Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R2
|
489 |
0.0 |
1652083 |
58.3% |
963164 |
55.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,318,163 |
C→A |
intergenic (‑19/‑329) |
metY ← / → argG |
tRNA‑Met/argininosuccinate synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,318,163 | 0 | C | A | 100.0%
| 25.3
/ NA
| 11 | intergenic (‑19/‑329) | metY/argG | tRNA‑Met/argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/11); total (0/11) |
GCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAA > W3110S.gb/3318121‑3318182
|
gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa < 1:1035025/62‑1 (MQ=255)
gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa < 1:1096986/62‑1 (MQ=255)
gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa < 1:115179/62‑1 (MQ=255)
gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa < 1:1266975/62‑1 (MQ=255)
gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa < 1:1281858/62‑1 (MQ=255)
gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa < 1:133736/62‑1 (MQ=255)
gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa < 1:1379308/62‑1 (MQ=255)
gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa < 1:197770/62‑1 (MQ=255)
gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa < 1:239882/62‑1 (MQ=255)
gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa < 1:380538/62‑1 (MQ=255)
gCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTATACTCGTTTTGGGTaaaaa < 1:396270/62‑1 (MQ=255)
|
GCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAA > W3110S.gb/3318121‑3318182
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
