Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,733,360 |
T→C |
8.7% |
Y127H (TAC→CAC) |
bglB → |
cryptic phospho‑beta‑glucosidase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,733,360 | 0 | T | C | 8.7%
| 120.8
/ 4.4
| 46 | Y127H (TAC→CAC) | bglB | cryptic phospho‑beta‑glucosidase B |
| Reads supporting (aligned to +/- strand): ref base T (21/21); new base C (2/2); total (23/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 |
GCGTTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCG > W3110S.gb/3733291‑3733420
|
gCGTTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTa < 1:2024847/71‑1 (MQ=255)
cGTTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTAc > 1:4060149/1‑71 (MQ=255)
gTTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTAc > 1:389862/1‑70 (MQ=255)
gTTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTAc > 1:2130883/1‑70 (MQ=255)
ttACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCAtt > 1:3071559/1‑65 (MQ=255)
ttACGATCGGCTGTTTGATAAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGa > 1:2769759/1‑69 (MQ=255)
cGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGc > 1:1306601/1‑71 (MQ=255)
gATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGcc < 1:799538/71‑1 (MQ=255)
cTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATgg < 1:3192661/71‑1 (MQ=255)
tGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCa > 1:3799015/1‑65 (MQ=255)
tttGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGc < 1:3470931/70‑1 (MQ=255)
tgatgaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGc > 1:521134/1‑68 (MQ=255)
tgatgaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTgg > 1:3905559/1‑71 (MQ=255)
aaaTGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGaaa < 1:4127628/71‑1 (MQ=255)
aaaTGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGaaa < 1:3778175/71‑1 (MQ=255)
tGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATg > 1:4085106/1‑57 (MQ=255)
tGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAAc < 1:3858332/71‑1 (MQ=255)
gcgcAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTa > 1:539749/1‑71 (MQ=255)
gcgcAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTa > 1:3205830/1‑71 (MQ=255)
gcgcAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTa > 1:349735/1‑71 (MQ=255)
gcAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTAcg < 1:2284390/71‑1 (MQ=255)
cAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTAcgg < 1:431095/71‑1 (MQ=255)
ggCGGGGATCAAGCCGCTGGTAACGTTATCCCAtt < 1:4046944/35‑1 (MQ=255)
ggCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCa < 1:2967784/46‑1 (MQ=255)
gCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGaaa > 1:1413077/1‑40 (MQ=255)
gCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCt > 1:1170603/1‑53 (MQ=255)
gCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTAcggcgg < 1:3931567/71‑1 (MQ=255)
gCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACGa < 1:4503777/38‑1 (MQ=255)
cGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGt < 1:3317076/71‑1 (MQ=255)
ggggATCAAGCCGCTGGTAACGTTATCCCATTACGAAATg < 1:2241669/40‑1 (MQ=255)
ggggATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATg < 1:391357/47‑1 (MQ=255)
ggggATCAAGCCGCTGGTAACGTTATAACATTACGAAATg > 1:52443/1‑40 (MQ=255)
gATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGc < 1:1799309/47‑1 (MQ=255)
gATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTAc > 1:2902811/1‑61 (MQ=255)
aTCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTGGTGATAAACTACGGCGGTTGGGc > 1:720790/1‑71 (MQ=255)
tCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCt < 1:2292555/71‑1 (MQ=255)
tCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGc > 1:4379588/1‑70 (MQ=255)
aGCCGCTGGTAAGGTTATCCCATTACGAAATGCCATATGGGCTGGTGaaaaa > 1:1155024/1‑52 (MQ=255)
gCCGCTGGTAACGTTATCCCATTACGAAATGCCata > 1:3706222/1‑36 (MQ=255)
gCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATc > 1:134352/1‑71 (MQ=255)
ccGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCTGTTGGGCTAATCg < 1:4057726/71‑1 (MQ=255)
cTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAg < 1:3133239/70‑1 (MQ=255)
tAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGaaa < 1:3675733/41‑1 (MQ=255)
aaCGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCa > 1:4104870/1‑71 (MQ=255)
gTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATc > 1:4369642/1‑70 (MQ=255)
gTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCg < 1:4150782/71‑1 (MQ=255)
tCCCATTACGAAATGCCATATGGGCTTGTGAAAAAc < 1:98715/36‑1 (MQ=255)
ccATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCg < 1:1180820/65‑1 (MQ=255)
|
GCGTTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCG > W3110S.gb/3733291‑3733420
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
