Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
281,512 |
C→T |
11.8% |
S11F (TCC→TTC) |
yagE → |
predicted lyase/synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 281,512 | 0 | C | T | 11.8%
| 171.4
/ 11.2
| 59 | S11F (TCC→TTC) | yagE | predicted lyase/synthase |
| Reads supporting (aligned to +/- strand): ref base C (25/27); new base T (3/4); total (28/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATAA > W3110S.gb/281445‑281578
|
taaataaaGGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCcgc > 1:3613322/1‑71 (MQ=255)
taaataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCcgc > 1:1691631/1‑71 (MQ=255)
taaataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCcgc > 1:2919167/1‑71 (MQ=255)
aaataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCcgcg > 1:3197224/1‑71 (MQ=255)
aataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCcgc < 1:1080154/69‑1 (MQ=255)
ataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCACAGTTCGCgtt < 1:1421026/71‑1 (MQ=255)
aaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCgt < 1:3908911/68‑1 (MQ=255)
cgCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCgttgtt > 1:3626228/1‑65 (MQ=255)
cgCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACgg > 1:331308/1‑70 (MQ=255)
cTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGaa < 1:155668/71‑1 (MQ=255)
tGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTTCGCGTTGTTCACgg > 1:885957/1‑67 (MQ=255)
gTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTTCGCgttg < 1:3351979/59‑1 (MQ=255)
ttCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTTCGCGTTGTTCACGGGAAt < 1:1845406/69‑1 (MQ=255)
ttCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTTCGCGTTGTTCACGGGAATc > 1:1859998/1‑70 (MQ=255)
ttCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAAt < 1:1046932/69‑1 (MQ=255)
ttCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAAt < 1:2054795/69‑1 (MQ=255)
ttCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCa > 1:2507916/1‑71 (MQ=255)
ttCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCa < 1:933432/71‑1 (MQ=255)
tCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCgttgt < 1:4102343/58‑1 (MQ=255)
cTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCAtt > 1:2138297/1‑71 (MQ=255)
taATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTccc > 1:1423088/1‑71 (MQ=255)
taATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTccc > 1:2401955/1‑71 (MQ=255)
taATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTc < 1:4372927/69‑1 (MQ=255)
tAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTc < 1:355348/51‑1 (MQ=255)
aGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGtc > 1:2155554/1‑71 (MQ=255)
aaCAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAAt < 1:1452464/56‑1 (MQ=255)
aCAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTTCGCGTTGTTCa > 1:139529/1‑48 (MQ=255)
cAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAAt < 1:2820654/54‑1 (MQ=255)
aaaaTGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTccac < 1:4189523/71‑1 (MQ=255)
aaaTGATTCAGCAAGGAGATCTCATGCCGCAGTCcg > 1:2642190/1‑36 (MQ=255)
aaTGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCgttgtt > 1:95657/1‑42 (MQ=255)
aTGATTCAGCAAGGAGATCTCATGCCGGAGTCCGCGTTGTTCACGGGAAt > 1:2591264/1‑50 (MQ=255)
aTGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCAt < 1:2199629/71‑1 (MQ=255)
aTGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCAt < 1:717815/71‑1 (MQ=255)
aTTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCAttt < 1:740884/70‑1 (MQ=255)
ttCAGCAAGGAGATCTCATGCCGCAGTCCGCgttgtt < 1:70920/37‑1 (MQ=255)
ttCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCAttttt < 1:1568424/71‑1 (MQ=255)
ttCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCAttttt < 1:904761/71‑1 (MQ=255)
cAGCAACGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTa < 1:4497144/70‑1 (MQ=255)
aGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTAc < 1:4072785/70‑1 (MQ=255)
cAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCAc < 1:3076705/35‑1 (MQ=255)
aaGGAGATCTCATGCCGCAGTTCGCGTTGTTCACgg < 1:1412345/36‑1 (MQ=255)
tctcATGCCGCAGTCCGCGTTGTTCACGGGAATCATTccccc > 1:1002903/1‑42 (MQ=255)
ctcATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGt > 1:1283736/1‑44 (MQ=255)
ctcATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGt > 1:724924/1‑44 (MQ=255)
ctcATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCAttt < 1:1632184/55‑1 (MQ=255)
ctcATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACg > 1:4493222/1‑67 (MQ=255)
ctcATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGc > 1:1540384/1‑69 (MQ=255)
tcATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAg > 1:3835540/1‑71 (MQ=255)
cATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGc > 1:4130962/1‑71 (MQ=255)
aTGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGcc > 1:1003678/1‑67 (MQ=255)
aTGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCt > 1:1951829/1‑71 (MQ=255)
tGCAGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTATTACCGCCGACGGCCAGCt > 1:3365158/1‑70 (MQ=255)
ccGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTCg < 1:3168566/70‑1 (MQ=255)
ccGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTCGa < 1:31751/71‑1 (MQ=255)
cGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTCGa > 1:364107/1‑70 (MQ=255)
cAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATaa < 1:4422964/71‑1 (MQ=255)
tCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTcca < 1:2282677/37‑1 (MQ=255)
tCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCAttt < 1:3408091/43‑1 (MQ=255)
|
TAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATAA > W3110S.gb/281445‑281578
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
