Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,484,463 |
T→C |
17.6% |
I38V (ATC→GTC) |
azoR ← |
NADH‑azoreductase, FMN‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,484,463 | 0 | T | C | 17.6%
| 155.9
/ 28.5
| 68 | I38V (ATC→GTC) | azoR | NADH‑azoreductase, FMN‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (24/32); new base C (7/5); total (31/37) |
| Fisher's exact test for biased strand distribution p-value = 3.58e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01 |
CGCAGAGCGCCAACCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTGAGAG > W3110S.gb/1484396‑1484530
|
cGCAGAGCGCCAACCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGACTtc < 1:497391/71‑1 (MQ=255)
agagCGCCAACCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTtcatc < 1:1571332/71‑1 (MQ=255)
gcgcCAACCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGc > 1:2130016/1‑71 (MQ=255)
cgcCAACCAGTTCGCCATCCAGTATCGGAATCGGATTTGCAGCCAGGGCGCGAACGGTGATTtca < 1:1209987/65‑1 (MQ=255)
aaCCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGt > 1:3813012/1‑71 (MQ=255)
aCCAGTACGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTg > 1:659276/1‑71 (MQ=255)
aGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTCCATCAGCGGAg > 1:3670702/1‑66 (MQ=255)
cGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGTAGTGCTTTTcg > 1:1206264/1‑71 (MQ=255)
cATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGATTGCTTTTCgcgc < 1:3076178/71‑1 (MQ=255)
cATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCgcgc < 1:4537910/71‑1 (MQ=255)
aTCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCgcgc > 1:2531844/1‑70 (MQ=255)
aTCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCgcgc > 1:4666710/1‑70 (MQ=255)
aTCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGcc < 1:4553904/71‑1 (MQ=255)
tCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTtc < 1:1298128/47‑1 (MQ=255)
tCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTtc < 1:5050564/47‑1 (MQ=255)
tCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTtc < 1:1081587/47‑1 (MQ=255)
tCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTtc < 1:4500614/47‑1 (MQ=255)
tCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCg < 1:4152135/54‑1 (MQ=255)
cAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCAt < 1:3623675/70‑1 (MQ=255)
cAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCAt < 1:4920643/70‑1 (MQ=255)
aGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCAt < 1:3639173/69‑1 (MQ=255)
gTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTg > 1:1814586/1‑70 (MQ=255)
ccGGAATCGGATTTGCATCCAGGTCGCGAACGGTGATTTCATCAGCTGAGTGCTTTTCGCGCCATTGTTca < 1:2080547/71‑1 (MQ=255)
ggAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAg > 1:937290/1‑48 (MQ=255)
aaTCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTcaacaa < 1:2374575/71‑1 (MQ=255)
ttCGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt < 1:3524716/62‑1 (MQ=255)
aTCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTcaacaa > 1:1669568/1‑70 (MQ=255)
aTCGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt < 1:5034998/63‑1 (MQ=255)
aTCGGATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGt < 1:4233563/63‑1 (MQ=255)
tCGGATTTGCAGCCAGGTCGCGAACGGTGATTtca < 1:4430830/35‑1 (MQ=255)
tCGGATTTGCAGCCAGGTCGCGAACGGTGATTtca < 1:3986063/35‑1 (MQ=255)
tCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTc < 1:4454317/52‑1 (MQ=255)
tCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTc < 1:4313505/52‑1 (MQ=255)
tCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACaaa > 1:892011/1‑70 (MQ=255)
ggATTTGCAGCCAGGTCGCGAACGGTGATTtcatc > 1:2250317/1‑35 (MQ=255)
ggATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTcaacaa > 1:3339518/1‑67 (MQ=255)
ggATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAaata < 1:4306187/71‑1 (MQ=255)
ggATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAg > 1:4426774/1‑42 (MQ=255)
ggATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAg > 1:514466/1‑42 (MQ=255)
ggATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAg > 1:3335535/1‑42 (MQ=255)
ggATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAg > 1:3951733/1‑42 (MQ=255)
ggATTTGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAg > 1:2468196/1‑42 (MQ=255)
aTTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAaataat > 1:5083749/1‑71 (MQ=255)
aTTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAaataa < 1:2199057/70‑1 (MQ=255)
ttGCAGCCAGGTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCg > 1:1200951/1‑71 (MQ=255)
cAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGAc < 1:2809369/71‑1 (MQ=255)
cAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGAc < 1:454979/71‑1 (MQ=255)
cAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACt < 1:3835836/71‑1 (MQ=255)
ggTCGCGAACGGTGACTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGa > 1:2157265/1‑71 (MQ=255)
gTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTcaacaa < 1:3270872/53‑1 (MQ=255)
gTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTc < 1:4413076/48‑1 (MQ=255)
gTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTc < 1:3645720/48‑1 (MQ=255)
cgcgAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACaaaa > 1:2174963/1‑53 (MQ=255)
cgcgAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGAt > 1:1835376/1‑69 (MQ=255)
gcgAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGAt > 1:4135979/1‑68 (MQ=255)
gcgAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGAt > 1:4622991/1‑68 (MQ=255)
gcgAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTa < 1:2504296/70‑1 (MQ=255)
cgAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGa < 1:3567450/71‑1 (MQ=255)
gAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTcaacaa < 1:2456360/48‑1 (MQ=255)
cGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAaata < 1:5138676/49‑1 (MQ=255)
cGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTga < 1:4066656/71‑1 (MQ=255)
ggTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACt > 1:1922376/1‑68 (MQ=255)
gTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACaa > 1:765457/1‑56 (MQ=255)
gTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGAt > 1:4907910/1‑61 (MQ=255)
tGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTa < 1:4657932/62‑1 (MQ=255)
gATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTGagag > 1:3467090/1‑70 (MQ=255)
gATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTGagag > 1:3461508/1‑70 (MQ=255)
gATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTGagag > 1:3346875/1‑70 (MQ=255)
|
CGCAGAGCGCCAACCAGTTCGCCATCCAGTACCGGAATCGGATTTGCAGCCAGGTCGCGAACGGTGATTTCATCAGCGGAGTGCTTTTCGCGCCATTGTTCAACAAAATAATCGGACAACTGATTAGACTGAGAG > W3110S.gb/1484396‑1484530
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
