Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,972,729 |
T→C |
13.1% |
S648G (AGC→GGC) |
aas ← |
fused 2‑acylglycerophospho‑ethanolamine acyl transferase and acyl‑acyl carrier protein synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,972,729 | 0 | T | C | 13.1%
| 155.3
/ 14.3
| 61 | S648G (AGC→GGC) | aas | fused 2‑acylglycerophospho‑ethanolamine acyl transferase and acyl‑acyl carrier protein synthetase |
| Reads supporting (aligned to +/- strand): ref base T (30/23); new base C (5/3); total (35/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGA > W3110S.gb/2972660‑2972798
|
gCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGct < 1:1064996/70‑1 (MQ=255)
aaCTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGccct > 1:3676215/1‑70 (MQ=255)
aCTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCtt > 1:1858814/1‑70 (MQ=255)
aCTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCtt > 1:776767/1‑70 (MQ=255)
aCTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCtt > 1:782154/1‑70 (MQ=255)
tATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGccct > 1:3406812/1‑66 (MQ=255)
tATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGccct > 1:1663337/1‑66 (MQ=255)
aTCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAAt < 1:401480/69‑1 (MQ=255)
tCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCg < 1:2097213/70‑1 (MQ=255)
tCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCg > 1:2945268/1‑70 (MQ=255)
tCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGc < 1:2876330/71‑1 (MQ=255)
tCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCg < 1:4030766/70‑1 (MQ=255)
cgcgTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGt < 1:3652117/71‑1 (MQ=255)
gcgTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTg < 1:4598974/71‑1 (MQ=255)
gcgTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTg > 1:1690467/1‑71 (MQ=255)
cAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGc > 1:1754790/1‑63 (MQ=255)
cAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCAt > 1:100891/1‑71 (MQ=255)
aGTTCGTTATCTGTGTTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCAt < 1:3401501/70‑1 (MQ=255)
gTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATgg < 1:676157/71‑1 (MQ=255)
ttCGTTATCTGTGGTGAAAAGCACGAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGc < 1:44254/60‑1 (MQ=255)
ttCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCa > 1:3659348/1‑67 (MQ=255)
ttCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGa > 1:2815956/1‑71 (MQ=255)
tCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTg > 1:974871/1‑63 (MQ=255)
cGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACt > 1:236035/1‑71 (MQ=255)
cGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACt > 1:2471598/1‑71 (MQ=255)
cGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACt > 1:3774635/1‑71 (MQ=255)
ttATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGctct < 1:4391534/49‑1 (MQ=255)
aTCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTg > 1:350653/1‑58 (MQ=255)
aTCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTAt > 1:3064893/1‑71 (MQ=255)
aTCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTAt > 1:3215610/1‑71 (MQ=255)
tCTGTGGTGAAGCGCACCAGTGCCTCGCCTTTACTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATc > 1:2535308/1‑71 (MQ=255)
tggtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGAATGGACTTTATCt < 1:4443923/68‑1 (MQ=255)
gtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGa < 1:1461557/71‑1 (MQ=255)
tgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGaa < 1:934594/71‑1 (MQ=255)
gAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATg < 1:981673/54‑1 (MQ=255)
gAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGaaa > 1:1774080/1‑71 (MQ=255)
aaaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTAt > 1:2578001/1‑61 (MQ=255)
aGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCt < 1:1956455/60‑1 (MQ=255)
cACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCaa < 1:3007208/71‑1 (MQ=255)
cAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGa < 1:2837844/47‑1 (MQ=255)
cAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTg > 1:3492050/1‑71 (MQ=255)
aGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGa > 1:4548998/1‑59 (MQ=255)
gTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAaca > 1:2925521/1‑62 (MQ=255)
gTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCAGGGACTTTATCTgg > 1:2194087/1‑55 (MQ=255)
gTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGaaa > 1:3345922/1‑60 (MQ=255)
gTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGaaa > 1:1562712/1‑60 (MQ=255)
tGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCa < 1:3476092/71‑1 (MQ=255)
ccTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAg < 1:2602484/70‑1 (MQ=255)
ccTCGCCCTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTa > 1:2452517/1‑47 (MQ=255)
tCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACtt < 1:1214671/43‑1 (MQ=255)
cGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGTCAgttgt > 1:796943/1‑71 (MQ=255)
cGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAgt < 1:3308411/68‑1 (MQ=255)
gCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAgttgtt > 1:1675078/1‑71 (MQ=255)
gCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAgttgtt < 1:3223346/71‑1 (MQ=255)
gCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAgttgtt > 1:1341735/1‑71 (MQ=255)
gCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAgttgtt < 1:1042812/71‑1 (MQ=255)
ccTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTc < 1:1171417/71‑1 (MQ=255)
cTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTc > 1:626503/1‑70 (MQ=255)
tGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTTTTccac < 1:10428/70‑1 (MQ=255)
tGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTccac > 1:2524084/1‑70 (MQ=255)
gCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTccac > 1:2871461/1‑65 (MQ=255)
cGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAg < 1:4330234/70‑1 (MQ=255)
gCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGaaa > 1:1094450/1‑38 (MQ=255)
gCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGaaa > 1:1019214/1‑38 (MQ=255)
gCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGc > 1:3083458/1‑70 (MQ=255)
gCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCg < 1:3736554/71‑1 (MQ=255)
ctctTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGc < 1:1253193/47‑1 (MQ=255)
ctctTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCAtt < 1:3129551/63‑1 (MQ=255)
ctctTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGa > 1:3119504/1‑71 (MQ=255)
|
GCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGA > W3110S.gb/2972660‑2972798
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
