Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,495,788 C→A R535R (CGC→CGA)  trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,495,7880CA100.0% 643.0 / NA 222R535R (CGC→CGA) trgmethyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/222);  total (0/222)

Only 100 of 222 total aligned reads displayed.

ACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAA  >  W3110S.gb/1495764‑1495825
                        |                                     
aCATTCTGTGTCGGCAGAACCACGAGGAGCTTGTGAACCAGTTATTTTTGCTACTGTGTGaa  <  1:1621578/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:268882/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:504785/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:456213/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:445007/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:428974/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:41263/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:354624/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:343363/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:337406/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:30965/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:28908/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:517228/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:262313/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:241051/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:233452/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1626257/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1613085/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1602826/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1592574/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1584900/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:675120/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:969559/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:909214/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:830471/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:798726/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:794485/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:783158/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:756477/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:746378/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:701164/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:212200/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:662720/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:660691/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:619805/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:605253/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:585989/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:567710/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:554175/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:552946/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:539639/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1565977/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1233643/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1243939/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1260837/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:120486/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1284927/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1297810/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1332508/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1113865/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1366180/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1112369/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1418787/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1106404/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1426496/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1434509/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1529582/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1055968/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1033708/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1024541/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1491325/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1451703/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1026424/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTCaa  <  1:580709/62‑1 (MQ=255)
aCATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGGGaa  <  1:630468/62‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1150346/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:155011/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1221275/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:93864/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:916419/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1180646/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1132662/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:866121/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:704710/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:804385/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1065391/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:110061/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:777444/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1271548/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1608841/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1514228/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1492616/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:168246/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1008462/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:22121/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:147909/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1453617/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:249300/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1432484/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:332963/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:140766/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:385477/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1345444/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:417656/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1310613/61‑1 (MQ=255)
 cATTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1248572/61‑1 (MQ=255)
  aTTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:484934/60‑1 (MQ=255)
  aTTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:487903/60‑1 (MQ=255)
  aTTCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:1516813/60‑1 (MQ=255)
   ttCTGTGTCGGCAGAACCACGAGGAGCTGGTGAACCAGTTAGTTTTGCTACGGTGTGaa  <  1:116319/59‑1 (MQ=255)
                        |                                     
ACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAA  >  W3110S.gb/1495764‑1495825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A