Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R2
|
489 |
0.0 |
1652083 |
58.3% |
963164 |
55.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,250,996 |
2 bp→CA |
coding (621‑622/1116 nt) |
malK → |
fused maltose transport subunit, ATP‑binding component of ABC superfamily and regulatory protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,250,996 | 0 | G | C | 100.0%
| 17.4
/ NA
| 13 | L207L (CTG→CTC) | malK | fused maltose transport subunit, ATP‑binding component of ABC superfamily and regulatory protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (0/13); total (0/13) |
| * | W3110S.gb | 4,250,997 | 0 | G | A | 100.0%
| 17.3
/ NA
| 13 | D208N (GAC→AAC) | malK | fused maltose transport subunit, ATP‑binding component of ABC superfamily and regulatory protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/13); total (0/13) |
CCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCG > W3110S.gb/4250948‑4251009
||
ccACGACCAGGTCGAGGCGATGACCCTGGCTGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:688307/62‑1 (MQ=16)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:108632/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:1309460/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:1427253/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:1604304/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:397813/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:499759/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:629107/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:673187/62‑1 (MQ=12)
ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:852803/62‑1 (MQ=12)
cACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:390714/61‑1 (MQ=12)
cACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:806145/61‑1 (MQ=12)
aCGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:417434/60‑1 (MQ=12)
||
CCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCG > W3110S.gb/4250948‑4251009
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
