Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,643,606 A→G C641C (TGT→TGC)  cyaA ← adenylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,643,6060AG100.0% 373.5 / NA 127C641C (TGT→TGC) cyaAadenylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (127/0);  total (127/0)

Only 100 of 127 total aligned reads displayed.

AGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCTATAACAGAAGACTTC  >  W3110S.gb/3643559‑3643617
                                               |           
aGTTGCTGCACGCGAGTACGGATTAAGCTGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1433889/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGCTGCTGGCTGTAGCAGAAGACTTc  >  1:911652/1‑59 (MQ=38)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:390899/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:57171/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:56569/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:560975/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:553779/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:510966/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:503696/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:500941/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:495143/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:462659/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:457597/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:457159/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:427840/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:613007/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:390474/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:386525/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:375880/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:373059/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:365193/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:357174/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:3307/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:312219/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:289139/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:279773/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:226240/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:775963/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:9938/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:992949/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:987296/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:938523/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:917255/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:911377/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:896482/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:868476/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:86320/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:860933/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:795026/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:226005/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:770426/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:726163/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:693838/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:683258/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:681920/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:65384/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:64833/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:647596/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:644526/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:630334/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:617614/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1198532/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1358691/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1335585/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1318687/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:131425/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1299528/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1298433/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1268326/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1260758/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1244650/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1233071/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1221057/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1219637/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1382903/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1189218/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:117721/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1163222/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1162572/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1155555/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1148140/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1103762/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1101086/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1057936/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1037575/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1018922/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1576679/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:225039/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:210019/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:205278/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:193406/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:189792/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1608380/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1602144/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1600850/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:159979/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1583965/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1577254/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1001536/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1553824/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1550399/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1526827/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1523545/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1508423/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1497563/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1495515/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1483946/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1461865/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1452170/1‑59 (MQ=255)
aGTTGCTGCACGCGAGTACGGATTAAGCCGCGCAGATGCTGGCTGTAGCAGAAGACTTc  >  1:1447386/1‑59 (MQ=255)
                                               |           
AGTTGCTGCACGCGAGTACGAATTAAGCCGCGCAGATGCTGGCTATAACAGAAGACTTC  >  W3110S.gb/3643559‑3643617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A