Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R2
|
489 |
0.0 |
1652083 |
58.3% |
963164 |
55.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,421,562 |
2 bp→TA |
coding (383‑384/1104 nt) |
yhdY → |
predicted amino‑acid transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,421,562 | 0 | C | T | 100.0%
| 54.1
/ NA
| 21 | A128V (GCC→GTA) | yhdY | predicted amino‑acid transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/21); total (0/21) |
| * | W3110S.gb | 3,421,563 | 0 | C | A | 95.2%
| 52.3
/ ‑5.2
| 21 | A128V (GCC→GTA) | yhdY | predicted amino‑acid transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (0/20); total (0/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTCTGGAAAATACTCCCGCATCGCGGTCGCTATATTGCGGCCTGGGCGGTGATTTACCCACT > W3110S.gb/3421522‑3421583
||
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTCTGGGCGGTGATTTACCCACt < 1:1374730/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCTGTGATTTACCCACt < 1:362455/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:155262/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:943175/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:770518/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:723570/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:639719/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:490062/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:212276/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:200795/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1572093/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1052310/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1551901/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1506106/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1484456/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1350224/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1268323/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1210871/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1155088/62‑1 (MQ=255)
ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1085912/62‑1 (MQ=255)
ttCTCGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:179376/62‑1 (MQ=255)
||
TTCTGGAAAATACTCCCGCATCGCGGTCGCTATATTGCGGCCTGGGCGGTGATTTACCCACT > W3110S.gb/3421522‑3421583
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
