Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F2 I1 R1
|
251 |
90.0 |
5200563 |
86.9% |
4519289 |
59.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
Exported |
2,761,561 |
C→T |
24.8% |
I125I (ATC→ATT) |
aroE → |
dehydroshikimate reductase, NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | Exported | 2,761,561 | 0 | C | T | 24.8%
| 159.3
/ 53.7
| 89 | I125I (ATC→ATT) | aroE | dehydroshikimate reductase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (43/23); new base T (2/20); total (46/43) |
| Fisher's exact test for biased strand distribution p-value = 3.85e-06 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 |
GCTTGTTAAGCGATCTGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCACTCCTTTCCCTGGACTGTGCGGTGACAATAAC > Exported/2761500‑2761629
|
gCTTGTTAAGCGATCTGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCtggt > 1:2553881/1‑71 (MQ=255)
ttGTTAAGCGATCTGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCtggtg > 1:4423823/1‑70 (MQ=255)
ttGTTAAGCGATCTGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCtggtg > 1:5007569/1‑70 (MQ=255)
ttGTTAAGCGATCTGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCtgg > 1:1923529/1‑68 (MQ=255)
gTTAAGCGATCTGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:3297770/1‑71 (MQ=255)
gTTAAGCGATCTGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:1113408/1‑71 (MQ=255)
gTTAAGCGATCTGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:633537/1‑71 (MQ=255)
aGCGATCTGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCAtc > 1:1922980/1‑71 (MQ=255)
aGCGATCTGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCAtc > 1:1512763/1‑71 (MQ=255)
aGCGATCTGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCAtc > 1:985915/1‑71 (MQ=255)
aGCGATCTGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCAt > 1:4827825/1‑70 (MQ=255)
tGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCAt < 1:4377200/63‑1 (MQ=255)
tGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCAt < 1:270298/63‑1 (MQ=255)
tGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCAt < 1:219187/63‑1 (MQ=255)
tGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCAt < 1:4114389/63‑1 (MQ=255)
aCGTCTGTCTTTTATCCGTCCTGGTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTCGCGGCGtac > 1:3357890/1‑71 (MQ=255)
aCGTCTGTCTTTTATCCGTCCTGGTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTCGCGGCGtac > 1:3362580/1‑71 (MQ=255)
cGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGtact < 1:4538555/71‑1 (MQ=255)
ctgtctTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCAtctc < 1:5040750/58‑1 (MQ=255)
ctgtctTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCAtctc < 1:1162690/58‑1 (MQ=255)
ctgtctTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCAtctc < 1:1235028/58‑1 (MQ=255)
ctgtctTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCAtctc < 1:3849935/58‑1 (MQ=255)
tctTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTcgcg < 1:820670/58‑1 (MQ=255)
tctTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCgcggcg < 1:674647/61‑1 (MQ=255)
tctTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCgcggcg < 1:102611/61‑1 (MQ=255)
tctTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTactact < 1:4671181/68‑1 (MQ=255)
tctTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTactact < 1:2993713/68‑1 (MQ=255)
tctTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGc < 1:5016678/70‑1 (MQ=255)
ttttATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCa > 1:2638045/1‑70 (MQ=255)
ttttATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCAc > 1:1744720/1‑71 (MQ=255)
ttATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCACTc > 1:5023199/1‑71 (MQ=255)
ccGCCCTGGTTTACGTATTCTGCTTATCGGCGCtggtgg < 1:3883988/39‑1 (MQ=255)
ccGCCCTGGTTTACGTATTCTGCTTATCGGCGCtggtgg < 1:1102065/39‑1 (MQ=255)
gCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:3612809/1‑39 (MQ=255)
gCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:5012942/1‑39 (MQ=255)
gCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:4227458/1‑39 (MQ=255)
gCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:3550653/1‑39 (MQ=255)
gCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:743701/1‑39 (MQ=255)
gCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:1858196/1‑39 (MQ=255)
gCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:2036268/1‑39 (MQ=255)
gCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:2075806/1‑39 (MQ=255)
gCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:1351304/1‑39 (MQ=255)
gCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:2237345/1‑39 (MQ=255)
gCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAg > 1:125031/1‑39 (MQ=255)
cccTGGTTTACGTATTCTGCTTATCGGCGCTTGTGGAGCATCTCGCGGCGTACTACTGCCACTCCTTTccc < 1:3054529/71‑1 (MQ=255)
cTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCACTCCTTTCCCTg < 1:734499/71‑1 (MQ=255)
tGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCgcggc < 1:3019179/46‑1 (MQ=255)
tGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCgcgg > 1:4434808/1‑45 (MQ=255)
tGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCgcgg > 1:2003497/1‑45 (MQ=255)
tGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCgcgg > 1:2159470/1‑45 (MQ=255)
tGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCgcgg > 1:3019036/1‑45 (MQ=255)
tGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCgcgg > 1:3766502/1‑45 (MQ=255)
ggTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCgcggc < 1:1241789/45‑1 (MQ=255)
gTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCACTCCTTTCCCTgg < 1:1421236/69‑1 (MQ=255)
gTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCACTCCTTTCCCTGGAc > 1:2919713/1‑71 (MQ=255)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:543032/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:543007/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:5102002/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:636358/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:509029/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:744446/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:1550104/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:877288/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:1976660/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:4190096/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:2704051/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:3909830/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:4143495/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:4197080/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:4301482/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:3305618/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:4393739/40‑1 (MQ=38)
gTTTACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:3233551/40‑1 (MQ=38)
tttACGTTTTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:3932891/39‑1 (MQ=37)
tttACGTATTCTGCTTATCGGCGCTGGTGGAGCAtc > 1:2509481/1‑36 (MQ=255)
tttACGTATTCTGCTCATTGGCGCTGGTGGAGCATCTcg < 1:1330471/39‑1 (MQ=38)
ttACGTATTCTGCTTATCGGCGCTGGTGGAGCAtct > 1:1843178/1‑36 (MQ=255)
ttACGTATTCTGCTTATCGGCGCTGGTGGAGCAtct > 1:11195/1‑36 (MQ=255)
ttACGTATTCTGCTTATCGGCGCTGGTGGAGCAtct > 1:960666/1‑36 (MQ=255)
ttACGTATTCTGCTTATCGGCGCTGGTGGAGCAtct > 1:383840/1‑36 (MQ=255)
ttACGTATTCTGCTTATCGGCGCTGGTGGAGCAtct > 1:3508146/1‑36 (MQ=255)
tACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGtac > 1:4801474/1‑45 (MQ=255)
gTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCACTCCTTTCCCTGGACtgtg < 1:1316618/69‑1 (MQ=255)
tATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCACTCCTTTCCCTGGACTGTGCgg > 1:1027640/1‑71 (MQ=255)
aTTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCACTCCTTTCCCTGGACTGTGCGGt > 1:1461702/1‑71 (MQ=255)
aTTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCACTCCTTCCCCTGGACTGTGCGGt > 1:4758256/1‑71 (MQ=255)
ttCTGCTTATCGGCGCTGGTTGAGCATCTCGCGGCGTACTACTGCCACTCCTTTCCCTGGACTGTGCGGt > 1:4611303/1‑70 (MQ=255)
cTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCACTCCTTTCCCTGGACTGTGCGGTGa > 1:3337637/1‑70 (MQ=255)
cTGCTTATAGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCACTCCTTTCCCTGGACTGTGCGGTGa > 1:4916583/1‑70 (MQ=255)
cGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCACTCCTTTCCCTGGACTCTGCGGTGACAATAAc > 1:3671741/1‑69 (MQ=255)
|
GCTTGTTAAGCGATCTGGAACGTCTGTCTTTTATCCGCCCTGGTTTACGTATTCTGCTTATCGGCGCTGGTGGAGCATCTCGCGGCGTACTACTGCCACTCCTTTCCCTGGACTGTGCGGTGACAATAAC > Exported/2761500‑2761629
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
