Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R2
|
489 |
0.0 |
1652083 |
58.3% |
963164 |
55.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,314,275 |
C→G |
T110T (ACG→ACC) |
yciB ← |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,314,275 | 0 | C | G | 99.6%
| 655.6
/ NA
| 227 | T110T (ACG→ACC) | yciB | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (0/226); minor base T (0/1); total (0/227) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 227 total aligned reads displayed.
TTCAGCTTCGACCATACCGGTTGCGGCAGCGTGAGTTCTTTACCCAGCATCCGCTGAATTAG > W3110S.gb/1314246‑1314307
|
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:464269/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:632403/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:624755/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:601384/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:58376/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:567708/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:547037/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:524009/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:5157/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:502029/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:490611/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:478976/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:648760/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:456990/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:435911/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:424170/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:42291/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:397584/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:3733/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:353189/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:3224/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:3115/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1006173/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:27399/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:813885/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:982526/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:96851/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:954463/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:93568/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:929558/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:91032/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:883995/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:875678/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:862198/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:843310/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:827440/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:290227/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:805390/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:785690/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:780842/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:777516/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:755446/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:752193/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:70917/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:70555/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:685676/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:671624/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:666332/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1290326/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1405560/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:249781/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1380406/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1367176/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1363201/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1350165/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1334300/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1327289/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1318716/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1311836/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1302226/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1391715/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:127772/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1237399/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1222528/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1216456/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1179385/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1138319/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1110448/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1088214/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1048613/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1034199/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1027535/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1572994/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:246092/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:243260/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:234991/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:220115/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:210873/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:195738/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:164747/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1624787/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1616619/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1605686/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:155723/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1542922/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1529275/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1488935/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1481567/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1469983/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1452614/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1450784/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1444608/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:1440368/62‑1 (MQ=255)
ttCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCACCATCCGCTGAATTAg < 1:1053420/62‑1 (MQ=255)
ttCAGCTTCGAACATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:637754/62‑1 (MQ=255)
tCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:739737/61‑1 (MQ=255)
tCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:898452/61‑1 (MQ=255)
tCAGCTTCGACCATACCGATTGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:590924/61‑1 (MQ=255)
attGCGGCAGGGTGAGTTCTTTACCCAGCATCCGCTGAATTAg < 1:127243/42‑1 (MQ=255)
tGCGGCAGGGTGAGTTCTTTTCCCAGCATCCGCTGAATTAg < 1:1425543/41‑1 (MQ=38)
|
TTCAGCTTCGACCATACCGGTTGCGGCAGCGTGAGTTCTTTACCCAGCATCCGCTGAATTAG > W3110S.gb/1314246‑1314307
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
