Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F184 I1 R1 32 17.0 340811 99.5% 339106 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 2,241,539 T→C intergenic (‑182/+284) murA2 ← / ← fba UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase 2/Fructose‑bisphosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,241,5390TC100.0% 30.6 / NA 9intergenic (‑182/+284)murA2/fbaUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase 2/Fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/2);  total (7/2)

TTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCACAATCA  >  USA300TCH1516_ALE/2241420‑2241666
                                                                                                                       |                                                                                                                               
tttACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAata                                                                                                    <  1:73073/149‑1 (MQ=255)
               aaaTAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGa                                                                                     >  1:218571/1‑149 (MQ=255)
                                 gTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAAt                                                                   >  1:264725/1‑149 (MQ=255)
                                                ttCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACg                                                    >  1:132120/1‑149 (MQ=255)
                                                                               tCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCt                     <  1:170720/149‑1 (MQ=255)
                                                                                                tGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaat    >  1:23623/1‑149 (MQ=255)
                                                                                                 gCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaatc   >  1:303749/1‑149 (MQ=255)
                                                                                                  cTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaatca  >  1:303520/1‑149 (MQ=255)
                                                                                                  cTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaatca  >  1:37369/1‑149 (MQ=255)
                                                                                                                       |                                                                                                                               
TTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCACAATCA  >  USA300TCH1516_ALE/2241420‑2241666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A