breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 47,858 G→A S30S (TCG→TCA kefC → potassium:proton antiporter
RA 67,598 C→T G247D (GGC→GAC)  araA ← L‑arabinose isomerase
RA 155,463 A→T *247K (TAA→AAA)  ecpD ← predicted periplasmic pilin chaperone
RA 257,873 A→G K15K (AAA→AAG crl → DNA‑binding transcriptional regulator
RA 267,502 G→T P228H (CCT→CAT)  ykfA ← predicted GTP‑binding protein
RA 275,785 G→A V413I (GTA→ATA)  mmuP → predicted S‑methylmethionine transporter
RA 285,498 G→A A294T (GCC→ACC)  yagG → predicted sugar transporter
RA 330,092 A→G N469S (AAC→AGC)  betT → choline transporter of high affinity
RA 345,099 C→T A155T (GCC→ACC)  yahN ← neutral amino‑acid efflux system
RA 349,140 C→T intergenic (+344/‑96) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 429,850 T→C Y8H (TAT→CAT)  yajD → conserved hypothetical protein
RA 453,398 +G intergenic (‑7/‑298) yajG ← / → bolA predicted lipoprotein/regulator of penicillin binding proteins and beta lactamase transcription (morphogene)
RA 460,284 G→T A725S (GCA→TCA)  lon → DNA‑binding ATP‑dependent protease La
RA 464,946 A→G F531L (TTC→CTC)  ybaE ← predicted transporter subunit
RA 478,343 C→T A45T (GCT→ACT)  ylaC ← predicted inner membrane protein
RA 527,659 A→G K163E (AAA→GAA)  ylbH → conserved hypothetical protein, rhs‑like
RA 547,694 A→G pseudogene (114/261 nt)
pseudogene (114/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 547,835 +G pseudogene (255/261 nt)
pseudogene (255/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 548,801 G→A pseudogene (1221/1259 nt)
pseudogene (964/1002 nt)
ylbE →
ylbE →
ECK0512:JW0508+JW0507:b4572; hypothetical protein
ECK0512:JW0508:b0519; hypothetical protein, C‑ter fragment
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase
RA 563,782 C→T intergenic (‑79/‑164) fimZ ← / → argU predicted DNA‑binding transcriptional regulator/tRNA‑Arg
RA 593,201 A→G F265L (TTC→CTC)  cusS ← sensory histidine kinase in two‑component regulatory system with CusR, senses copper ions
RA 631,602 A→G *66* (TAA→TAG ybdD → conserved hypothetical protein
RA 639,164 G→A P63P (CCG→CCA ahpF → alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding
RA 647,863 A→G M311T (ATG→ACG)  citF ← citrate lyase, citrate‑ACP transferase (alpha) subunit
RA 651,069 T→C N4S (AAT→AGT)  citC ← citrate lyase synthetase
MC JC 654,214 Δ1,199 bp [dcuC][dcuC] [dcuC], insH, [dcuC]
RA 707,707 A→G N398S (AAC→AGC)  glnS → glutamyl‑tRNA synthetase
RA 711,161 C→T intergenic (‑93/+196) fur ← / ← fldA DNA‑binding transcriptional dual regulator/flavodoxin 1
RA 811,947 G→T G382V (GGC→GTC) 
A2S (GCA→TCA) 
bioF →
bioC →
8‑amino‑7‑oxononanoate synthase
predicted methltransferase, enzyme of biotin synthesis
RA 833,307 G→T T22T (ACC→ACA ybiA ← conserved hypothetical protein
RA 861,111 G→A L640L (CTG→TTG)  ybiW ← predicted pyruvate formate lyase
RA 862,250 G→T T260N (ACT→AAT)  ybiW ← predicted pyruvate formate lyase
RA 891,153 C→T R8H (CGT→CAT)  grxA ← glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a)
RA 912,695 C→T P514P (CCG→CCA hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 922,613 G→A L615L (CTG→CTA macB → fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily
RA 976,718 G→A E216E (GAG→GAA mukE → protein involved in chromosome partitioning
RA 1,007,501 A→C N174K (AAT→AAG ycbX ← predicted 2Fe‑2S cluster‑containing protein
RA 1,008,014 T→A T3T (ACA→ACT ycbX ← predicted 2Fe‑2S cluster‑containing protein
RA 1,008,134 C→T intergenic (‑112/‑132) ycbX ← / → ycbY predicted 2Fe‑2S cluster‑containing protein/predicted methyltransferase
RA 1,032,415 C→T intergenic (‑281/‑146) serT ← / → hyaA tRNA‑Ser/hydrogenase 1, small subunit
RA 1,036,407 A→G E72E (GAA→GAG hyaD → protein involved in processing of HyaA and HyaB proteins
RA 1,055,413 A→G D396D (GAT→GAC torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,074,318 G→T A39E (GCG→GAG)  ycdM ← predicted monooxygenase
RA 1,084,947 A→G K384E (AAA→GAA)  ycdB → conserved hypothetical protein
RA 1,088,483 C→T Q599Q (CAG→CAA ycdR ← predicted enzyme associated with biofilm formation
RA 1,093,686 T→C V130A (GTA→GCA)  ycdT → predicted diguanylate cyclase
RA 1,097,861 G→A noncoding (33/88 nt) serX ← tRNA‑Ser
RA 1,099,547 C→T A143V (GCC→GTC)  ycdX → predicted zinc‑binding hydrolase
RA 1,115,786 T→C intergenic (‑28/+55) msyB ← / ← mdtG hypothetical protein/predicted drug efflux system
RA 1,142,123 A→G I172V (ATA→GTA)  flgL → flagellar hook‑filament junction protein
RA 1,210,070 C→T S121F (TCC→TTC)  ymfS → hypothetical protein
RA 1,224,919 G→A intergenic (‑423/‑123) ymgG ← / → ymgH hypothetical protein/hypothetical protein
RA 1,234,526 T→C T28A (ACG→GCG)  dsbB ← oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
RA 1,254,608 T→C intergenic (+46/+54) dhaR → / ← ycgV predicted DNA‑binding transcriptional regulator, dihydroxyacetone/predicted adhesin
RA 1,255,918 C→T G538S (GGC→AGC)  ycgV ← predicted adhesin
RA 1,268,022 A→G T118A (ACG→GCG)  prmC → N5‑glutamine methyltransferase, modifies release factors RF‑1 and RF‑2
RA 1,274,854 G→A T108M (ACG→ATG)  ychN ← conserved hypothetical protein
RA 1,295,635 T→C Y178H (TAC→CAC)  tdk → thymidine kinase/deoxyuridine kinase
MC JC 1,301,041 Δ1,336 bp insCinsD insC, insD
RA 1,303,631 C→T Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit
RA 1,303,633 G→T Q246Y (CAG→TAT oppA → oligopeptide transporter subunit
RA 1,364,882 G→A V142V (GTG→GTA puuC → gamma‑Glu‑gamma‑aminobutyraldehyde dehydrogenase, NAD(P)H‑dependent
RA 1,393,837 C→T R45* (CGA→TGA)  ycjZ → predicted DNA‑binding transcriptional regulator
RA 1,394,475 (A)8→9 coding (771/900 nt) ycjZ → predicted DNA‑binding transcriptional regulator
RA 1,413,523 G→A S47F (TCC→TTC)  ydaO ← predicted C32 tRNA thiolase
RA 1,430,136 C→T pseudogene (965/1527 nt)
R102H (CGC→CAC) 
lomR →
insH ←
ECK1366:JW5884+JW5904:b4570; Rac prophage region; hypothetical protein
IS5 transposase and trans‑activator
RA 1,454,872 +C coding (485/2046 nt) maoC ← fused aldehyde dehydrogenase and enoyl‑CoA hydratase
RA 1,516,711 C→T A79V (GCG→GTG)  ydcV → predicted spermidine/putrescine transporter subunit
RA 1,516,927 A→G N151S (AAT→AGT)  ydcV → predicted spermidine/putrescine transporter subunit
RA 1,559,619 A→G L45S (TTA→TCA)  ddpF ← D‑Ala‑D‑Ala transporter subunit
RA 1,584,624 A→G intergenic (‑386/+16) ydeN ← / ← ydeO conserved hypothetical protein/predicted DNA‑binding transcriptional acfivator
RA 1,607,958 G→A G49S (GGT→AGT)  lsrF → predicted aldolase
RA 1,636,349 G→A A84V (GCG→GTG)  tfaQ ← predicted tail fibre assembly protein
RA 1,851,762 C→T G404G (GGC→GGT sppA → protease IV
RA 1,857,513 C→T G58E (GGA→GAA)  ydjG ← predicted oxidoreductase
RA 1,874,182 A→G Y143C (TAC→TGC)  yeaJ → predicted diguanylate cyclase
RA 1,878,701 C→T P27S (CCG→TCG)  yeaO → conserved hypothetical protein
RA 1,891,326 (C)5→6 coding (135/1686 nt) fadD ← acyl‑CoA synthetase
RA 1,930,964 T→A D153V (GAT→GTT)  yebE ← conserved hypothetical protein
RA 2,002,152 T→C Y124C (TAC→TGC)  fliY ← cystine transporter subunit
RA 2,099,387 T→C intergenic (+25/+71) hisI → / ← cld fused phosphoribosyl‑AMP cyclohydrolase and phosphoribosyl‑ATP pyrophosphatase/regulator of length of O‑antigen component of lipopolysaccharide chains
RA 2,176,396 C→T M6I (ATG→ATA gatC ← galactitol‑specific enzyme IIC component of PTS
MC JC 2,176,983 Δ1,199 bp [gatA][gatA] [gatA], insH, [gatA]
RA 2,238,432 T→C L356L (TTA→CTA)  yeiT → predicted oxidoreductase
RA 2,266,989 T→C intergenic (‑160/‑208) fruB ← / → setB fused fructose‑specific PTS enzyme IIA component and HPr component/lactose/glucose efflux system
MC JC 2,320,795 Δ1,336 bp insD[rcsC] insD, insC, [rcsC]
RA 2,358,400 G→A A362T (GCT→ACT)  glpA → sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding
RA 2,493,033 C→T Q1072* (CAG→TAG)  evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA
RA 2,523,255 (T)6→7 coding (24/2190 nt) yfeA ← predicted diguanylate cyclase
RA 2,792,695 C→T intergenic (+24/‑214) gabT → / → gabP 4‑aminobutyrate aminotransferase, PLP‑dependent/gamma‑aminobutyrate transporter
RA 2,841,531 G→A R57* (CGA→TGA)  hycI ← protease involved in processing C‑terminal end of HycE
RA 2,863,059 G→A L56L (CTG→CTA ygbM → conserved hypothetical protein
RA 2,866,110 T→A intergenic (‑20/+160) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein
RA 2,966,215 T→G K292N (AAA→AAC ptsP ← fused PEP‑protein phosphotransferase (enzyme I) of PTS system
RA 3,001,511 T→C I81T (ATA→ACA)  xdhB → xanthine dehydrogenase, FAD‑binding subunit
RA 3,084,437 Δ1 bp coding (131/1977 nt) speA ← biosynthetic arginine decarboxylase, PLP‑binding
RA 3,139,938 C→T R2H (CGT→CAT)  hybD ← predicted maturation element for hydrogenase 2
RA 3,191,865 G→A A116T (GCC→ACC)  yqiK → conserved hypothetical protein
RA 3,213,568 C→T intergenic (+24/+55) rpoD → / ← ygjF RNA polymerase, sigma 70 (sigma D) factor/G/U mismatch‑specific DNA glycosylase
RA 3,220,824 C→A P235T (CCG→ACG)  ebgR → DNA‑binding transcriptional repressor
RA 3,224,816 C→T H147Y (CAT→TAT)  ebgC → cryptic beta‑D‑galactosidase, beta subunit
RA 3,244,017 G→A Q86Q (CAG→CAA exuT → hexuronate transporter
RA 3,259,237 G→A S613F (TCT→TTT)  tdcE ← pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
MC JC 3,261,134 Δ1,199 bp insH[tdcD] insH, [tdcD]
RA 3,294,438 T→C L395S (TTG→TCG)  yraM → conserved hypothetical protein
RA 3,306,434 G→A L428L (CTG→TTG)  deaD ← ATP‑dependent RNA helicase
RA 3,332,615 T→A intergenic (‑25/+102) yhbE ← / ← rpmA conserved inner membrane protein/50S ribosomal subunit protein L27
RA 3,338,549 G→A F185F (TTC→TTT yrbE ← predicted toluene transporter subunit
RA 3,375,433 A→G D237G (GAT→GGT)  dcuD → predicted transporter
RA 3,459,280 T→C E15G (GAA→GGA)  secE ← preprotein translocase membrane subunit
RA 3,472,611 G→A G144G (GGC→GGT btuB ← vitamin B12/cobalamin outer membrane transporter
RA 3,497,165 G→A A65T (GCG→ACG)  fsaB → fructose‑6‑phosphate aldolase 2
RA 3,516,285 T→C intergenic (+20/‑47) hslU → / → menA molecular chaperone and ATPase component of HslUV protease/1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase
RA 3,532,412 T→C M236T (ATG→ACG)  cpxA → sensory histidine kinase in two‑component regulatory system with CpxR
RA 3,584,576 G→A P21S (CCA→TCA)  hemN ← coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent
RA 3,609,702 C→T E151E (GAG→GAA fre ← flavin reductase
RA 3,639,730 T→C K222R (AAA→AGA)  xerC ← site‑specific tyrosine recombinase
RA 3,657,262 C→T E273E (GAG→GAA wzyE ← predicted Wzy protein involved in ECA polysaccharide chain elongation
RA 3,704,876 C→T M164I (ATG→ATA trkD ← potassium transporter
RA 3,707,036 G→A A483T (GCC→ACC)  yieN → fused predicted transcriptional regulators
RA 3,727,747 T→C V168V (GTT→GTC pstA → phosphate transporter subunit
RA 3,735,429 G→A A323T (GCA→ACA)  bglH → carbohydrate‑specific outer membrane porin, cryptic
RA 3,739,327 C→T V195I (GTT→ATT)  yieH ← predicted hydrolase
MC JC 3,742,938 Δ1,336 bp insDinsC insD, insC
MC JC 3,746,913 Δ1,199 bp [tnaB][tnaB] [tnaB], insH, [tnaB]
MC JC 3,750,593 Δ1,199 bp insH insH
RA 3,806,473 G→A P198P (CCG→CCA yicI → predicted alpha‑glucosidase
RA 3,824,280 T→C pseudogene (413/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,326 T→C pseudogene (459/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,362 A→T pseudogene (495/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,368 G→A pseudogene (501/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,374 C→T pseudogene (507/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,380 2 bp→AA pseudogene (513‑514/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,410 T→A pseudogene (543/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,514 T→C pseudogene (647/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,524 T→G pseudogene (657/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,537 +G pseudogene (670/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,915,241 A→G E48G (GAG→GGG)  glyQ → glycine tRNA synthetase, alpha subunit
RA 3,980,009 T→C intergenic (‑6/+19) mdtF ← / ← mdtE multidrug transporter, RpoS‑dependent/multidrug resistance efflux transporter
RA 3,996,085 C→T A284V (GCC→GTC)  prlC → oligopeptidase A
RA 4,012,376 G→A A500T (GCC→ACC)  rbbA → fused ribosome‑associated ATPases
RA 4,017,703 T→C N1175D (AAC→GAC)  rhsB ← rhsB element core protein RshB
RA 4,032,019 C→T D650N (GAC→AAC)  zntA ← zinc, cobalt and lead efflux system
RA 4,036,723 G→A A184T (GCT→ACT)  ftsY → fused Signal Recognition Particle (SRP) receptor
RA 4,064,477 A→G E374E (GAA→GAG gntU → gluconate transporter, low affinity GNT 1 system
RA 4,083,312 G→A G325E (GGG→GAG)  rtcB → conserved hypothetical protein
RA 4,123,875 G→A R255H (CGC→CAC)  damX → hypothetical protein
MC JC 4,310,365 Δ1,199 bp insH[alsK] insH, [alsK]
RA 4,328,660 C→T V125I (GTC→ATC)  phnD ← phosphonate/organophosphate ester transporter subunit
RA 4,350,232 G→A R3C (CGC→TGC)  yjdF ← conserved inner membrane protein
RA 4,371,276 (A)5→3 intergenic (‑9/+296) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,392,090 G→T L655M (CTG→ATG)  yjeP ← predicted mechanosensitive channel
RA 4,407,692 C→T R325C (CGT→TGT)  hflK → modulator for HflB protease specific for phage lambda cII repressor
RA 4,443,410 G→A L179L (CTG→TTG)  ytfJ ← predicted transcriptional regulator
RA 4,477,260 G→A intergenic (‑45/+236) pyrL ← / ← yjgH pyrBI operon leader peptide/predicted mRNA endoribonuclease
RA 4,561,090 G→A intergenic (‑88/‑585) yjiC ← / → yjiD hypothetical protein/DNA replication/recombination/repair protein
RA 4,563,589 T→A K207M (AAG→ATG)  iadA ← isoaspartyl dipeptidase
RA 4,580,803 C→T pseudogene (1987/2721 nt) yjiV → ECK4334:JW5954:b4486; conserved hypothetical protein
RA 4,586,637 G→A P365L (CCG→CTG)  hsdM ← DNA methylase M
RA 4,588,402 C→T L1032L (CTG→CTA hsdR ← endonuclease R
RA 4,591,445 G→A T18I (ACA→ATA)  hsdR ← endonuclease R
RA 4,606,089 G→A N37N (AAC→AAT dnaT ← DNA biosynthesis protein
RA 4,643,405 T→C H182H (CAT→CAC creD → inner membrane protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 14522 14528 7 9 [0] [0] 5 dnaJ chaperone Hsp40, co‑chaperone with DnaK
* * ÷ W3110S.gb 15248 15253 6 9 [0] [0] 3 dnaJ chaperone Hsp40, co‑chaperone with DnaK
* * ÷ W3110S.gb 88491 88497 7 2 [0] [0] 8 fruR DNA‑binding transcriptional dual regulator
* * ÷ W3110S.gb 122325 122334 10 15 [1] [1] 4 pdhR DNA‑binding transcriptional dual regulator
* * ÷ W3110S.gb 159998 160000 3 2 [0] [0] 3 yadB glutamyl‑Q tRNA(Asp) synthetase
* * ÷ W3110S.gb 205159 205168 10 6 [0] [0] 4 dnaE DNA polymerase III alpha subunit
* * ÷ W3110S.gb 299130 299131 2 6 [1] [0] 6 yagR predicted oxidoreductase with molybdenum‑binding domain
* * ÷ W3110S.gb 371048 371077 30 10 [1] [0] 3 mhpC 2‑hydroxy‑6‑ketonona‑2,4‑dienedioic acid hydrolase
* * ÷ W3110S.gb 379244 379254 11 2 [0] [1] 8 frmR/yaiO regulator protein that represses frmRAB operon/hypothetical protein
* * ÷ W3110S.gb 422752 422775 24 7 [0] [0] 9 malZ maltodextrin glucosidase
* * ÷ W3110S.gb 464057 464074 18 2 [1] [1] 5 ybaW/ybaX conserved hypothetical protein/predicted aluminum resistance protein
* * ÷ W3110S.gb 482484 482506 23 19 [0] [0] 2 acrB multidrug efflux system protein
* * ÷ W3110S.gb 489762 489776 15 2 [1] [0] 2 priC primosomal replication protein N''
* * ÷ W3110S.gb 573868–575008 575999 992–2132 4 [0] [0] 146 insH–[nmpC] insH,[nmpC]
* * ÷ W3110S.gb 649172 649177 6 4 [0] [0] 24 citE citrate lyase, citryl‑ACP lyase (beta) subunit
* * ÷ W3110S.gb 672860 672872 13 2 [0] [0] 4 leuS leucyl‑tRNA synthetase
* * ÷ W3110S.gb 685594 685612 19 2 [1] [0] 2 gltL glutamate and aspartate transporter subunit
* * ÷ W3110S.gb 721512 721547 36 11 [0] [0] 6 kdpE DNA‑binding response regulator in two‑component regulatory system with KdpD
* * ÷ W3110S.gb 777547 777557 11 3 [0] [1] 2 tolA membrane anchored protein in TolA‑TolQ‑TolR complex
* * ÷ W3110S.gb 779950 779957 8 4 [0] [0] 5 pal peptidoglycan‑associated outer membrane lipoprotein
* * ÷ W3110S.gb 785656 785667 12 4 [1] [0] 9 ybgS conserved hypothetical protein
* * ÷ W3110S.gb 804648 804668 21 2 [0] [0] 4 ybhJ predicted hydratase
* * ÷ W3110S.gb 829012 829018 7 6 [1] [0] 4 ybhF fused predicted transporter subunits and ATP‑binding components of ABC superfamily
* * ÷ W3110S.gb 835105 835109 5 2 [0] [0] 13 dinG ATP‑dependent DNA helicase
* * ÷ W3110S.gb 907993 908007 15 8 [0] [0] 2 ybjT conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain
* * ÷ W3110S.gb 915614–915616 915647 32–34 9 [0] [0] 14 ybjE/aqpZ predicted transporter/aquaporin
* * ÷ W3110S.gb 964726 964744 19 6 [0] [0] 6 [ycaI] [ycaI]
* * ÷ W3110S.gb 1028972 1028978 7 9 [0] [0] 2 yccV DNA‑binding protein, hemimethylated
* * ÷ W3110S.gb 1117473 1117476 4 4 [0] [0] 24 lpxL lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase
* * ÷ W3110S.gb 1172355 1172362 8 6 [0] [1] 5 mfd transcription‑repair coupling factor
* * ÷ W3110S.gb 1192374 1192378 5 3 [0] [1] 6 purB adenylosuccinate lyase
* * ÷ W3110S.gb 1209512 1209517 6 2 [0] [0] 2 ycfK hypothetical protein
* * ÷ W3110S.gb 1227502 1227525 24 3 [1] [1] 2 minC cell division inhibitor
* * ÷ W3110S.gb 1263917 1263928 12 3 [0] [1] 6 ispE 4‑diphosphocytidyl‑2‑C‑methylerythritol kinase
* * ÷ W3110S.gb 1267740 1267742 3 14 [0] [0] 5 prmC N5‑glutamine methyltransferase, modifies release factors RF‑1 and RF‑2
* * ÷ W3110S.gb 1276090 1276100 11 5 [0] [0] 5 ychP predicted invasin
* * ÷ W3110S.gb 1292420 1292424 5 2 [1] [1] 3 rssB response regulator of RpoS
* * ÷ W3110S.gb 1313176 1313183 8 7 [0] [1] 4 tonB membrane spanning protein in TonB‑ExbB‑ExbD complex
* * ÷ W3110S.gb 1317658 1317675 18 4 [0] [1] 7 yciG hypothetical protein
* * ÷ W3110S.gb 1352917 1352926 10 4 [0] [0] 9 fabI/ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/conserved hypothetical protein
* * ÷ W3110S.gb 1359945 1359955 11 4 [1] [0] 3 puuP putrescine importer
* * ÷ W3110S.gb 1386111 1386119 9 4 [0] [0] 2 ycjX conserved hypothetical protein with nucleoside triphosphate hydrolase domain
* * ÷ W3110S.gb 1413582 1413582 1 3 [0] [0] 11 ydaO predicted C32 tRNA thiolase
* * ÷ W3110S.gb 1421315 1421330 16 6 [0] [0] 5 ydaG/racR hypothetical protein/predicted DNA‑binding transcriptional regulator
* * ÷ W3110S.gb 1431230 1431244 15 7 [0] [1] 2 stfR predicted tail fiber protein
* * ÷ W3110S.gb 1431621 1431625 5 9 [0] [1] 3 stfR predicted tail fiber protein
* * ÷ W3110S.gb 1463479 1463499 21 13 [1] [0] 8 paaJ predicted beta‑ketoadipyl CoA thiolase
* * ÷ W3110S.gb 1491877 1491880 4 4 [1] [0] 3 gapC
gapC
ECK1409:JW5906+JW1413:b4493; glyceraldehyde‑3‑phosphate dehydrogenase C
ECK1409:JW5906:b1417; glyceraldehyde‑3‑phosphate dehydrogenase C, C‑ter fragment
* * ÷ W3110S.gb 1513303 1513310 8 6 [0] [1] 2 ydcR/ydcS fused predicted DNA‑binding transcriptional regulator and predicted amino transferase/predicted spermidine/putrescine transporter subunit
* * ÷ W3110S.gb 1648467 1648492 26 2 [1] [1] 7 flxA/ydfW hypothetical protein/hypothetical protein
* * ÷ W3110S.gb 1672513 1672530 18 3 [1] [1] 4 ynfM predicted transporter
* * ÷ W3110S.gb 1732390 1732393 4 9 [1] [0] 7 lhr predicted ATP‑dependent helicase
* * ÷ W3110S.gb 1792034 1792034 1 7 [0] [0] 6 ydiU conserved hypothetical protein
* * ÷ W3110S.gb 1831086 1831096 11 2 [0] [1] 10 astD succinylglutamic semialdehyde dehydrogenase
* * ÷ W3110S.gb 1836788 1836796 9 4 [0] [0] 2 ydjZ conserved inner membrane protein
* * ÷ W3110S.gb 1853601 1853607 7 2 [0] [0] 3 pncA nicotinamidase/pyrazinamidase
* * ÷ W3110S.gb 1868233 1868240 8 6 [1] [0] 8 mipA/yeaG scaffolding protein for murein synthesizing machinery/conserved hypothetical protein with nucleoside triphosphate hydrolase domain
* * ÷ W3110S.gb 2041768 2041795 28 12 [0] [0] 2 yedY predicted reductase
* * ÷ W3110S.gb 2079303 2079309 7 12 [0] [0] 4 yeeU antitoxin of the YeeV‑YeeU toxin‑antitoxin system
* * ÷ W3110S.gb 2186663 2186693 31 4 [1] [1] 6 yegX predicted hydrolase
* * ÷ W3110S.gb 2202199 2202201 3 8 [0] [0] 11 molR
molR
DNA‑binding transcriptional regulator; ECK2108:JW2102+JW5915+JW5916:b4499
DNA‑binding transcriptional regulator, middle fragment; ECK2108:JW5915:b2116
* * ÷ W3110S.gb 2210260 2210261 2 5 [0] [0] 6 yehM hypothetical protein
* * ÷ W3110S.gb 2232522 2232570–2232543 22–49 5 [0] [1] 2 yohH/dusC conserved hypothetical protein/tRNA‑dihydrouridine synthase C
* * ÷ W3110S.gb 2355268 2355299 32 4 [1] [0] 4 glpQ periplasmic glycerophosphodiester phosphodiesterase
* * ÷ W3110S.gb 2403200 2403214 15 5 [0] [1] 5 nuoG NADH:ubiquinone oxidoreductase, chain G
* * ÷ W3110S.gb 2472080 2472083 4 7 [0] [0] 3 intS predicted prophage CPS‑53 integrase
* * ÷ W3110S.gb 2498099 2498099 1 13 [0] [0] 7 frc formyl‑CoA transferase, NAD(P)‑binding
* * ÷ W3110S.gb 2569115 2569147 33 5 [1] [0] 3 eutE predicted aldehyde dehydrogenase, ethanolamine utilization protein
* * ÷ W3110S.gb 2647056 2647062 7 2 [0] [0] 3 yfhM conserved hypothetical protein
* * ÷ W3110S.gb 2749325 2749332 8 3 [0] [0] 7 grpE heat shock protein
* * ÷ W3110S.gb 2803992 2803997 6 5 [0] [0] 2 proV glycine betaine transporter subunit
* * ÷ W3110S.gb 2827387 2827403 17 3 [1] [1] 2 gutM DNA‑binding transcriptional activator
* * ÷ W3110S.gb 2940001 2940001 1 3 [0] [0] 5 ygdD conserved inner membrane protein
* * ÷ W3110S.gb 3007124 3007132 9 7 [1] [0] 3 ygeX 2,3‑diaminopropionate ammonia‑lyase
* * ÷ W3110S.gb 3010354 3010357 4 4 [1] [0] 5 yqeA predicted amino acid kinase
* * ÷ W3110S.gb 3315157 3315182 26 3 [0] [1] 2 infB fused protein chain initiation factor 2, IF2
* * ÷ W3110S.gb 3438714 3438721 8 4 [0] [0] 17 hemE uroporphyrinogen decarboxylase
* * ÷ W3110S.gb 3759296 3759304 9 4 [0] [0] 3 recF gap repair protein
* * ÷ W3110S.gb 3859257 3859260 4 3 [0] [0] 2 yibK/lldD predicted rRNA methylase/L‑lactate dehydrogenase, FMN‑linked
* * ÷ W3110S.gb 3875061 3875069–3875062 2–9 4 [0] [0] 7 rhsA rhsA element core protein RshA
* * ÷ W3110S.gb 4083703 4083711 9 4 [0] [0] 2 rtcA RNA 3'‑terminal phosphate cyclase
* * ÷ W3110S.gb 4120616 4120626 11 2 [1] [0] 4 hofQ predicted fimbrial transporter
* * ÷ W3110S.gb 4521187 4521192 6 3 [0] [0] 3 fecA ferric citrate outer membrane transporter
* * ÷ W3110S.gb 4554534 4554538 5 2 [0] [0] 7 fimH/gntP minor component of type 1 fimbriae/fructuronate transporter

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 274373NA (NA)146 (3.040) 9/110 0.3 100% intergenic (‑32/‑176) insH/mmuP IS5 transposase and trans‑activator/predicted S‑methylmethionine transporter
?W3110S.gb 576000 = 0 (0.000)pseudogene (109/1128 nt) nmpC DLP12 prophage region; ECK0544:JW5078:b0553; truncated outer membrane porin
* ? W3110S.gb 314453 =45 (0.940)26 (0.570)
+TCA
7/104 0.4 55.0% coding (873/888 nt) eaeH attaching and effacing protein, pathogenesis factor
?W3110S.gb = 3920281 0 (0.000)coding (88/213 nt) cspA major cold shock protein
* ? W3110S.gb 314453 =45 (0.940)109 (2.360)
+TC
17/106 0.0 81.6% coding (873/888 nt) eaeH attaching and effacing protein, pathogenesis factor
?W3110S.gb 3932930 = 6 (0.120)coding (219/1608 nt) dppA dipeptide transporter
* ? W3110S.gb = 315707NA (NA)46 (0.960) 8/110 0.4 100% coding (654/687 nt) ykgA predicted DNA‑binding transcriptional regulator
?W3110S.gb 576911 = 0 (0.000)coding (76/498 nt) ybcS predicted lysozyme
* ? W3110S.gb 566000 =NA (NA)43 (0.900) 10/110 0.3 100% intergenic (+90/‑56) ybcD/insE DLP12 prophage region; ECK0532:JW0527:b4508; predicted replication protein fragment/IS3 element protein InsE
?W3110S.gb = 576913 0 (0.000)coding (78/498 nt) ybcS predicted lysozyme
* ? W3110S.gb 1209367 =5 (0.100)40 (1.180) 7/78 0.3 85.5% coding (290/630 nt) ycfK hypothetical protein
?W3110S.gb 1211196 = 10 (0.300)pseudogene (37/537 nt) stfE ECK1143:JW5172:b1157; e14 prophage region; predicted side tail fiber protein fragment
* ? W3110S.gb = 12093825 (0.100)30 (0.890) 6/78 0.3 81.6% coding (305/630 nt) ycfK hypothetical protein
?W3110S.gb = 1211179 10 (0.300)pseudogene (54/537 nt) stfE ECK1143:JW5172:b1157; e14 prophage region; predicted side tail fiber protein fragment
* ? W3110S.gb 2018571 =7 (0.150)39 (0.860) 10/104 0.2 85.5% coding (567/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb 2519723 = NA (NA)intergenic (+33/‑54) nupC/insL nucleoside (except guanosine) transporter/predicted transposase
* ? W3110S.gb = 201858311 (0.230)52 (1.120) 7/106 0.5 83.1% coding (579/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb = 2521059 NA (NA)intergenic (+170/+30) insL/yfeA predicted transposase/predicted diguanylate cyclase
* ? W3110S.gb = 2173564NA (NA)52 (1.100) 12/108 0.2 88.3% pseudogene (307/2041 nt)
pseudogene (307/339 nt)
gatR
gatR
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
DNA‑binding transcriptional regulator, C‑ter fragment; ECK2083:JW2074:b2090
?W3110S.gb 3920278 = 7 (0.150)coding (91/213 nt) cspA major cold shock protein
* ? W3110S.gb = 2412225NA (NA)55 (1.150) 13/110 0.1 77.5% intergenic (‑55/‑782) insA/yfbQ IS1 element protein/predicted aminotransferase
?W3110S.gb = 3748807 16 (0.330)coding (176/984 nt) tnaB tryptophan transporter of low affinity
* ? W3110S.gb 3748799 =15 (0.310)79 (1.650) 13/110 0.1 84.0% coding (184/984 nt) tnaB tryptophan transporter of low affinity
?W3110S.gb 4056222 = NA (NA)intergenic (‑564/+15) yrhB/insB hypothetical protein/IS1 transposase InsAB'
* ? W3110S.gb = 454737216 (0.330)26 (0.650) 6/92 0.4 75.0% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?W3110S.gb = 4547668 4 (0.100)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin