Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,484,463 T→C I38V (ATC→GTC)  azoR ← NADH‑azoreductase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,484,4630TC100.0% 329.5 / NA 113I38V (ATC→GTC) azoRNADH‑azoreductase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (113/0);  total (113/0)

Only 100 of 113 total aligned reads displayed.

CGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGT  >  W3110S.gb/1484434‑1484494
                             |                               
cGGATTTGCAGCCAGGTCGCGAACGGTGACTtcat                            >  1:131255/1‑35 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCtttt             >  1:448320/1‑50 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCt                >  1:492833/1‑47 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTc            >  1:717304/1‑51 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGcc       >  1:859058/1‑56 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTg   >  1:100198/1‑60 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTg   >  1:1473877/1‑60 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:35819/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:642188/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:592675/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:587445/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:287087/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:574070/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:532875/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:50535/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:492896/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:294347/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:39662/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:458027/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:363214/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:441597/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:439167/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:395359/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:428768/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:427207/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:422872/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:411804/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:833856/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:991086/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:985678/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:978673/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:970797/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:960899/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:957735/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:952653/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:900528/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:886954/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:874232/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:864917/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:632216/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:831258/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:829549/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:814915/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:814532/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:789106/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:783789/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:783219/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:770607/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:761208/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:683861/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:280568/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1194666/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1291331/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1271578/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:125674/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1251503/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1230555/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1224754/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1223485/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1223170/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:122224/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1198602/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1196667/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1196477/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:252124/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1191939/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1162425/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1146941/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1137069/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1105014/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1097059/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1073116/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1066831/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1051182/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1035195/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1006929/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1566785/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:284925/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:25134/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:217899/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:208291/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:195123/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:187066/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:171075/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:165510/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1639273/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1580182/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1546518/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1520363/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1499902/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1471962/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:145333/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1445708/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1352581/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1340891/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1330381/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:1315496/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:129700/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATAAGCGGAGTGCTTTTCGCGCCATTGt  >  1:652889/1‑61 (MQ=255)
cGGATTTGCAGCCAGGTCGCGAACGGTGACTTAATCAGCGGAGTGCTTTTCGCGCCATTGt  >  1:178842/1‑61 (MQ=255)
                             |                               
CGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGT  >  W3110S.gb/1484434‑1484494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A