Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,074,318 G→T A39E (GCG→GAG)  ycdM ← predicted monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,074,3180GT100.0% 225.2 / NA 79A39E (GCG→GAG) ycdMpredicted monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (79/0);  total (79/0)

TTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAA  >  W3110S.gb/1074269‑1074330
                                                 |            
tttttGGACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:994393/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:646002/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:458833/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:644276/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:636916/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:594814/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:572129/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:539930/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:529178/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:524584/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:522718/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:521139/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:520316/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:514313/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:505647/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:502909/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:492033/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:474285/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:462950/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:45251/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:827612/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:985619/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:947261/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:936630/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:933934/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:930282/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:898510/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:892485/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:889076/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:846127/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:705278/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:803247/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:802329/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:798098/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:764639/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:751273/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:73969/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:729909/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:709950/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1295352/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1007891/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1435138/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1417297/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1400436/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1386075/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1364550/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1358045/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1328142/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1305378/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1449637/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:128964/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:117164/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1166405/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1139521/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1099019/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1092912/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1084286/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1059993/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1049085/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:233919/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:440835/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:417761/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:394743/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:371511/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:367439/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:360376/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:25921/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:244470/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:234197/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1453129/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:233220/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:193697/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:166200/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1531299/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1520549/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1516243/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1465705/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGaaa  >  1:1460377/1‑62 (MQ=255)
tttttGCACAATGGCTTTATTCAGTTCAAACGTCGGCATGAACTGCGGCTCCTGGGTCGaaa  >  1:719254/1‑62 (MQ=255)
                                                 |            
TTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAA  >  W3110S.gb/1074269‑1074330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A