Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
385,948 |
T→A |
10.8% |
D386E (GAT→GAA) |
cstA → |
carbon starvation protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 385,948 | 0 | T | A | 10.8%
| 174.6
/ 12.3
| 65 | D386E (GAT→GAA) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base T (31/27); new base A (4/3); total (35/30) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 |
TCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGT > minE/385878‑386016
|
tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt > 1:2968835/1‑71 (MQ=255)
tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTACGCCTGTATCATCGAt < 1:174871/71‑1 (MQ=255)
cGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt < 1:1027440/70‑1 (MQ=255)
ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATc < 1:2534966/70‑1 (MQ=255)
ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc < 1:1272316/71‑1 (MQ=255)
cTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCgg > 1:1846836/1‑71 (MQ=255)
cGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCg < 1:2697598/71‑1 (MQ=255)
ggATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCgg < 1:2117396/63‑1 (MQ=255)
aTGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt > 1:2733142/1‑71 (MQ=255)
tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAtttt < 1:3409863/71‑1 (MQ=255)
tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTCTCCGCCTGTATCATCGATcc > 1:743209/1‑58 (MQ=255)
gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCggg < 1:1559909/60‑1 (MQ=255)
gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt > 1:3677136/1‑69 (MQ=255)
aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt > 1:617591/1‑66 (MQ=255)
aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt < 1:1664047/66‑1 (MQ=255)
aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGcc > 1:406668/1‑70 (MQ=255)
aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAt > 1:2331529/1‑71 (MQ=255)
tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGc > 1:2330920/1‑56 (MQ=255)
tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:3649642/71‑1 (MQ=255)
tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:1408146/71‑1 (MQ=255)
gtCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACa < 1:2450192/70‑1 (MQ=255)
aTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg > 1:1329927/1‑67 (MQ=255)
aTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGa > 1:1494920/1‑68 (MQ=255)
aTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACa < 1:2018197/71‑1 (MQ=255)
tCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGc > 1:3828925/1‑51 (MQ=255)
tCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTg < 1:2107254/61‑1 (MQ=255)
cTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGcc < 1:1425091/71‑1 (MQ=255)
cTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACac > 1:2351686/1‑68 (MQ=255)
tCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCgt > 1:3333672/1‑49 (MQ=255)
tCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc > 1:1677542/1‑70 (MQ=255)
cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc < 1:3012109/69‑1 (MQ=255)
cGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc > 1:3528104/1‑70 (MQ=255)
gTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAtt < 1:3694265/52‑1 (MQ=255)
gTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:3506746/60‑1 (MQ=255)
ttATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGt > 1:2256305/1‑70 (MQ=255)
ttATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGt > 1:3021694/1‑70 (MQ=255)
ttATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTg < 1:14396/71‑1 (MQ=255)
ttATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGaa > 1:1355696/1‑55 (MQ=255)
ttATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGaa > 1:3722328/1‑55 (MQ=255)
tATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAAc < 1:2112680/55‑1 (MQ=255)
aTGGCGCTGGTTTCCGCCTGTATCATCGATCCggg > 1:3884738/1‑35 (MQ=255)
aTGGCGCTGGTTTCCGCCTGTATCATCGATCCggg > 1:808085/1‑35 (MQ=255)
aTGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGa < 1:1431758/52‑1 (MQ=255)
tGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGt < 1:2764697/67‑1 (MQ=255)
cgcTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAGCAGCCCGATGGCGGTGCTGGCt < 1:724819/71‑1 (MQ=255)
cgcTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCt < 1:449226/71‑1 (MQ=255)
gcTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTc > 1:1405036/1‑71 (MQ=255)
cTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:110534/45‑1 (MQ=255)
cTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAAGAACAGcc > 1:2128327/1‑52 (MQ=255)
gTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCgg < 1:3141410/70‑1 (MQ=255)
tCCGCCTGTATCATCGATCCGGGCGTGTATTTTGcc > 1:1259441/1‑36 (MQ=255)
ccGCCTGTATCATCGATCCGGGCGTGTATTTTGcc > 1:375767/1‑35 (MQ=255)
ccGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAgg > 1:2988646/1‑70 (MQ=255)
cGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAggg < 1:1597973/70‑1 (MQ=255)
cGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGa > 1:254284/1‑71 (MQ=255)
cGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGa > 1:3278000/1‑71 (MQ=255)
ccTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGAcg < 1:285236/71‑1 (MQ=255)
gTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGAcggcg > 1:3698815/1‑71 (MQ=255)
tATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGc < 1:2039867/54‑1 (MQ=255)
tATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGc < 1:1562283/54‑1 (MQ=255)
tATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGAcggcg < 1:2980094/70‑1 (MQ=255)
catcGATCCGTGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGAtg > 1:2472677/1‑71 (MQ=255)
atcGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCgg > 1:2122624/1‑55 (MQ=255)
cGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTgg > 1:3672266/1‑71 (MQ=255)
cGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTgg > 1:1207560/1‑71 (MQ=255)
gATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGc > 1:2911890/1‑53 (MQ=255)
gATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCa > 1:547902/1‑54 (MQ=255)
gATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTgg < 1:3684144/70‑1 (MQ=255)
tCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGt < 1:3016322/69‑1 (MQ=255)
|
TCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGT > minE/385878‑386016
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
