Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,584,624 A→G intergenic (‑386/+16) ydeN ← / ← ydeO conserved hypothetical protein/predicted DNA‑binding transcriptional acfivator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,584,6240AG100.0% 361.5 / NA 124intergenic (‑386/+16)ydeN/ydeOconserved hypothetical protein/predicted DNA‑binding transcriptional acfivator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/124);  total (0/124)

Only 100 of 124 total aligned reads displayed.

TGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAC  >  W3110S.gb/1584619‑1584680
     |                                                        
tGACTGCTCGTTATCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1582727/62‑1 (MQ=255)
tGACTGCTCGTTAGCATATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCAttc  <  1:147429/62‑3 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTGAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:854219/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:44408/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:594185/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:590513/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:572533/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:551655/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:537703/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:531873/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:486469/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:473110/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:466454/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:459941/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:605755/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:443510/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:442963/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:395821/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:395375/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:381227/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:361736/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:352908/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:33481/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:321126/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1011947/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:841904/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:988816/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:979619/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:968761/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:964366/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:945815/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:923707/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:899563/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:862446/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:857296/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:856910/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:843032/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:603820/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:835539/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:818867/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:800576/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:79556/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:777783/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:734850/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:70666/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:655685/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:63447/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:627178/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:318759/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1208107/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:30236/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1389600/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1317309/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1312807/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1302571/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1285913/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1281809/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1281232/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1255805/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1221813/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1220249/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:121856/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1395432/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1183664/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1168412/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1158195/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:112863/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1122289/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1107803/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1093001/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1042701/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1026625/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1024092/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1013263/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1610456/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:295548/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:277541/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:275131/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:252882/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:246843/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:242593/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:213403/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:181023/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1410846/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1607471/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1559333/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1538475/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1502010/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1489876/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1479282/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1456976/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1456624/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1436603/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAATCAAATAGATAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:953098/62‑1 (MQ=255)
tGACTGCTCGTTAGCAAATAACCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1559608/62‑1 (MQ=255)
 gACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:428198/61‑1 (MQ=255)
 gACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:182777/61‑1 (MQ=255)
 gACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:750475/61‑1 (MQ=255)
 gACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:331489/61‑1 (MQ=255)
 gACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAc  <  1:1422402/61‑1 (MQ=255)
     |                                                        
TGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAC  >  W3110S.gb/1584619‑1584680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A