Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,699,047 C→G A236A (GCG→GCC)  hdhA ← 7alpha‑hydroxysteroid dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,699,0470CG97.7% 365.3 / ‑2.5 128A236A (GCG→GCC) hdhA7alpha‑hydroxysteroid dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base G (125/0);  total (128/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCG  >  W3110S.gb/1698995‑1699072
                                                    |                         
gttCCTGTCCCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:478127/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGGATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:544643/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTCCCCAGCTGGctgcag                    >  1:863863/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGgctgca                     >  1:601267/3‑59 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGgctgca                     >  1:1599571/3‑59 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGgctgca                     >  1:1617501/3‑59 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGgctgca                     >  1:1341493/3‑59 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:485587/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:59119/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:575131/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:566095/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:555901/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:549780/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:533416/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:527708/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:520995/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:284917/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:469877/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:466543/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:434311/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:420747/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:369564/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:339387/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:336049/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:331176/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:325200/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:298138/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:262543/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:76133/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:951141/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:913936/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:882132/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:859806/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:853540/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:850741/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:823715/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:79992/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:791573/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:773136/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:770971/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:596540/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:746604/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:739705/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:712718/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:706613/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:67342/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:667114/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:658644/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:657737/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:631346/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:629846/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:60619/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1267703/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1438728/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1432476/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1428980/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1422308/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1413759/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1405534/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1399770/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1385479/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1363769/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1281795/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1279266/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1461673/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1258023/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1183453/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1178154/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1174750/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1132964/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1131231/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1125285/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1103491/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1065157/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1049954/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1019245/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1622445/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:100243/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:245982/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:244402/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:23037/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:222634/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:221274/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:219379/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:204791/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:180141/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:178482/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:175751/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:264760/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1601096/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1593984/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1587786/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1586601/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1581577/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1580359/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:156438/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1519732/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1488665/3‑60 (MQ=255)
gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag                    >  1:1482254/3‑60 (MQ=255)
                accGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgc   >  1:1577707/1‑61 (MQ=255)
                                                    |                         
GCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCG  >  W3110S.gb/1698995‑1699072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A