannonate hydrolase |
RA | 4,571,499 | A→C | L26V (TTG→GTG) | yjiR ← | putative DNA‑binding transcriptional regulator/putative aminotransferase |
RA | 4,572,080 | T→G | intergenic (+165/‑334) | yjiS → / → yjiT | DUF1127 family protein/pseudogene |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 3423877–3424233 | 3424463–3424242 | 10–587 | 18 [17] | [16] 18 | [rrlD] | [rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 1207790 = | 17 (0.190) | 82 (1.000) | 62/328 | 0.2 | 83.5% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 17 (0.210) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 17 (0.190) | 39 (0.480) | 32/328 | 1.1 | 70.6% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 17 (0.210) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related |