Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F59 I1 R1 29 14.4 307489 99.5% 305951 148.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 1,522,796 T→A K171I (AAA→ATA)  dnaD ← DNA replication protein DnaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,522,7960TA100.0% 17.7 / NA 6K171I (AAA→ATA) dnaDDNA replication protein DnaD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (2/4);  total (2/4)

ATCAAATTTAGGTACCGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTTTATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGTTTCTATTTCATATGGA  >  USA300TCH1516_ALE/1522654‑1522894
                                                                                                                                              |                                                                                                  
aTCAAATTTAGGTACCGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTc                                                                                               >  1:136985/1‑148 (MQ=255)
         aGGTACCGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTc                                                                                      <  1:273969/148‑1 (MQ=255)
               cGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCg                                                                                <  1:73894/148‑1 (MQ=255)
                               gTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGta                                                                >  1:263778/1‑148 (MQ=255)
                                                                             cAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGt                  <  1:200738/148‑1 (MQ=255)
                                                                                             gTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGTTTCTATTTCATATGga  <  1:267117/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                  
ATCAAATTTAGGTACCGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTTTATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGTTTCTATTTCATATGGA  >  USA300TCH1516_ALE/1522654‑1522894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A