Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,584,624 |
A→G |
intergenic (‑386/+16) |
ydeN ← / ← ydeO |
conserved hypothetical protein/predicted DNA‑binding transcriptional acfivator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,584,624 | 0 | A | G | 100.0%
| 289.2
/ NA
| 77 | intergenic (‑386/+16) | ydeN/ydeO | conserved hypothetical protein/predicted DNA‑binding transcriptional acfivator |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/48); total (29/48) |
GCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACC > W3110S.gb/1584574‑1584681
|
gCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:2661824/62‑1 (MQ=255)
gCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:4360793/62‑1 (MQ=255)
gCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:4328578/62‑1 (MQ=255)
gggAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAaata < 1:480901/62‑1 (MQ=255)
gggAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAaata < 1:4626081/62‑1 (MQ=255)
gCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAg > 1:4102225/1‑61 (MQ=255)
gCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAg > 1:4379596/1‑61 (MQ=255)
gCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAg > 1:1491263/1‑61 (MQ=255)
gCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAg > 1:1496802/1‑61 (MQ=255)
gCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGc < 1:1974726/62‑1 (MQ=255)
gCCCCATTTAATTCTTACGCAGAGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAg > 1:4242304/1‑61 (MQ=255)
ccccATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGc < 1:273207/61‑1 (MQ=255)
cccATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGc < 1:843245/60‑1 (MQ=255)
cATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTaa > 1:4356778/1‑61 (MQ=255)
cATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTaa > 1:1946039/1‑61 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:3205353/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:3969676/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:3123254/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:1091077/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:2965974/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:2108906/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:4291017/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:4304093/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:1863191/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:1723175/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:1687880/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:1343280/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:1239759/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:119540/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:777203/41‑1 (MQ=255)
tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCAt < 1:2602030/62‑1 (MQ=255)
cTTACGGAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:4199797/36‑1 (MQ=255)
cTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:3485884/36‑1 (MQ=255)
cTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:3048983/36‑1 (MQ=255)
cTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:2562664/36‑1 (MQ=255)
cTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa < 1:1859306/36‑1 (MQ=255)
cTTACGCAGCGTGTGTGGTTGACTGCGCGTTAGCaa < 1:3440666/36‑1 (MQ=38)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCa > 1:2997097/1‑41 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:2255960/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:1129668/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:819255/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:520707/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:517845/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:1295167/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:4444678/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:1344317/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:1648356/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:2114825/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:2957530/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:3730189/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:2916960/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:3481922/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:3038542/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:3476344/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:2406376/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt > 1:3460523/1‑62 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCg < 1:3300265/61‑1 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCg < 1:3094420/61‑1 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCg < 1:133763/61‑1 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCg < 1:4615061/61‑1 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCg < 1:2417244/61‑1 (MQ=255)
tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATCCATCgt > 1:237197/1‑62 (MQ=255)
gCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg < 1:1888704/60‑1 (MQ=255)
gCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg < 1:1168401/60‑1 (MQ=255)
gCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg < 1:836920/60‑1 (MQ=255)
gCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg < 1:3493107/60‑1 (MQ=255)
cAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg < 1:4575332/59‑1 (MQ=255)
cAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg < 1:4129560/59‑1 (MQ=255)
gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg < 1:2007785/62‑1 (MQ=255)
gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg < 1:3474509/62‑1 (MQ=255)
gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg < 1:3149258/62‑1 (MQ=255)
gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg < 1:2450691/62‑1 (MQ=255)
gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg < 1:306829/62‑1 (MQ=255)
gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg < 1:622272/62‑1 (MQ=255)
gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg < 1:3789974/62‑1 (MQ=255)
gACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAcc > 1:1677416/1‑62 (MQ=255)
gACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAcc > 1:2209589/1‑62 (MQ=255)
|
GCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACC > W3110S.gb/1584574‑1584681
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
