Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F40 I0 R1
|
13 |
14.1 |
329232 |
94.7% |
311782 |
135.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
USA300TCH1516_ALE |
2,532,298 |
A→T |
100% |
T15T (ACA→ACT) |
femA_3 → |
Aminoacyltransferase FemA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | USA300TCH1516_ALE | 2,532,298 | 0 | A | T | 100.0%
| 46.1
/ NA
| 19 | T15T (ACA→ACT) | femA_3 | Aminoacyltransferase FemA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (6/13); total (6/13) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACA > USA300TCH1516_ALE/2532167‑2532427
|
tttGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTActacaa < 2:121108/136‑1 (MQ=255)
aaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGTATGAGTTCAATTTTTTTACTATAAAGCATTTTTCACAATacac < 1:58116/136‑1 (MQ=255)
aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc < 2:61080/136‑1 (MQ=255)
attaATAAATTTAAGAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAc < 2:129981/136‑1 (MQ=255)
tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGc < 2:21052/136‑1 (MQ=255)
gagGGATAGGATAAAATACACTTTGTAACGTTGACTTCAGATGAGTTCAATGCGTTTACTACAAAGAATTTTTCACATTACACACAATCATCTATTCATTACAATCATATAGTTTATTTAAAAGGCGATGTGCATc < 2:44596/136‑1 (MQ=255)
gatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGTTATTCATTATTATTTTAGTGTTTATTTAAAAGGCGATGTGCATCTTGt < 1:106779/136‑1 (MQ=255)
tGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAAt > 1:25942/1‑136 (MQ=255)
gTAACGTTGACTTCGGATGAATTAAATACAAAAACTACAAAACATAATTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAAGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATg > 1:6423/1‑136 (MQ=255)
cGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa > 2:75663/1‑136 (MQ=255)
tttaCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGTTGTGCATTTTTTTGGGGTTAAAGATGACAATGGTCAAg < 1:143090/133‑1 (MQ=255)
tGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGt < 2:58567/136‑1 (MQ=255)
gACTTCGGATGAGTTCAAGGTGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTg < 2:156990/136‑1 (MQ=255)
cTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAATGCGATGTGTATCTTGTATGGGTTAAAGATGACAATGGTCAAGTGAt < 1:51429/136‑1 (MQ=255)
ttCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGTATCTTGTTGTGTTTAAAGATGACAATGGTCAAGTGAtt < 1:95087/136‑1 (MQ=255)
ttCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGTATCTTGTAGGGTTTAAAGATGACAATGGTCAAGTGAtt < 1:157932/136‑1 (MQ=255)
ttCGGATGAGTTCAATGCGTTTACTACAAAGCATATTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt > 1:149905/1‑136 (MQ=255)
ttCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCACTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAAGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGACGCt > 2:122997/1‑136 (MQ=255)
actGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTAGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAt > 1:7106/3‑136 (MQ=255)
gTTTATTACAAAGCATTTTTCAGATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACa < 2:141467/136‑1 (MQ=255)
|
TTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACA > USA300TCH1516_ALE/2532167‑2532427
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
