breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 28,911 T→C 6.5% I139I (ATT→ATC carB → carbamoyl‑phosphate synthase large subunit
RA 28,923 C→T 6.8% T143T (ACC→ACT carB → carbamoyl‑phosphate synthase large subunit
RA 30,369 T→C 7.0% D625D (GAT→GAC carB → carbamoyl‑phosphate synthase large subunit
RA 42,526 C→T 15.9% G223G (GGC→GGT fixC → predicted oxidoreductase, FAD/NAD(P)‑binding domain
RA 61,959 A→G 12.5% Y500Y (TAT→TAC polB ← DNA polymerase II
RA 61,962 A→G 12.3% F499F (TTT→TTC polB ← DNA polymerase II
RA 61,968 A→G 10.6% N497N (AAT→AAC polB ← DNA polymerase II
RA 61,980 T→C 12.3% K493K (AAA→AAG polB ← DNA polymerase II
RA 64,795 G→T 8.4% G270G (GGC→GGA leuC ← 3‑isopropylmalate isomerase subunit, dehydratase component
RA 86,476 T→A 27.6% T324T (ACT→ACA murC → UDP‑N‑acetylmuramate:L‑alanine ligase
RA 86,479 T→G 25.0% G325G (GGT→GGG murC → UDP‑N‑acetylmuramate:L‑alanine ligase
RA 108,281 C→G 10.7% L175L (CTC→CTG aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding
RA 108,284 T→C 10.7% S176S (TCT→TCC aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding
RA 108,287 C→G 9.8% S177S (TCC→TCG aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding
RA 108,290 T→C 10.0% Y178Y (TAT→TAC aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding
RA 108,314 A→C 6.8% E186D (GAA→GAC aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding
RA 109,695 C→T 14.7% H647Y (CAC→TAC)  aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding
RA 123,547 C→T 12.5% intergenic (+174/+28) cueO → / ← gcd multicopper oxidase (laccase)/glucose dehydrogenase
RA 123,550 A→G 12.6% intergenic (+177/+25) cueO → / ← gcd multicopper oxidase (laccase)/glucose dehydrogenase
RA 143,709 T→C 12.5% Y53C (TAC→TGC)  pcnB ← poly(A) polymerase I
RA 168,689 G→T 13.7% intergenic (‑358/‑10) map ← / → rpsB methionine aminopeptidase/30S ribosomal subunit protein S2
RA 168,773 G→A 19.2% P25P (CCG→CCA rpsB → 30S ribosomal subunit protein S2
RA 168,776 A→G 16.5% K26K (AAA→AAG rpsB → 30S ribosomal subunit protein S2
RA 168,780 A→G 16.8% K28E (AAG→GAG) ‡ rpsB → 30S ribosomal subunit protein S2
RA 168,781 A→C 16.9% K28T (AAG→ACG) ‡ rpsB → 30S ribosomal subunit protein S2
RA 187,851 G→A 6.0% A136T (GCG→ACG)  accA → acetylCoA carboxylase, carboxytransferase, alpha subunit
RA 204,511 C→T 100% intergenic (+111/‑73) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 204,556 A→G 100% intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 214,925 A→C 89.2% D12A (GAT→GCT)  dnaQ → DNA polymerase III epsilon subunit
RA 214,931 A→C 89.1% E14A (GAA→GCA)  dnaQ → DNA polymerase III epsilon subunit
RA 217,959 A→G 23.9% intergenic (‑51/‑55) yafU ← / → yafF predicted inner membrane protein/conserved hypothetical protein
RA 217,968 C→T 24.4% intergenic (‑60/‑46) yafU ← / → yafF predicted inner membrane protein/conserved hypothetical protein
RA 217,996 A→T 18.5% intergenic (‑88/‑18) yafU ← / → yafF predicted inner membrane protein/conserved hypothetical protein
RA 275,767 C→G 13.5% T136T (ACG→ACC cyoE ← protoheme IX farnesyltransferase
RA 275,782 G→A 13.9% H131H (CAC→CAT cyoE ← protoheme IX farnesyltransferase
RA 275,785 G→A 13.5% R130R (CGC→CGT cyoE ← protoheme IX farnesyltransferase
RA 373,682 G→T 6.3% A178S (GCG→TCG)  fepE → regulator of length of O‑antigen component of lipopolysaccharide chains
RA 385,795 T→C 6.7% C335C (TGT→TGC cstA → carbon starvation protein
RA 385,801 G→C 8.0% A337A (GCG→GCC cstA → carbon starvation protein
RA 385,804 G→C 7.9% V338V (GTG→GTC cstA → carbon starvation protein
RA 385,807 T→G 6.8% S339S (TCT→TCG cstA → carbon starvation protein
RA 385,816 T→C 7.0% H342H (CAT→CAC cstA → carbon starvation protein
RA 484,413 C→T 12.4% R73R (CGC→CGT tolB → periplasmic protein
RA 484,420 C→T 12.9% L76L (CTG→TTG)  tolB → periplasmic protein
RA 499,328 C→T 13.9% V306I (GTT→ATT)  modF ← fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily
RA 518,462 T→C 8.1% A162A (GCT→GCC bioC → predicted methltransferase, enzyme of biotin synthesis
RA 550,934 T→C 9.1% K4K (AAA→AAG ybiV ← predicted hydrolase
RA 572,219 A→G 13.4% I176I (ATT→ATC aqpZ ← aquaporin
RA 710,033 G→C 18.7% intergenic (‑159/+102) yceJ ← / ← yceO predicted cytochrome b561/hypothetical protein
RA 710,036 G→C 17.3% intergenic (‑162/+99) yceJ ← / ← yceO predicted cytochrome b561/hypothetical protein
RA 710,041 G→A 15.5% intergenic (‑167/+94) yceJ ← / ← yceO predicted cytochrome b561/hypothetical protein
RA 719,427 C→T 6.4% A254V (GCC→GTC)  mviN → predicted inner membrane protein
RA 773,925 C→T 9.0% L94L (TTG→TTA ymfC ← 23S rRNA pseudouridine synthase
RA 775,580 A→G 8.8% K401K (AAA→AAG icd → isocitrate dehydrogenase, specific for NADP+
RA 775,607 C→A 9.4% D410E (GAC→GAA icd → isocitrate dehydrogenase, specific for NADP+
RA 809,202 T→C 5.0% K275K (AAA→AAG ychF ← predicted GTP‑binding protein
RA 826,746 T→A 11.0% S381S (TCT→TCA ychP → predicted invasin
RA 949,284 T→A 5.1% Y518F (TAT→TTT)  dos ← cAMP phosphodiesterase, heme‑regulated
RA 1,036,505 G→A 100% A632A (GCG→GCA rsxC → fused predicted 4Fe‑4S ferredoxin‑type protein
RA 1,080,862 A→G 11.9% N220N (AAT→AAC ydhV ← predicted oxidoreductase
RA 1,185,005 G→A 13.4% V324I (GTT→ATT) ‡ yeaG → conserved hypothetical protein with nucleoside triphosphate hydrolase domain
RA 1,185,007 T→C 13.3% V324V (GTT→GTC) ‡ yeaG → conserved hypothetical protein with nucleoside triphosphate hydrolase domain
RA 1,185,010 C→T 10.6% Y325Y (TAC→TAT yeaG → conserved hypothetical protein with nucleoside triphosphate hydrolase domain
RA 1,185,032 T→C 9.8% L333L (TTG→CTG)  yeaG → conserved hypothetical protein with nucleoside triphosphate hydrolase domain
RA 1,185,038 A→G 8.4% I335V (ATT→GTT) ‡ yeaG → conserved hypothetical protein with nucleoside triphosphate hydrolase domain
RA 1,185,040 T→G 8.4% I335M (ATT→ATG) ‡ yeaG → conserved hypothetical protein with nucleoside triphosphate hydrolase domain
RA 1,191,074 A→C 9.6% intergenic (+415/+485) yeaJ → / ← rnd predicted diguanylate cyclase/ribonuclease D
RA 1,223,810 T→C 11.1% S795S (AGT→AGC yebT → conserved hypothetical protein
RA 1,305,362 T→C 15.1% T233A (ACG→GCG)  yeeE ← predicted inner membrane protein
RA 1,305,959 A→G 6.7% Y34H (TAT→CAT)  yeeE ← predicted inner membrane protein
RA 1,305,960 C→T 6.4% M33I (ATG→ATA yeeE ← predicted inner membrane protein
RA 1,305,967 C→T 6.4% R31H (CGC→CAC)  yeeE ← predicted inner membrane protein
RA 1,314,255 T→C 9.1% H170H (CAT→CAC hisB → fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase
RA 1,315,169 C→T 13.7% N119N (AAC→AAT hisH → imidazole glycerol phosphate synthase, glutamine amidotransferase subunit
RA 1,319,252 G→A 12.2% L214L (CTG→TTG)  ugd ← UDP‑glucose 6‑dehydrogenase
RA 1,319,256 G→A 11.2% N212N (AAC→AAT ugd ← UDP‑glucose 6‑dehydrogenase
RA 1,319,262 G→A 10.1% Y210Y (TAC→TAT) ‡ ugd ← UDP‑glucose 6‑dehydrogenase
RA 1,319,263 T→A 9.7% Y210F (TAC→TTC) ‡ ugd ← UDP‑glucose 6‑dehydrogenase
RA 1,319,265 C→T 10.0% A209A (GCG→GCA ugd ← UDP‑glucose 6‑dehydrogenase
RA 1,319,268 C→A 9.9% V208V (GTG→GTT ugd ← UDP‑glucose 6‑dehydrogenase
RA 1,319,271 G→T 10.0% R207R (CGC→CGA ugd ← UDP‑glucose 6‑dehydrogenase
RA 1,319,277 C→A 9.3% A205A (GCG→GCT ugd ← UDP‑glucose 6‑dehydrogenase
RA 1,336,081 G→A 10.4% G280G (GGC→GGT gmd ← GDP‑D‑mannose dehydratase, NAD(P)‑binding
RA 1,336,113 G→A 10.4% L270L (CTG→TTG)  gmd ← GDP‑D‑mannose dehydratase, NAD(P)‑binding
RA 1,393,077 T→C 6.1% K241E (AAA→GAA)  fruK ← fructose‑1‑phosphate kinase
RA 1,403,006 G→A 11.1% P283P (CCG→CCA rtn → conserved hypothetical protein
RA 1,418,305 T→C 11.2% I85T (ATC→ACC)  narP → DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX
RA 1,488,632 A→G 8.5% L132L (CTT→CTC nuoM ← NADH:ubiquinone oxidoreductase, membrane subunit M
RA 1,488,647 A→C 9.5% V127V (GTT→GTG nuoM ← NADH:ubiquinone oxidoreductase, membrane subunit M
RA 1,488,650 G→A 9.3% G126G (GGC→GGT nuoM ← NADH:ubiquinone oxidoreductase, membrane subunit M
RA 1,488,719 T→G 10.0% L103L (CTA→CTC nuoM ← NADH:ubiquinone oxidoreductase, membrane subunit M
RA 1,498,221 T→C 13.8% K541K (AAA→AAG nuoC ← NADH:ubiquinone oxidoreductase, chain C,D
RA 1,502,847 A→T 11.3% intergenic (‑220/‑700) lrhA ← / → yfbQ DNA‑binding transcriptional repressor/predicted aminotransferase
RA 1,515,674 A→G 7.8% A114A (GCT→GCC cvpA ← membrane protein required for colicin V production
RA 1,614,452 C→T 7.2% intergenic (‑77/+140) eutH ← / ← eutG predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization
RA 1,614,453 G→C 7.2% intergenic (‑78/+139) eutH ← / ← eutG predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization
RA 1,614,454 T→C 7.3% intergenic (‑79/+138) eutH ← / ← eutG predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization
RA 1,614,455 A→T 8.8% intergenic (‑80/+137) eutH ← / ← eutG predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization
RA 1,614,459 T→C 10.6% intergenic (‑84/+133) eutH ← / ← eutG predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization
RA 1,614,466 G→A 16.7% intergenic (‑91/+126) eutH ← / ← eutG predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization
RA 1,614,480 C→T 24.2% intergenic (‑105/+112) eutH ← / ← eutG predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization
RA 1,614,492 T→C 29.6% intergenic (‑117/+100) eutH ← / ← eutG predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization
RA 1,635,083 T→C 13.4% D407D (GAT→GAC acrD → aminoglycoside/multidrug efflux system
RA 1,635,086 C→T 12.4% D408D (GAC→GAT acrD → aminoglycoside/multidrug efflux system
RA 1,635,104 A→G 10.5% E414E (GAA→GAG acrD → aminoglycoside/multidrug efflux system
RA 1,658,871 G→A 12.7% P357P (CCG→CCA ppx → exopolyphosphatase
RA 1,664,022 A→G 7.8% R400R (CGT→CGC guaA ← GMP synthetase
RA 1,664,028 C→G 6.9% L398L (CTG→CTC) ‡ guaA ← GMP synthetase
RA 1,664,030 G→C 6.4% L398V (CTG→GTG) ‡ guaA ← GMP synthetase
RA 1,664,040 C→G 7.1% P394P (CCG→CCC guaA ← GMP synthetase
RA 1,664,058 A→G 6.8% G388G (GGT→GGC guaA ← GMP synthetase
RA 1,664,061 A→G 7.4% I387I (ATT→ATC guaA ← GMP synthetase
RA 1,664,067 A→G 8.1% R385R (CGT→CGC guaA ← GMP synthetase
RA 1,664,079 T→C 10.3% K381K (AAA→AAG guaA ← GMP synthetase
RA 1,755,953 G→A 10.6% D181N (GAC→AAC)  pssA → phosphatidylserine synthase
RA 1,755,958 T→A 9.7% I182I (ATT→ATA pssA → phosphatidylserine synthase
RA 1,763,540 A→G 34.7% noncoding (304/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,763,556 T→C 33.8% noncoding (288/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,763,558 G→C 18.2% noncoding (286/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,763,559 G→T 18.1% noncoding (285/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,763,580 G→T 11.2% noncoding (264/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,763,636 A→G 14.6% noncoding (208/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,768,269 A→G 22.4% T143T (ACT→ACC rluD ← 23S rRNA pseudouridine synthase
RA 1,804,090 G→A 84.1% G52G (GGG→GGA mprA → DNA‑binding transcriptional regulator
RA 1,829,982 A→T 9.8% G186G (GGA→GGT pphB → serine/threonine‑specific protein phosphatase 2
RA 1,830,030 T→A 6.5% I202I (ATT→ATA pphB → serine/threonine‑specific protein phosphatase 2
RA 1,844,947 A→G 8.5% R46R (CGT→CGC cysN ← sulfate adenylyltransferase, subunit 1
RA 1,844,959 A→G 7.8% T42T (ACT→ACC cysN ← sulfate adenylyltransferase, subunit 1
RA 1,858,847 C→T 18.6% K245K (AAG→AAA cysI ← sulfite reductase, beta subunit, NAD(P)‑binding
RA 1,876,422 A→G 5.5% G334G (GGT→GGC eno ← enolase
RA 1,876,425 T→C 5.5% K333K (AAA→AAG eno ← enolase
RA 1,876,428 T→C 5.5% E332E (GAA→GAG) ‡ eno ← enolase
RA 1,876,430 C→G 5.7% E332Q (GAA→CAA) ‡ eno ← enolase
RA 1,876,434 A→G 6.4% G330G (GGT→GGC eno ← enolase
RA 1,876,440 T→C 6.4% K328K (AAA→AAG eno ← enolase
RA 1,876,461 T→G 6.0% V321V (GTA→GTC eno ← enolase
RA 1,884,163 G→A 12.2% A107V (GCA→GTA)  rumA ← 23S rRNA (uracil‑5)‑methyltransferase
RA 1,892,019:1 +GC 100% intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein
RA 2,007,627 T→A 5.5% S92C (AGC→TGC)  hybA ← hydrogenase 2 4Fe‑4S ferredoxin‑type component
RA 2,064,986 C→T 7.4% N409N (AAC→AAT rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 2,064,992 T→C 9.3% G411G (GGT→GGC rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 2,065,001 A→G 9.3% K414K (AAA→AAG rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 2,065,007 T→G 8.2% V416V (GTT→GTG rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 2,065,013 A→G 5.2% K418K (AAA→AAG rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 2,065,025 C→T 9.1% R422R (CGC→CGT rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 2,065,028 T→C 8.8% R423R (CGT→CGC rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 2,065,031 T→C 5.5% G424G (GGT→GGC rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 2,065,034 C→T 7.7% Y425Y (TAC→TAT rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 2,068,677 A→G 5.3% N371N (AAT→AAC aer ← fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component
RA 2,155,529 A→G 14.3% intergenic (+40/+146) dacB → / ← obgE D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
RA 2,155,567 A→T 7.3% intergenic (+78/+108) dacB → / ← obgE D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
RA 2,253,051 A→G 41.7% T1329T (ACT→ACC rpoC ← RNA polymerase, beta prime subunit
RA 2,253,057 C→T 37.9% E1327E (GAG→GAA rpoC ← RNA polymerase, beta prime subunit
RA 2,253,072 C→G 31.3% A1322A (GCG→GCC rpoC ← RNA polymerase, beta prime subunit
RA 2,253,075 G→C 31.3% S1321S (TCC→TCG rpoC ← RNA polymerase, beta prime subunit
RA 2,254,740 A→G 10.8% G766G (GGT→GGC rpoC ← RNA polymerase, beta prime subunit
RA 2,254,746 A→C 12.2% R764R (CGT→CGG rpoC ← RNA polymerase, beta prime subunit
RA 2,254,764 T→C 13.7% P758P (CCA→CCG rpoC ← RNA polymerase, beta prime subunit
RA 2,254,767 C→G 13.5% T757T (ACG→ACC rpoC ← RNA polymerase, beta prime subunit
RA 2,254,770 T→C 15.1% E756E (GAA→GAG rpoC ← RNA polymerase, beta prime subunit
RA 2,254,779 G→C 16.1% S753S (TCC→TCG rpoC ← RNA polymerase, beta prime subunit
RA 2,254,785 A→G 15.9% D751D (GAT→GAC rpoC ← RNA polymerase, beta prime subunit
RA 2,256,930 A→G 40.0% G36G (GGT→GGC rpoC ← RNA polymerase, beta prime subunit
RA 2,266,165 G→A 16.4% I93I (ATC→ATT tufB ← protein chain elongation factor EF‑Tu
RA 2,266,174 T→C 15.1% K90K (AAA→AAG tufB ← protein chain elongation factor EF‑Tu
RA 2,266,177 A→G 15.9% V89V (GTT→GTC) ‡ tufB ← protein chain elongation factor EF‑Tu
RA 2,266,179 C→T 15.9% V89I (GTT→ATT) ‡ tufB ← protein chain elongation factor EF‑Tu
RA 2,266,180 A→G 16.1% Y88Y (TAT→TAC tufB ← protein chain elongation factor EF‑Tu
RA 2,266,192 C→G 22.2% G84G (GGG→GGC tufB ← protein chain elongation factor EF‑Tu
RA 2,266,195 C→T 22.2% P83P (CCG→CCA tufB ← protein chain elongation factor EF‑Tu
RA 2,266,204 T→A 13.9% V80V (GTA→GTT tufB ← protein chain elongation factor EF‑Tu
RA 2,314,904 A→G 7.6% Q74Q (CAA→CAG glpK → glycerol kinase
RA 2,343,092 C→T 24.3% H273H (CAC→CAT glnL → sensory kinase in two‑component regulatory system with GlnG
RA 2,359,867 G→A 45.3% noncoding (1228/2905 nt) rrlA ← 23S ribosomal RNA
RA 2,359,876 C→T 27.6% noncoding (1219/2905 nt) rrlA ← 23S ribosomal RNA
RA 2,385,964 A→T 13.4% L56L (CTA→CTT metR → DNA‑binding transcriptional activator, homocysteine‑binding
RA 2,396,393 T→G 11.4% V265V (GTA→GTC) ‡ corA ← magnesium/nickel/cobalt transporter
RA 2,396,394 A→G 11.7% V265A (GTA→GCA) ‡ corA ← magnesium/nickel/cobalt transporter
RA 2,396,396 C→G 11.9% V264V (GTG→GTC corA ← magnesium/nickel/cobalt transporter
RA 2,396,399 G→C 12.4% S263S (TCC→TCG corA ← magnesium/nickel/cobalt transporter
RA 2,396,402 T→G 9.6% V262V (GTA→GTC) ‡ corA ← magnesium/nickel/cobalt transporter
RA 2,396,403 A→G 10.6% V262A (GTA→GCA) ‡ corA ← magnesium/nickel/cobalt transporter
RA 2,396,417 T→C 9.5% K257K (AAA→AAG corA ← magnesium/nickel/cobalt transporter
RA 2,396,426 G→C 9.3% R254R (CGC→CGG) ‡ corA ← magnesium/nickel/cobalt transporter
RA 2,396,427 C→T 9.2% R254H (CGC→CAC) ‡ corA ← magnesium/nickel/cobalt transporter
RA 2,396,428 G→T 10.1% R254S (CGC→AGC) ‡ corA ← magnesium/nickel/cobalt transporter
RA 2,396,435 C→G 8.8% E251D (GAG→GAC) ‡ corA ← magnesium/nickel/cobalt transporter
RA 2,396,437 C→T 9.1% E251K (GAG→AAG) ‡ corA ← magnesium/nickel/cobalt transporter
RA 2,446,556 A→C 8.8% pseudogene (1500/1647 nt) ilvG ← Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
RA 2,447,032 C→A 27.7% pseudogene (1024/1647 nt) ilvG ← Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
RA 2,494,739 G→A 5.6% P74P (CCG→CCA ibpB → heat shock chaperone
RA 2,518,526 T→C 16.0% E183E (GAA→GAG spoT ← bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 2,526,132 T→C 6.5% A142A (GCT→GCC pyrE → orotate phosphoribosyltransferase
RA 2,526,156 A→G 6.6% V150V (GTA→GTG pyrE → orotate phosphoribosyltransferase
RA 2,539,542 A→G 5.0% N23D (AAT→GAT)  rfaJ → UDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase
RA 2,545,256 C→T 5.9% A81T (GCG→ACG)  rfaC ← ADP‑heptose:LPS heptosyl transferase I
RA 2,545,260 A→G 5.1% R79R (CGT→CGC) ‡ rfaC ← ADP‑heptose:LPS heptosyl transferase I
RA 2,545,261 C→T 5.2% R79H (CGT→CAT) ‡ rfaC ← ADP‑heptose:LPS heptosyl transferase I
RA 2,545,265 A→G 5.7% F78L (TTT→CTT)  rfaC ← ADP‑heptose:LPS heptosyl transferase I
RA 2,595,746 G→A 7.5% I284I (ATC→ATT arsB ← arsenite/antimonite transporter
RA 2,595,749 G→A 6.8% V283V (GTC→GTT arsB ← arsenite/antimonite transporter
RA 2,595,752 A→G 7.9% I282I (ATT→ATC arsB ← arsenite/antimonite transporter
RA 2,595,761 G→T 12.0% P279P (CCC→CCA arsB ← arsenite/antimonite transporter
RA 2,595,764 G→C 12.0% A278A (GCC→GCG arsB ← arsenite/antimonite transporter
RA 2,595,782 A→T 13.6% G272G (GGT→GGA arsB ← arsenite/antimonite transporter
RA 2,595,785 C→G 12.6% T271T (ACG→ACC arsB ← arsenite/antimonite transporter
RA 2,595,788 A→G 12.2% N270N (AAT→AAC arsB ← arsenite/antimonite transporter
RA 2,595,791 A→G 11.1% I269I (ATT→ATC arsB ← arsenite/antimonite transporter
RA 2,595,796 C→A 12.0% A268S (GCG→TCG)  arsB ← arsenite/antimonite transporter
RA 2,611,527 A→G 9.7% L141P (CTT→CCT)  nikB ← nickel transporter subunit
RA 2,682,413 G→A 15.5% F433F (TTC→TTT yhgF ← predicted transcriptional accessory protein
RA 2,682,416 C→T 14.4% E432E (GAG→GAA yhgF ← predicted transcriptional accessory protein
RA 2,682,425 A→T 14.8% A429A (GCT→GCA yhgF ← predicted transcriptional accessory protein
RA 2,714,529 T→C 5.8% intergenic (‑169/+93) nirB ← / ← tsgA nitrite reductase, large subunit, NAD(P)H‑binding/predicted transporter
RA 2,749,136 G→C 8.3% G403G (GGG→GGC secY → preprotein translocase membrane subunit
RA 2,749,139 C→G 8.5% T404T (ACC→ACG secY → preprotein translocase membrane subunit
RA 2,749,142 A→G 8.2% S405S (TCA→TCG secY → preprotein translocase membrane subunit
RA 2,749,145 G→C 7.7% L406L (CTG→CTC secY → preprotein translocase membrane subunit
RA 2,749,148 T→G 6.9% L407L (CTT→CTG secY → preprotein translocase membrane subunit
RA 2,749,152 G→A 7.4% V409I (GTT→ATT) ‡ secY → preprotein translocase membrane subunit
RA 2,749,154 T→C 7.7% V409V (GTT→GTC) ‡ secY → preprotein translocase membrane subunit
RA 2,749,157 T→G 7.5% V410V (GTT→GTG secY → preprotein translocase membrane subunit
RA 2,749,160 C→G 6.8% V411V (GTC→GTG secY → preprotein translocase membrane subunit
RA 2,749,165 T→C 6.9% I413T (ATT→ACT) ‡ secY → preprotein translocase membrane subunit
RA 2,749,166 T→G 6.8% I413M (ATT→ATG) ‡ secY → preprotein translocase membrane subunit
RA 2,749,175 T→C 7.6% F416F (TTT→TTC secY → preprotein translocase membrane subunit
RA 2,749,181 T→A 9.7% A418A (GCT→GCA secY → preprotein translocase membrane subunit
RA 2,761,558 T→C 21.8% L124L (CTT→CTC aroE → dehydroshikimate reductase, NAD(P)‑binding
RA 2,761,561 C→T 24.8% I125I (ATC→ATT aroE → dehydroshikimate reductase, NAD(P)‑binding
RA 2,780,573 A→C 8.9% P516P (CCA→CCC metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
RA 2,780,576 A→G 8.6% E517E (GAA→GAG metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
RA 2,780,579 T→C 8.3% D518D (GAT→GAC metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
RA 2,807,677 T→A 5.7% intergenic (+496/‑611) tyrB → / → aphA tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent/acid phosphatase/phosphotransferase, class B, non‑specific
RA 2,810,028 T→C 15.0% P906P (CCA→CCG uvrA ← ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC
RA 2,818,696 C→T 6.5% V112V (GTC→GTT nrfB → nitrite reductase, formate‑dependent, penta‑heme cytochrome c
RA 2,859,163 A→G 7.8% L30L (CTA→CTG mutL → methyl‑directed mismatch repair protein
RA 2,880,895 C→T 8.4% L71L (TTG→TTA ytfF ← predicted inner membrane protein
RA 2,935,934 A→G 6.4% intergenic (‑144/‑73) idnD ← / → idnK L‑idonate 5‑dehydrogenase, NAD‑binding/D‑gluconate kinase, thermosensitive
RA 2,950,841 T→C 7.5% L113S (TTA→TCA)  bglJ → DNA‑binding transcriptional regulator
RA 2,965,079 T→C 11.3% I187I (ATT→ATC deoA → thymidine phosphorylase
RA 2,965,083 G→T 10.0% A189S (GCG→TCG) ‡ deoA → thymidine phosphorylase
RA 2,965,085 G→C 10.8% A189A (GCG→GCC) ‡ deoA → thymidine phosphorylase
RA 2,965,091 A→G 10.4% K191K (AAA→AAG deoA → thymidine phosphorylase
RA 2,965,094 T→G 8.2% L192L (CTT→CTG deoA → thymidine phosphorylase
RA 2,965,095 G→T 6.7% A193S (GCG→TCG)  deoA → thymidine phosphorylase
RA 2,965,099 A→C 6.9% E194A (GAA→GCA) ‡ deoA → thymidine phosphorylase
RA 2,965,100 A→G 6.9% E194E (GAA→GAG) ‡ deoA → thymidine phosphorylase
RA 2,965,103 T→C 7.0% G195G (GGT→GGC deoA → thymidine phosphorylase
RA 2,965,109 C→T 7.4% D197D (GAC→GAT deoA → thymidine phosphorylase
RA 2,965,130 A→G 11.1% K204K (AAA→AAG deoA → thymidine phosphorylase
RA 2,969,927 G→A 17.5% R161C (CGC→TGC)  lplA ← lipoate‑protein ligase A

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ Exported 252012 252032 21 10 [7] [7] 14 malZ maltodextrin glucosidase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? Exported 183922 =64 (0.870)68 (1.110)
+TTATAACA
14/80 NT 59.5% intergenic (+8/‑29) rnhB/dnaE ribonuclease HII, degrades RNA of DNA‑RNA hybrids/DNA polymerase III alpha subunit
?Exported = 296824 47 (0.640)intergenic (+125/‑28) cof/ybaO thiamin pyrimidine pyrophosphate hydrolase/predicted DNA‑binding transcriptional regulator
* ? Exported = 21562256 (0.760)191 (5.950)
+27 bp
41/42 NT 87.5% coding (732/732 nt) dnaQ DNA polymerase III epsilon subunit
?Exported 2705182 = 69 (0.940)intergenic (+106/‑1) damX/dam hypothetical protein/DNA adenine methylase
* ? Exported = 24224995 (1.300)9 (0.130) 3/92 NT 9.0% coding (1974/3147 nt) sbcC exonuclease, dsDNA, ATP‑dependent
?Exported = 242319 92 (1.310)coding (1904/3147 nt) sbcC exonuclease, dsDNA, ATP‑dependent
* ? Exported 436259 =70 (0.950)342 (5.090) 62/88 NT 87.6% intergenic (‑156/‑29) ybfF/seqA conserved hypothetical protein/regulatory protein for replication initiation
?Exported = 2706018 33 (0.490)coding (836/837 nt) dam DNA adenine methylase
* ? Exported = 43683383 (1.130)559 (7.620) 72/96 NT 84.1% coding (546/546 nt) seqA regulatory protein for replication initiation
?Exported 1803907 = 128 (1.750)intergenic (+63/‑28) ygaH/mprA predicted inner membrane protein/DNA‑binding transcriptional regulator