Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R1
|
58 |
121.6 |
3649812 |
78.1% |
2850503 |
56.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,005,397 |
T→C |
D118G (GAC→GGC) |
hybC ← |
hydrogenase 2, large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,005,397 | 0 | T | C | 100.0%
| 21.7
/ NA
| 7 | D118G (GAC→GGC) | hybC | hydrogenase 2, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/0); total (7/0) |
AAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGT > minE/2005389‑2005442
|
aaGTGATGCCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:1466238/1‑54 (MQ=255)
aaGTGATGCCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:2271090/1‑54 (MQ=255)
aaGTGATGCCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:2303494/1‑54 (MQ=255)
aaGTGATGCCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:3110193/1‑54 (MQ=255)
aaGTGATGCCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:3365208/1‑54 (MQ=255)
aaGTGATGCCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:440168/1‑54 (MQ=255)
aaGTGATGCCCACCCAGTCAAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:1814614/1‑54 (MQ=255)
|
AAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGT > minE/2005389‑2005442
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
