Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R2
|
489 |
0.0 |
1652083 |
58.3% |
963164 |
55.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,016,320 |
T→G |
D535E (GAT→GAG) |
ygfK → |
predicted oxidoreductase, Fe‑S subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,016,320 | 0 | T | G | 100.0%
| 17.3
/ NA
| 8 | D535E (GAT→GAG) | ygfK | predicted oxidoreductase, Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (8/0); total (8/0) |
CTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACG > W3110S.gb/3016273‑3016334
|
cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg > 1:1080570/1‑62 (MQ=255)
cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg > 1:1199429/1‑62 (MQ=255)
cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg > 1:1544573/1‑62 (MQ=255)
cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg > 1:1548252/1‑62 (MQ=255)
cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg > 1:1628081/1‑62 (MQ=255)
cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg > 1:770962/1‑62 (MQ=255)
cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg > 1:808602/1‑62 (MQ=255)
cTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGAGGAATATAAGCAACg > 1:910444/1‑62 (MQ=255)
|
CTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACG > W3110S.gb/3016273‑3016334
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
