Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,074,318 |
G→T |
A39E (GCG→GAG) |
ycdM ← |
predicted monooxygenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,074,318 | 0 | G | T | 100.0%
| 88.6
/ NA
| 28 | A39E (GCG→GAG) | ycdM | predicted monooxygenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (20/8); total (20/8) |
CTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCA > W3110S.gb/1074263‑1074368
|
cTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTggg > 1:488196/1‑62 (MQ=255)
cTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTggg > 1:451201/1‑62 (MQ=255)
cTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTggg > 1:4265694/1‑62 (MQ=255)
cTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTggg > 1:3448848/1‑62 (MQ=255)
cTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTggg > 1:2627711/1‑62 (MQ=255)
tGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGa < 1:93923/61‑1 (MQ=255)
tGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGa < 1:617329/61‑1 (MQ=255)
aaTGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATTagccagc < 1:780450/62‑1 (MQ=255)
aaTGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGagccagc < 1:3520178/62‑1 (MQ=255)
aaTGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGagccagc < 1:1233811/62‑1 (MQ=255)
tCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGcc > 1:3312948/1‑61 (MQ=255)
tCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGcc > 1:3383659/1‑61 (MQ=255)
tCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGcc > 1:1207759/1‑61 (MQ=255)
tCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGcc > 1:3845563/1‑61 (MQ=255)
tCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGcc > 1:718033/1‑61 (MQ=255)
ttCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAg < 1:1163148/62‑1 (MQ=255)
ttCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAg < 1:257675/62‑1 (MQ=255)
ttCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAg < 1:4554152/62‑1 (MQ=255)
aaGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTa > 1:883664/1‑61 (MQ=255)
aaGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTa > 1:2168856/1‑61 (MQ=255)
tCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAAt > 1:24633/1‑62 (MQ=255)
tCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAAt > 1:4338562/1‑62 (MQ=255)
tCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAAt > 1:3230384/1‑62 (MQ=255)
tGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCgtt > 1:2574602/1‑37 (MQ=255)
tGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCa > 1:4586847/1‑62 (MQ=255)
tGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCa > 1:702926/1‑62 (MQ=255)
tGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCa > 1:246726/1‑62 (MQ=255)
tGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCa > 1:1130229/1‑62 (MQ=255)
|
CTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCA > W3110S.gb/1074263‑1074368
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
