Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,761,548 G→A P230P (CCC→CCT)  sufS ← selenocysteine lyase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,761,5480GA100.0% 296.5 / NA 104P230P (CCC→CCT) sufSselenocysteine lyase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (104/0);  total (104/0)

Only 100 of 116 total aligned reads displayed.

CCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGC  >  W3110S.gb/1761491‑1761552
                                                         |    
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGGCAATTCCGGTAGGGc  >  1:1194025/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCTATTCCGGTaggg   >  1:234824/1‑61 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCTGGTAGGGc  >  1:706244/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTaggg   >  1:394844/1‑61 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTaggg   >  1:1520638/1‑61 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTaggg   >  1:56415/1‑61 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTaggg   >  1:1268270/1‑61 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTaggg   >  1:244003/1‑61 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTaggg   >  1:912985/1‑61 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:568450/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:632214/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:667451/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:614260/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:394881/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:603868/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:598540/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:585323/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:585131/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:584503/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:477205/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:3999/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:51957/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:513660/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:420799/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:449643/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:50406/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:489899/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:482002/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:818523/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:9829/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:972556/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:966978/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:953547/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:919441/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:910921/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:907609/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:889472/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:887743/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:836193/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:82624/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:638558/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:814772/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:813803/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:803118/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:772736/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:770787/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:756691/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:744002/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:714528/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:388987/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:641925/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1278360/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1016887/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1458568/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1446271/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:143866/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1416074/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1400524/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1398288/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1350822/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1311909/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1300816/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1288236/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1498917/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1242968/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1238679/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1238341/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1232321/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:118883/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1134236/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1101232/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1082700/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1056271/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1055808/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1036751/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:230349/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:375239/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:364989/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:337378/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:310722/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:300315/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:289645/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:253743/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:230968/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1523842/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:219899/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:180560/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1638024/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1616912/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1616606/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1582687/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1532266/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1530637/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1527357/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:1527040/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTCAGGTaggg   >  1:893550/1‑61 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTACGGTaggg   >  1:942084/1‑61 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAATTACGGTAGGGc  >  1:408893/1‑62 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTGACATAAAGAATGCCAAGTCCGGTaggg   >  1:236782/1‑61 (MQ=255)
ccACGGCGGCATCTCCTGCAACAAGGATTCTTTGACATAAAGAATGCCAATTCCGGTAGGGc  >  1:617522/1‑62 (MQ=255)
                                                         |    
CCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGC  >  W3110S.gb/1761491‑1761552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A