Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R2
|
489 |
0.0 |
1652083 |
58.3% |
963164 |
55.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,819,124 |
+T |
intergenic (‑135/‑123) |
gmk ← / → ligB |
guanylate kinase/DNA ligase, NAD(+)‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,819,121 | 1 | . | T | 100.0%
| 14.2
/ NA
| 7 | intergenic (‑132/‑126) | gmk/ligB | guanylate kinase/DNA ligase, NAD(+)‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (7/0); total (7/0) |
CTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCA‑TTTGCCTGTCA > W3110S.gb/3819073‑3819132
|
cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTc > 1:1607409/1‑60 (MQ=255)
cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTCa > 1:1170049/1‑61 (MQ=255)
cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTCa > 1:130207/1‑61 (MQ=255)
cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTCa > 1:1337788/1‑61 (MQ=255)
cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTCa > 1:1460092/1‑61 (MQ=255)
cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTCa > 1:713507/1‑61 (MQ=255)
cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTCa > 1:798501/1‑61 (MQ=255)
|
CTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCA‑TTTGCCTGTCA > W3110S.gb/3819073‑3819132
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
