Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R1
|
58 |
121.6 |
3649812 |
78.1% |
2850503 |
56.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,931,909 |
(ATC)2→3 |
coding (615/999 nt) |
idnR ← |
DNA‑binding transcriptional repressor, 5‑gluconate‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,931,902 | 1 | . | C | 100.0%
| 115.2
/ NA
| 31 | L208V (CTG→GTG) | idnR | DNA‑binding transcriptional repressor, 5‑gluconate‑binding |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (31/0); total (31/0) |
| * | minE | 2,931,902 | 2 | . | A | 100.0%
| 96.6
/ NA
| 31 | L208L (CTG→TTG) | idnR | DNA‑binding transcriptional repressor, 5‑gluconate‑binding |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (31/0); total (31/0) |
| * | minE | 2,931,902 | 3 | . | T | 100.0%
| 106.0
/ NA
| 31 | L208M (CTG→ATG) | idnR | DNA‑binding transcriptional repressor, 5‑gluconate‑binding |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (31/0); total (31/0) |
CGTGGATTCATGCGTAATGGGGAAAGATTATGCAG‑‑‑CATCATCGCATCGCAATACCCCT > minE/2931868‑2931925
|||
cgTGGATTCATGCGTAATGGGGAAAGATTATgcag‑‑‑ca > 1:2077764/1‑37 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:3106821/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:2699868/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:2737207/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:2804073/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:2819233/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:2823413/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:299216/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:2993012/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:2694848/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:3310480/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:3362760/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:3563747/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:3644636/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:451008/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:484691/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:565376/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:1337352/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:2668439/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:2491412/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:2458086/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:2437841/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:2094686/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:200039/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:1954273/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:185123/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:1781966/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:1519692/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:1474184/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:1418669/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:1397635/1‑61 (MQ=255)
cgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCATCGCATCGCAATACCCCt > 1:1357061/1‑61 (MQ=255)
|||
CGTGGATTCATGCGTAATGGGGAAAGATTATGCAG‑‑‑CATCATCGCATCGCAATACCCCT > minE/2931868‑2931925
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
