breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 58,662 G→A 24.6% V63V (GTG→GTA yabP → hypothetical protein
RA 70,434 C→T 57.1% N16N (AAC→AAT araC → DNA‑binding transcriptional dual regulator
RA 70,581 C→T 44.6% V65V (GTC→GTT araC → DNA‑binding transcriptional dual regulator
RA 70,740 T→G 56.3% G118G (GGT→GGG araC → DNA‑binding transcriptional dual regulator
RA 71,079 C→T 48.3% R231R (CGC→CGT araC → DNA‑binding transcriptional dual regulator
RA 71,082 T→C 48.2% I232I (ATT→ATC araC → DNA‑binding transcriptional dual regulator
RA 71,085 T→C 46.4% S233S (AGT→AGC araC → DNA‑binding transcriptional dual regulator
RA 71,109 T→C 46.5% T241T (ACT→ACC araC → DNA‑binding transcriptional dual regulator
RA 71,175 A→G 53.6% R263R (CGA→CGG araC → DNA‑binding transcriptional dual regulator
RA 71,214 T→C 51.1% F276F (TTT→TTC araC → DNA‑binding transcriptional dual regulator
RA 155,463 A→T 100% *247K (TAA→AAA)  ecpD ← predicted periplasmic pilin chaperone
RA 236,101 A→C 59.5% D12A (GAT→GCT)  dnaQ → DNA polymerase III epsilon subunit
RA 236,107 A→C 60.1% E14A (GAA→GCA)  dnaQ → DNA polymerase III epsilon subunit
RA 241,172 C→T 15.4% G711D (GGT→GAT)  fadE ← acyl coenzyme A dehydrogenase
RA 267,502 G→T 12.4% P228H (CCT→CAT)  ykfA ← predicted GTP‑binding protein
RA 275,785 G→A 10.0% V413I (GTA→ATA)  mmuP → predicted S‑methylmethionine transporter
RA 281,512 C→T 11.8% S11F (TCC→TTC)  yagE → predicted lyase/synthase
RA 285,498 G→A 11.9% A294T (GCC→ACC)  yagG → predicted sugar transporter
RA 323,778 G→A 5.3% intergenic (+101/+142) ykgG → / ← ykgH predicted transporter/predicted inner membrane protein
RA 345,099 C→T 17.5% A155T (GCC→ACC)  yahN ← neutral amino‑acid efflux system
RA 349,140 C→T 12.5% intergenic (+344/‑96) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 406,395 C→T 13.5% intergenic (+1/‑257) yaiA → / → aroM hypothetical protein/conserved hypothetical protein
RA 429,850 T→C 10.2% Y8H (TAT→CAT)  yajD → conserved hypothetical protein
RA 459,715 A→G 16.2% Y535C (TAC→TGC)  lon → DNA‑binding ATP‑dependent protease La
RA 464,946 A→G 10.0% F531L (TTC→CTC)  ybaE ← predicted transporter subunit
RA 547,694 A→G 100% pseudogene (114/261 nt)
pseudogene (114/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 547,831:1 +G 100% pseudogene (251/261 nt)
pseudogene (251/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 556,858 A→T 100% L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase
RA 647,863 A→G 8.4% M311T (ATG→ACG)  citF ← citrate lyase, citrate‑ACP transferase (alpha) subunit
MC JC 654,214 Δ1,199 bp 100% [dcuC][dcuC] [dcuC], insH, [dcuC]
RA 695,215 C→T 10.9% A55T (GCT→ACT)  miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 702,273 C→T 14.6% D301N (GAT→AAT)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 704,757 G→A 8.5% R131H (CGC→CAC)  nagE → fused N‑acetyl glucosamine specific PTS enzyme IICBA components
RA 711,161 C→T 13.7% intergenic (‑93/+196) fur ← / ← fldA DNA‑binding transcriptional dual regulator/flavodoxin 1
RA 833,307 G→T 12.2% T22T (ACC→ACA ybiA ← conserved hypothetical protein
RA 861,111 G→A 10.4% L640L (CTG→TTG)  ybiW ← predicted pyruvate formate lyase
RA 862,250 G→T 13.8% T260N (ACT→AAT)  ybiW ← predicted pyruvate formate lyase
RA 1,008,134 C→T 14.3% intergenic (‑112/‑132) ycbX ← / → ycbY predicted 2Fe‑2S cluster‑containing protein/predicted methyltransferase
RA 1,028,434 G→A 14.8% D23N (GAC→AAC)  yccU → predicted CoA‑binding protein with NAD(P)‑binding Rossmann‑fold domain
RA 1,055,413 A→G 20.0% D396D (GAT→GAC torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,074,318 G→T 8.7% A39E (GCG→GAG)  ycdM ← predicted monooxygenase
RA 1,084,947 A→G 18.0% K384E (AAA→GAA)  ycdB → conserved hypothetical protein
RA 1,093,686 T→C 100% V130A (GTA→GCA)  ycdT → predicted diguanylate cyclase
RA 1,099,547 C→T 10.5% A143V (GCC→GTC)  ycdX → predicted zinc‑binding hydrolase
RA 1,112,202 T→C 18.2% F431L (TTT→CTT)  mdoG → glucan biosynthesis protein, periplasmic
RA 1,115,786 T→C 7.7% intergenic (‑28/+55) msyB ← / ← mdtG hypothetical protein/predicted drug efflux system
RA 1,168,595 G→T 8.0% A312S (GCT→TCT)  ndh → respiratory NADH dehydrogenase 2/cupric reductase
RA 1,199,872 T→C 10.9% intergenic (‑58/‑400) ymfE ← / → lit predicted inner membrane protein/cell death peptidase, inhibitor of T4 late gene expression
RA 1,224,919 G→A 7.5% intergenic (‑423/‑123) ymgG ← / → ymgH hypothetical protein/hypothetical protein
RA 1,255,918 C→T 12.5% G538S (GGC→AGC)  ycgV ← predicted adhesin
RA 1,295,635 T→C 11.9% Y178H (TAC→CAC)  tdk → thymidine kinase/deoxyuridine kinase
MC JC 1,301,041 Δ1,336 bp 100% insCinsD insC, insD
RA 1,303,631 C→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit
RA 1,303,633 G→T 100% Q246Y (CAG→TAT oppA → oligopeptide transporter subunit
RA 1,364,882 G→A 5.9% V142V (GTG→GTA puuC → gamma‑Glu‑gamma‑aminobutyraldehyde dehydrogenase, NAD(P)H‑dependent
RA 1,389,997 C→T 11.3% intergenic (+22/+22) tyrR → / ← tpx DNA‑binding transcriptional dual regulator, tyrosine‑binding/lipid hydroperoxide peroxidase
RA 1,389,998 A→T 11.1% intergenic (+23/+21) tyrR → / ← tpx DNA‑binding transcriptional dual regulator, tyrosine‑binding/lipid hydroperoxide peroxidase
RA 1,389,999 A→G 11.4% intergenic (+24/+20) tyrR → / ← tpx DNA‑binding transcriptional dual regulator, tyrosine‑binding/lipid hydroperoxide peroxidase
RA 1,393,837 C→T 12.1% R45* (CGA→TGA)  ycjZ → predicted DNA‑binding transcriptional regulator
RA 1,413,523 G→A 15.2% S47F (TCC→TTC)  ydaO ← predicted C32 tRNA thiolase
RA 1,559,619 A→G 16.7% L45S (TTA→TCA)  ddpF ← D‑Ala‑D‑Ala transporter subunit
RA 1,590,693 A→G 8.9% L341L (CTT→CTC ydeT ← hypothetical protein
RA 1,683,257 G→A 17.1% D190N (GAT→AAT)  folM → dihydrofolate reductase isozyme
RA 1,691,217 G→A 10.4% A310T (GCA→ACA)  manA → mannose‑6‑phosphate isomerase
RA 1,872,074 T→C 8.0% intergenic (+122/‑25) yeaH → / → yeaI conserved hypothetical protein/predicted diguanylate cyclase
RA 2,082,018 A→G 13.1% I237I (ATT→ATC yeeA ← conserved inner membrane protein
RA 2,176,396 C→T 10.0% M6I (ATG→ATA gatC ← galactitol‑specific enzyme IIC component of PTS
MC JC 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]
MC JC 2,320,795 Δ1,336 bp 100% insD[rcsC] insD, insC, [rcsC]
RA 2,358,400 G→A 16.4% A362T (GCT→ACT)  glpA → sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding
RA 2,462,102 A→G 9.6% F53L (TTC→CTC)  sixA ← phosphohistidine phosphatase
RA 2,493,033 C→T 6.5% Q1072* (CAG→TAG)  evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA
RA 2,587,162 G→A 10.2% E304E (GAG→GAA acrD → aminoglycoside/multidrug efflux system
RA 2,622,878 T→C 6.5% G393G (GGT→GGC ppk → polyphosphate kinase, component of RNA degradosome
RA 2,632,686 A→G 7.4% V14A (GTT→GCT)  guaB ← IMP dehydrogenase
RA 2,688,553 A→T 7.8% V401D (GTC→GAC)  yfhK ← predicted sensory kinase in two‑component system
RA 2,719,037 G→A 17.1% V143V (GTG→GTA yfiQ → fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit
RA 2,771,105 G→A 11.5% N79N (AAC→AAT ypjL ← predicted inner membrane protein
RA 2,809,581 G→A 54.8% G52G (GGG→GGA mprA → DNA‑binding transcriptional regulator
RA 2,863,059 G→A 11.5% L56L (CTG→CTA ygbM → conserved hypothetical protein
RA 2,866,110 T→A 100% intergenic (‑20/+160) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein
RA 2,951,877 G→A 6.8% A928V (GCT→GTT)  recB ← exonuclease V (RecBCD complex), beta subunit
RA 2,966,215 T→G 17.6% K292N (AAA→AAC ptsP ← fused PEP‑protein phosphotransferase (enzyme I) of PTS system
RA 2,972,729 T→C 13.1% S648G (AGC→GGC)  aas ← fused 2‑acylglycerophospho‑ethanolamine acyl transferase and acyl‑acyl carrier protein synthetase
RA 3,001,511 T→C 12.8% I81T (ATA→ACA)  xdhB → xanthine dehydrogenase, FAD‑binding subunit
RA 3,012,950 A→G 13.8% V255A (GTG→GCG)  yqeC ← conserved hypothetical protein
RA 3,018,853 C→T 12.0% T346I (ACT→ATT)  ssnA → predicted chlorohydrolase/aminohydrolase
RA 3,059,788 C→T 12.0% L95L (CTG→TTG)  yliK → methylmalonyl‑CoA mutase
RA 3,065,341 G→T 10.1% I163I (ATC→ATA ygfI ← predicted DNA‑binding transcriptional regulator
RA 3,113,649 C→T 16.7% A1374T (GCA→ACA)  yghJ ← predicted inner membrane lipoprotein
RA 3,139,938 C→T 6.0% R2H (CGT→CAT)  hybD ← predicted maturation element for hydrogenase 2
RA 3,191,865 G→A 7.5% A116T (GCC→ACC)  yqiK → conserved hypothetical protein
RA 3,224,816 C→T 8.3% H147Y (CAT→TAT)  ebgC → cryptic beta‑D‑galactosidase, beta subunit
RA 3,244,017 G→A 9.7% Q86Q (CAG→CAA exuT → hexuronate transporter
RA 3,249,944 C→A 10.4% P89T (CCG→ACG)  yqjG → predicted S‑transferase
RA 3,253,271 C→T 11.9% V99V (GTC→GTT yhaK → predicted pirin‑related protein
RA 3,259,237 G→A 10.8% S613F (TCT→TTT)  tdcE ← pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
MC JC 3,261,134 Δ1,199 bp 100% insH[tdcD] insH, [tdcD]
RA 3,294,438 T→C 10.6% L395S (TTG→TCG)  yraM → conserved hypothetical protein
RA 3,306,434 G→A 13.5% L428L (CTG→TTG)  deaD ← ATP‑dependent RNA helicase
RA 3,332,615 T→A 11.3% intergenic (‑25/+102) yhbE ← / ← rpmA conserved inner membrane protein/50S ribosomal subunit protein L27
RA 3,439,867 T→C 10.9% intergenic (‑89/‑6) nudC ← / → rsd NADH pyrophosphatase/stationary phase protein, binds sigma 70 RNA polymerase subunit
RA 3,458,921 T→C 13.7% K6K (AAA→AAG nusG ← transcription termination factor
RA 3,472,611 G→A 15.5% G144G (GGC→GGT btuB ← vitamin B12/cobalamin outer membrane transporter
RA 3,506,673 A→G 5.2% I58I (ATT→ATC metL ← fused aspartokinase II and homoserine dehydrogenase II
RA 3,506,676 C→T 5.1% L57L (TTG→TTA metL ← fused aspartokinase II and homoserine dehydrogenase II
RA 3,521,572 G→A 14.1% L157L (CTG→CTA glpX → fructose 1,6‑bisphosphatase II
RA 3,533,883 T→C 8.2% intergenic (‑24/+125) yiiM ← / ← kdgT conserved hypothetical protein/2‑keto‑3‑deoxy‑D‑gluconate transporter
RA 3,564,985 T→C 11.1% F21L (TTT→CTT)  yihS → predicted glucosamine isomerase
RA 3,584,576 G→A 8.9% P21S (CCA→TCA)  hemN ← coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent
RA 3,639,730 T→C 6.7% K222R (AAA→AGA)  xerC ← site‑specific tyrosine recombinase
RA 3,659,825 C→T 11.3% M193I (ATG→ATA wzxE ← O‑antigen translocase
RA 3,704,876 C→T 8.2% M164I (ATG→ATA trkD ← potassium transporter
RA 3,707,036 G→A 14.5% A483T (GCC→ACC)  yieN → fused predicted transcriptional regulators
RA 3,727,747 T→C 11.7% V168V (GTT→GTC pstA → phosphate transporter subunit
RA 3,733,360 T→C 8.7% Y127H (TAC→CAC)  bglB → cryptic phospho‑beta‑glucosidase B
RA 3,735,429 G→A 13.0% A323T (GCA→ACA)  bglH → carbohydrate‑specific outer membrane porin, cryptic
RA 3,739,327 C→T 8.1% V195I (GTT→ATT)  yieH ← predicted hydrolase
RA 3,741,050 G→A 16.4% G326D (GGC→GAC)  yieG → predicted inner membrane protein
MC JC 3,742,938 Δ1,336 bp 100% insDinsC insD, insC
MC JC 3,746,913 Δ1,199 bp 100% [tnaB][tnaB] [tnaB], insH, [tnaB]
RA 3,764,664 T→C 17.5% N106D (AAT→GAT)  yidX ← predicted lipoproteinC
RA 3,778,496 C→T 8.2% pseudogene (67/639 nt) glvG → ECK3673:JW3658:b3681; predicted 6‑phospho‑beta‑glucosidase
RA 3,806,473 G→A 13.6% P198P (CCG→CCA yicI → predicted alpha‑glucosidase
RA 3,824,280 T→C 100% pseudogene (413/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,326 T→C 100% pseudogene (459/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,362 A→T 100% pseudogene (495/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,368 G→A 100% pseudogene (501/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,374 C→T 100% pseudogene (507/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,380 2 bp→AA 100% pseudogene (513‑514/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,410 T→A 100% pseudogene (543/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,514 T→C 100% pseudogene (647/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,524 T→G 100% pseudogene (657/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,533:1 +G 100% pseudogene (666/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,915,033 T→C 12.8% intergenic (+29/‑66) ysaB → / → glyQ hypothetical protein/glycine tRNA synthetase, alpha subunit
RA 3,915,241 A→G 12.3% E48G (GAG→GGG)  glyQ → glycine tRNA synthetase, alpha subunit
RA 3,936,857 G→A 7.3% D97N (GAC→AAC)  dppD → dipeptide transporter
RA 4,012,376 G→A 9.4% A500T (GCC→ACC)  rbbA → fused ribosome‑associated ATPases
RA 4,017,703 T→C 12.9% N1175D (AAC→GAC)  rhsB ← rhsB element core protein RshB
RA 4,027,859 A→G 20.0% L232L (TTG→CTG)  yhhT ← predicted inner membrane protein
RA 4,032,019 C→T 13.2% D650N (GAC→AAC)  zntA ← zinc, cobalt and lead efflux system
RA 4,083,312 G→A 12.3% G325E (GGG→GAG)  rtcB → conserved hypothetical protein
RA 4,093,241 C→A 8.6% G207C (GGT→TGT)  gntT ← gluconate transporter, high‑affinity GNT I system
RA 4,094,617 G→A 16.4% L60L (CTG→TTG)  gntY ← predicted gluconate transport associated protein
RA 4,123,875 G→A 8.0% R255H (CGC→CAC)  damX → hypothetical protein
RA 4,125,004 A→T 6.7% D167V (GAT→GTT)  dam → DNA adenine methylase
RA 4,184,957 A→T 13.3% intergenic (‑117/‑63) gspC ← / → gspA general secretory pathway component, cryptic/general secretory pathway component, cryptic
RA 4,184,959 T→A 13.4% intergenic (‑119/‑61) gspC ← / → gspA general secretory pathway component, cryptic/general secretory pathway component, cryptic
RA 4,220,648 C→T 15.6% P527S (CCA→TCA)  aceB → malate synthase A
RA 4,244,991 T→A 5.9% I286F (ATC→TTC)  xylE ← D‑xylose transporter
MC JC 4,310,365 Δ1,199 bp 100% insH[alsK] insH, [alsK]
RA 4,315,024 C→T 10.3% D213N (GAC→AAC)  alsA ← fused D‑allose transporter subunits and ATP‑binding components of ABC superfamily
RA 4,328,660 C→T 13.5% V125I (GTC→ATC)  phnD ← phosphonate/organophosphate ester transporter subunit
RA 4,333,478 G→A 9.7% R555H (CGC→CAC)  yjdA → conserved hypothetical protein with nucleoside triphosphate hydrolase domain
RA 4,350,232 G→A 11.9% R3C (CGC→TGC)  yjdF ← conserved inner membrane protein
RA 4,371,273 Δ2 bp 100% intergenic (‑6/+299) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,392,090 G→T 20.8% L655M (CTG→ATG)  yjeP ← predicted mechanosensitive channel
RA 4,418,872 A→G 10.8% *388* (TAA→TGA)  yjfC → predicted synthetase/amidase
RA 4,563,589 T→A 100% K207M (AAG→ATG)  iadA ← isoaspartyl dipeptidase
RA 4,586,637 G→A 14.3% P365L (CCG→CTG)  hsdM ← DNA methylase M
RA 4,591,445 G→A 7.3% T18I (ACA→ATA)  hsdR ← endonuclease R
RA 4,628,828 C→T 8.4% *215* (TGA→TAA)  ytjB ← conserved hypothetical protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 573867–575009 575999 991–2133 12 [11] [0] 124 insH–[nmpC] insH,[nmpC]
* * ÷ W3110S.gb 1421300 1421332 33 15 [11] [6] 12 ydaG/racR hypothetical protein/predicted DNA‑binding transcriptional regulator
* * ÷ W3110S.gb 2041775 2041814 40 13 [11] [10] 12 yedY predicted reductase
* * ÷ W3110S.gb 2827375 2827420 46 15 [11] [10] 12 gutM DNA‑binding transcriptional activator
* * ÷ W3110S.gb 4213619–4215889 4216713–4215891 3–3095 12 [11] [11] 12 rrlE–[rrfE] rrlE,[rrfE]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 20490NA (NA)5 (0.110) 4/92 NT NA coding (19/276 nt) insA IS1 repressor protein InsA
?W3110S.gb = 279060 NA (NA)coding (40/276 nt) insA IS1 repressor protein InsA
* ? W3110S.gb 39915 =49 (0.980)4 (0.090) 3/92 NT 8.3% coding (472/1143 nt) caiA crotonobetaine reductase subunit II, FAD‑binding
?W3110S.gb 39938 = 44 (0.980)coding (449/1143 nt) caiA crotonobetaine reductase subunit II, FAD‑binding
* ? W3110S.gb 70061 =42 (0.840)58 (1.480)
+CGCGTACAACT
41/80 NT 58.9% intergenic (‑13/‑326) araB/araC L‑ribulokinase/DNA‑binding transcriptional dual regulator
?W3110S.gb 236044 = 61 (1.220)intergenic (‑42/‑23) rnhA/dnaQ ribonuclease HI, degrades RNA of DNA‑RNA hybrids/DNA polymerase III epsilon subunit
* ? W3110S.gb = 7126553 (1.060)33 (0.910)
+AGGGTCCCTAAGTC
19/74 NT 53.2% coding (879/879 nt) araC DNA‑binding transcriptional dual regulator
?W3110S.gb 3464879 = 27 (0.540)intergenic (‑255/+46) murB/rrfB UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding/5S ribosomal RNA
* ? W3110S.gb 223991 =NA (NA)4 (0.090) 4/92 NT NA noncoding (221/1542 nt) rrsH 16S ribosomal RNA
?W3110S.gb 224012 = NA (NA)noncoding (242/1542 nt) rrsH 16S ribosomal RNA
* ? W3110S.gb 224057 =NA (NA)3 (0.060) 3/100 NT NA noncoding (287/1542 nt) rrsH 16S ribosomal RNA
?W3110S.gb 224084 = NA (NA)noncoding (314/1542 nt) rrsH 16S ribosomal RNA
* ? W3110S.gb = 224090NA (NA)4 (0.090) 3/92 NT NA noncoding (320/1542 nt) rrsH 16S ribosomal RNA
?W3110S.gb = 224102 NA (NA)noncoding (332/1542 nt) rrsH 16S ribosomal RNA
* ? W3110S.gb = 225281NA (NA)5 (0.110) 5/92 NT NA noncoding (1511/1542 nt) rrsH 16S ribosomal RNA
?W3110S.gb = 225293 NA (NA)noncoding (1523/1542 nt) rrsH 16S ribosomal RNA
* ? W3110S.gb = 225915NA (NA)9 (0.210) 6/86 NT NA noncoding (157/2904 nt) rrlH 23S ribosomal RNA
?W3110S.gb = 225926 NA (NA)noncoding (168/2904 nt) rrlH 23S ribosomal RNA
* ? W3110S.gb 228057 =NA (NA)3 (0.070) 3/92 NT NA noncoding (2299/2904 nt) rrlH 23S ribosomal RNA
?W3110S.gb 228076 = NA (NA)noncoding (2318/2904 nt) rrlH 23S ribosomal RNA
* ? W3110S.gb = 23679850 (1.000)25 (1.060)
+27 bp
21/48 NT 47.6% coding (732/732 nt) dnaQ DNA polymerase III epsilon subunit
?W3110S.gb 4124504 = 67 (1.340)intergenic (+106/‑1) damX/dam hypothetical protein/DNA adenine methylase
* ? W3110S.gb = 25173036 (0.720)3 (0.070) 3/92 NT 8.3% coding (833/1056 nt) dinB DNA polymerase IV
?W3110S.gb = 251736 34 (0.750)coding (839/1056 nt) dinB DNA polymerase IV
* ? W3110S.gb = 274373NA (NA)57 (1.140) 38/102 NT 100% intergenic (‑32/‑176) insH/mmuP IS5 transposase and trans‑activator/predicted S‑methylmethionine transporter
?W3110S.gb 576000 = 0 (0.000)pseudogene (109/1128 nt) nmpC DLP12 prophage region; ECK0544:JW5078:b0553; truncated outer membrane porin
* ? W3110S.gb 314453 =42 (0.840)85 (1.810)
+TCA
49/96 NT 81.1% coding (873/888 nt) eaeH attaching and effacing protein, pathogenesis factor
?W3110S.gb = 3920281 0 (0.000)coding (88/213 nt) cspA major cold shock protein
* ? W3110S.gb 314453 =42 (0.840)52 (1.080)
+TC
40/98 NT 71.6% coding (873/888 nt) eaeH attaching and effacing protein, pathogenesis factor
?W3110S.gb 3932930 = 1 (0.020)coding (219/1608 nt) dppA dipeptide transporter
* ? W3110S.gb = 314744NA (NA)4 (0.090) 3/88 NT NA coding (239/309 nt) insE IS3 element protein
?W3110S.gb = 314761 NA (NA)coding (256/309 nt) insE IS3 element protein
* ? W3110S.gb = 315707NA (NA)54 (1.080) 37/102 NT 100% coding (654/687 nt) ykgA predicted DNA‑binding transcriptional regulator
?W3110S.gb 576911 = 0 (0.000)coding (76/498 nt) ybcS predicted lysozyme
* ? W3110S.gb = 31684346 (0.920)4 (0.090) 3/90 NT 8.5% intergenic (‑483/+107) ykgA/ykgB predicted DNA‑binding transcriptional regulator/conserved inner membrane protein
?W3110S.gb = 316852 46 (1.040)intergenic (‑492/+98) ykgA/ykgB predicted DNA‑binding transcriptional regulator/conserved inner membrane protein
* ? W3110S.gb 324679 =38 (0.760)4 (0.080) 4/98 NT 10.3% intergenic (‑91/+122) ykgH/betA predicted inner membrane protein/choline dehydrogenase, a flavoprotein
?W3110S.gb 324704 = 33 (0.690)intergenic (‑116/+97) ykgH/betA predicted inner membrane protein/choline dehydrogenase, a flavoprotein
* ? W3110S.gb = 380919NA (NA)3 (0.070) 3/94 NT NA coding (390/411 nt)
coding (22/906 nt)
insC
insD
IS2 insertion element repressor InsA
IS2 insertion element transposase InsAB'
?W3110S.gb = 380937 NA (NA)coding (408/411 nt)
coding (40/906 nt)
insC
insD
IS2 insertion element repressor InsA
IS2 insertion element transposase InsAB'
* ? W3110S.gb 381043 =NA (NA)4 (0.090) 3/88 NT NA coding (146/906 nt) insD IS2 insertion element transposase InsAB'
?W3110S.gb 381065 = NA (NA)coding (168/906 nt) insD IS2 insertion element transposase InsAB'
* ? W3110S.gb 381129 =NA (NA)5 (0.110) 5/90 NT NA coding (232/906 nt) insD IS2 insertion element transposase InsAB'
?W3110S.gb 381154 = NA (NA)coding (257/906 nt) insD IS2 insertion element transposase InsAB'
* ? W3110S.gb 390073 =70 (1.400)7 (0.160) 4/88 NT 10.7% coding (599/1461 nt) yaiT hypothetical protein
?W3110S.gb 390095 = 56 (1.300)coding (621/1461 nt) yaiT hypothetical protein
* ? W3110S.gb 566000 =NA (NA)72 (1.440) 48/102 NT 100% intergenic (+90/‑56) ybcD/insE DLP12 prophage region; ECK0532:JW0527:b4508; predicted replication protein fragment/IS3 element protein InsE
?W3110S.gb = 576913 0 (0.000)coding (78/498 nt) ybcS predicted lysozyme
* ? W3110S.gb 616993 =55 (1.100)4 (0.090) 3/90 NT 8.6% coding (3614/3882 nt) entF enterobactin synthase multienzyme complex component, ATP‑dependent
?W3110S.gb 617023 = 36 (0.820)coding (3644/3882 nt) entF enterobactin synthase multienzyme complex component, ATP‑dependent
* ? W3110S.gb = 66384439 (0.780)4 (0.090) 4/88 NT 9.7% coding (542/1212 nt) dacA D‑alanyl‑D‑alanine carboxypeptidase
?W3110S.gb = 663852 41 (0.950)coding (534/1212 nt) dacA D‑alanyl‑D‑alanine carboxypeptidase
* ? W3110S.gb 713380 =34 (0.680)52 (1.130) 37/94 NT 63.3% intergenic (‑156/‑29) ybfF/seqA conserved hypothetical protein/regulatory protein for replication initiation
?W3110S.gb = 4125340 29 (0.630)coding (836/837 nt) dam DNA adenine methylase
* ? W3110S.gb = 71395449 (0.980)81 (1.620) 46/102 NT 61.6% coding (546/546 nt) seqA regulatory protein for replication initiation
?W3110S.gb 2809398 = 52 (1.040)intergenic (+63/‑28) ygaH/mprA predicted inner membrane protein/DNA‑binding transcriptional regulator
* ? W3110S.gb 820610 =57 (1.140)3 (0.070) 3/92 NT 5.2% coding (305/705 nt) ybhL predicted inner membrane protein
?W3110S.gb 820629 = 59 (1.310)coding (324/705 nt) ybhL predicted inner membrane protein
* ? W3110S.gb = 101650638 (0.760)4 (0.090) 3/88 NT 9.7% coding (387/519 nt) fabA beta‑hydroxydecanoyl thioester dehydrase
?W3110S.gb = 1016516 42 (0.970)coding (377/519 nt) fabA beta‑hydroxydecanoyl thioester dehydrase
* ? W3110S.gb = 115317227 (0.540)3 (0.060) 3/98 NT 9.2% intergenic (+191/‑20) fabG/acpP 3‑oxoacyl‑[acyl‑carrier‑protein] reductase/acyl carrier protein
?W3110S.gb = 1153204 33 (0.690)coding (13/237 nt) acpP acyl carrier protein
* ? W3110S.gb 1209367 =15 (0.300)26 (0.760) 22/70 NT 75.0% coding (290/630 nt) ycfK hypothetical protein
?W3110S.gb 1211196 = 7 (0.200)pseudogene (37/537 nt) stfE ECK1143:JW5172:b1157; e14 prophage region; predicted side tail fiber protein fragment
* ? W3110S.gb = 120938213 (0.260)17 (0.500) 16/70 NT 68.1% coding (305/630 nt) ycfK hypothetical protein
?W3110S.gb = 1211179 7 (0.200)pseudogene (54/537 nt) stfE ECK1143:JW5172:b1157; e14 prophage region; predicted side tail fiber protein fragment
* ? W3110S.gb 1395670 =52 (1.040)3 (0.070) 3/90 NT 6.8% coding (730/1614 nt) mppA murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
?W3110S.gb 1395691 = 36 (0.820)coding (751/1614 nt) mppA murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
* ? W3110S.gb 1481996 =31 (0.620)11 (0.230)
+AT
10/98 NT 38.2% coding (1145/1758 nt) ynbC predicted hydrolase
?W3110S.gb 2564137 = 6 (0.120)coding (1404/1404 nt) eutA reactivating factor for ethanolamine ammonia lyase
* ? W3110S.gb = 159231250 (1.000)4 (0.100) 3/84 NT 8.9% intergenic (‑62/+256) yneL/hipA predicted transcriptional regulator/regulator with hipB
?W3110S.gb = 1592319 41 (1.000)intergenic (‑69/+249) yneL/hipA predicted transcriptional regulator/regulator with hipB
* ? W3110S.gb 1942762 =32 (0.640)3 (0.070) 3/94 NT 9.2% coding (588/1323 nt) yebA predicted peptidase
?W3110S.gb 1942786 = 30 (0.650)coding (564/1323 nt) yebA predicted peptidase
* ? W3110S.gb 2018571 =4 (0.080)29 (0.620) 24/96 NT 88.5% coding (567/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb 2519723 = NA (NA)intergenic (+33/‑54) nupC/insL nucleoside (except guanosine) transporter/predicted transposase
* ? W3110S.gb = 20185832 (0.040)46 (0.960) 33/98 NT 96.0% coding (579/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb = 2521059 NA (NA)intergenic (+170/+30) insL/yfeA predicted transposase/predicted diguanylate cyclase
* ? W3110S.gb 2026073 =88 (1.760)4 (0.090) 3/96 NT 5.6% intergenic (+258/‑32) fliR/rcsA flagellar export pore protein/DNA‑binding transcriptional co‑regulator with RcsB
?W3110S.gb 2026120 = 53 (1.130)coding (16/624 nt) rcsA DNA‑binding transcriptional co‑regulator with RcsB
* ? W3110S.gb 2028009 =56 (1.120)3 (0.060) 3/96 NT 5.8% coding (362/816 nt) yedP conserved hypothetical protein
?W3110S.gb 2028031 = 45 (0.960)coding (384/816 nt) yedP conserved hypothetical protein
* ? W3110S.gb = 210734846 (0.920)3 (0.070) 3/92 NT 7.5% coding (847/993 nt) wbbI conserved hypothetical protein
?W3110S.gb = 2107390 32 (0.710)coding (805/993 nt) wbbI conserved hypothetical protein
* ? W3110S.gb = 2173564NA (NA)97 (1.980) 53/100 NT 100% pseudogene (307/2041 nt)
pseudogene (307/339 nt)
gatR
gatR
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
DNA‑binding transcriptional regulator, C‑ter fragment; ECK2083:JW2074:b2090
?W3110S.gb 3920278 = 0 (0.000)coding (91/213 nt) cspA major cold shock protein
* ? W3110S.gb = 2173565NA (NA)59 (1.180) 43/102 NT 98.3% pseudogene (306/2041 nt)
pseudogene (306/339 nt)
gatR
gatR
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
DNA‑binding transcriptional regulator, C‑ter fragment; ECK2083:JW2074:b2090
?W3110S.gb = 3932933 1 (0.020)coding (222/1608 nt) dppA dipeptide transporter
* ? W3110S.gb = 219193761 (1.220)4 (0.090) 3/90 NT 6.5% coding (2308/2481 nt) yehB predicted outer membrane protein
?W3110S.gb = 2191956 61 (1.380)coding (2289/2481 nt) yehB predicted outer membrane protein
* ? W3110S.gb = 219934743 (0.860)3 (0.060) 3/96 NT 6.8% coding (1714/2034 nt) metG methionyl‑tRNA synthetase
?W3110S.gb = 2199369 42 (0.890)coding (1736/2034 nt) metG methionyl‑tRNA synthetase
* ? W3110S.gb 2340750 =64 (1.280)4 (0.090) 3/90 NT 6.8% coding (565/1689 nt) yfaA hypothetical protein
?W3110S.gb 2340779 = 54 (1.220)coding (536/1689 nt) yfaA hypothetical protein
* ? W3110S.gb 2373655 =40 (0.800)3 (0.070) 3/94 NT 6.8% coding (947/1983 nt) yfbG fused UDP‑L‑Ara4N formyltransferase and UDP‑GlcA C‑4'‑decarboxylase
?W3110S.gb 2373675 = 45 (0.980)coding (967/1983 nt) yfbG fused UDP‑L‑Ara4N formyltransferase and UDP‑GlcA C‑4'‑decarboxylase
* ? W3110S.gb = 2412225NA (NA)67 (1.340) 46/102 NT 94.4% intergenic (‑55/‑782) insA/yfbQ IS1 element protein/predicted aminotransferase
?W3110S.gb = 3748807 4 (0.080)coding (176/984 nt) tnaB tryptophan transporter of low affinity
* ? W3110S.gb = 243040132 (0.640)4 (0.090) 3/88 NT 9.7% coding (279/717 nt) hisM histidine/lysine/arginine/ornithine transporter subunit
?W3110S.gb = 2430411 47 (1.090)coding (269/717 nt) hisM histidine/lysine/arginine/ornithine transporter subunit
* ? W3110S.gb = 272286834 (0.680)3 (0.070) 3/88 NT 8.7% coding (34/273 nt) yfiM hypothetical protein
?W3110S.gb = 2722875 34 (0.790)coding (41/273 nt) yfiM hypothetical protein
* ? W3110S.gb 2727289 =NA (NA)3 (0.060) 3/96 NT NA noncoding (552/2904 nt) rrlG 23S ribosomal RNA
?W3110S.gb 2727320 = NA (NA)noncoding (521/2904 nt) rrlG 23S ribosomal RNA
* ? W3110S.gb = 27282900 (0.000)3 (0.070) 3/92 NT 100% noncoding (1524/1542 nt) rrsG 16S ribosomal RNA
?W3110S.gb = 2728302 NA (NA)noncoding (1512/1542 nt) rrsG 16S ribosomal RNA
* ? W3110S.gb = 292673438 (0.760)5 (0.120) 4/88 NT 12.9% intergenic (+406/‑151) ygdH/sdaC conserved hypothetical protein/predicted serine transporter
?W3110S.gb = 2926743 35 (0.810)intergenic (+415/‑142) ygdH/sdaC conserved hypothetical protein/predicted serine transporter
* ? W3110S.gb = 294457669 (1.380)4 (0.090) 3/88 NT 6.1% intergenic (‑78/+161) ygdL/mltA conserved hypothetical protein/membrane‑bound lytic murein transglycosylase A
?W3110S.gb 3866182 = 64 (1.480)intergenic (‑189/+41) mtlD/mtlA mannitol‑1‑phosphate dehydrogenase, NAD(P)‑binding/fused mannitol‑specific PTS enzyme IIABC components
* ? W3110S.gb = 297062949 (0.980)4 (0.090) 3/94 NT 8.1% coding (377/1041 nt) tas predicted oxidoreductase, NADP(H)‑dependent aldo‑keto reductase
?W3110S.gb = 2970640 46 (1.000)coding (388/1041 nt) tas predicted oxidoreductase, NADP(H)‑dependent aldo‑keto reductase
* ? W3110S.gb = 327190941 (0.820)3 (0.070) 3/94 NT 6.4% coding (704/891 nt) garR tartronate semialdehyde reductase
?W3110S.gb = 3271923 50 (1.090)coding (690/891 nt) garR tartronate semialdehyde reductase
* ? W3110S.gb 3389452 =49 (0.980)3 (0.060) 3/98 NT 5.5% coding (78/930 nt) aaeR predicted DNA‑binding transcriptional regulator, efflux system
?W3110S.gb 3389477 = 57 (1.190)coding (103/930 nt) aaeR predicted DNA‑binding transcriptional regulator, efflux system
* ? W3110S.gb 3589856 =40 (0.800)3 (0.070) 3/86 NT 8.9% intergenic (‑141/‑223) polA/yihG fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease/predicted endonuclease
?W3110S.gb 3589885 = 28 (0.660)intergenic (‑170/‑194) polA/yihG fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease/predicted endonuclease
* ? W3110S.gb 3628496 =53 (1.060)3 (0.060) 3/96 NT 5.1% coding (429/621 nt) rhtC threonine efflux system
?W3110S.gb 3628521 = 62 (1.320)coding (404/621 nt) rhtC threonine efflux system
* ? W3110S.gb 3748799 =6 (0.120)59 (1.180) 44/102 NT 90.8% coding (184/984 nt) tnaB tryptophan transporter of low affinity
?W3110S.gb 4056222 = NA (NA)intergenic (‑564/+15) yrhB/insB hypothetical protein/IS1 transposase InsAB'
* ? W3110S.gb = 37505920 (0.000)83 (1.800) 48/94 NT 100% intergenic (‑104/‑69) tnaA/insH tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent/IS5 element protein
?W3110S.gb 3751792 = 0 (0.000)intergenic (+151/+116) insH/tnaC IS5 element protein/tryptophanase leader peptide
* ? W3110S.gb = 393169555 (1.100)3 (0.070) 3/92 NT 5.7% intergenic (+62/‑30) eptB/proK predicted metal dependent hydrolase/tRNA‑Pro
?W3110S.gb = 3931713 50 (1.110)intergenic (+80/‑12) eptB/proK predicted metal dependent hydrolase/tRNA‑Pro
* ? W3110S.gb 4058811 =56 (1.120)3 (0.070) 3/90 NT 5.7% coding (469/489 nt) yhhY predicted acetyltransferase
?W3110S.gb 4058829 = 50 (1.130)coding (451/489 nt) yhhY predicted acetyltransferase
* ? W3110S.gb = 436625244 (0.880)3 (0.070) 3/94 NT 7.4% coding (363/1539 nt) cadC DNA‑binding transcriptional activator
?W3110S.gb = 4366279 34 (0.740)coding (336/1539 nt) cadC DNA‑binding transcriptional activator
* ? W3110S.gb 4481821 =44 (0.880)3 (0.070) 3/92 NT 8.3% coding (1703/1815 nt) yjgL hypothetical protein
?W3110S.gb 4481843 = 27 (0.600)coding (1725/1815 nt) yjgL hypothetical protein
* ? W3110S.gb = 449205460 (1.200)3 (0.070) 3/92 NT 5.1% coding (55/1083 nt) yjgQ conserved inner membrane protein
?W3110S.gb = 4492075 57 (1.260)coding (76/1083 nt) yjgQ conserved inner membrane protein
* ? W3110S.gb = 450773461 (1.220)3 (0.060) 3/98 NT 5.2% coding (380/1329 nt) insG IS4 predicted transposase
?W3110S.gb = 4507758 51 (1.060)coding (356/1329 nt) insG IS4 predicted transposase
* ? W3110S.gb 4541675 =49 (0.980)5 (0.110) 3/92 NT 10.0% coding (602/981 nt) yjhS conserved hypothetical protein
?W3110S.gb 4541699 = 46 (1.020)coding (578/981 nt) yjhS conserved hypothetical protein
* ? W3110S.gb 4547364 =20 (0.400)7 (0.170) 6/84 NT 25.2% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?W3110S.gb 4547678 = 25 (0.610)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
* ? W3110S.gb = 454737219 (0.380)6 (0.150) 5/84 NT 22.8% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?W3110S.gb = 4547668 25 (0.610)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
* ? W3110S.gb 4644534 =33 (0.660)4 (0.090) 3/90 NT 12.5% coding (455/717 nt) arcA DNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA
?W3110S.gb 4644554 = 27 (0.610)coding (435/717 nt) arcA DNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA