Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R1 2169 63.0 3952800 76.2% 3012033 62.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 608,148 A→G 17.4% G702G (GGT→GGC)  pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE608,1480AG17.4% 243.2 / 43.1 109G702G (GGT→GGC) pflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (39/51);  new base G (2/17);  total (41/68)
Fisher's exact test for biased strand distribution p-value = 8.25e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

Only 100 of 123 total aligned reads displayed.

ATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCG  >  minE/608078‑608217
                                                                      |                                                                     
atTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacca                                                                       >  1:564393/1‑71 (MQ=255)
 tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccac                                                                      <  1:2149654/71‑1 (MQ=255)
  ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc                                                                     >  1:855116/1‑71 (MQ=255)
  ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc                                                                     >  1:534188/1‑71 (MQ=255)
  ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc                                                                     >  1:3251582/1‑71 (MQ=255)
   tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccgcc                                                                     <  1:3278536/70‑1 (MQ=255)
       cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCga                                                                <  1:868636/71‑1 (MQ=255)
       cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCga                                                                <  1:1278827/71‑1 (MQ=255)
        gggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgat                                                               <  1:1627687/71‑1 (MQ=255)
          gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg                                                              >  1:1553947/1‑70 (MQ=255)
           ttttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatgga                                                            >  1:1659397/1‑71 (MQ=255)
              tCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGc                                                         >  1:3689004/1‑71 (MQ=255)
               ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTGAGGTGCTGACCACCTTCGATGGATGc                                                         <  1:3419300/70‑1 (MQ=255)
                cATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCtt                                                       >  1:3171065/1‑71 (MQ=255)
                 aTCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCtt                                                                   >  1:1568133/1‑58 (MQ=255)
                 aTCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCtt                                                                   >  1:1577473/1‑58 (MQ=255)
                      gTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                                                 >  1:2158204/1‑71 (MQ=255)
                       tCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCga                                                                <  1:2830291/55‑1 (MQ=255)
                         gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg                                               <  1:1361846/70‑1 (MQ=255)
                         gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg                                               <  1:1775066/70‑1 (MQ=255)
                         gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg                                               >  1:1551415/1‑70 (MQ=255)
                         cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGtggtgg                                               <  1:3562883/69‑1 (MQ=255)
                         cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGa                                              <  1:1099635/70‑1 (MQ=255)
                         cagcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGa                                              <  1:1307701/70‑1 (MQ=255)
                          agcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccgcc                                                                     <  1:1502184/47‑1 (MQ=255)
                          agcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaa                                             <  1:2845961/71‑1 (MQ=12)
                           gcagcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaaa                                            <  1:2209198/71‑2 (MQ=255)
                            cagcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGt                                           <  1:410139/71‑1 (MQ=255)
                              gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                         >  1:686513/1‑71 (MQ=255)
                              gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                         >  1:690240/1‑71 (MQ=255)
                                 tttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                                                 <  1:3859320/60‑1 (MQ=255)
                                 tttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAcca                                      >  1:653763/1‑71 (MQ=255)
                                  ttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccat                                     >  1:1077683/1‑71 (MQ=255)
                                   tCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggat                                                           <  1:2052940/48‑1 (MQ=255)
                                    cACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt                                                 >  1:923323/1‑57 (MQ=255)
                                     aCGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc                                   <  1:1859894/70‑1 (MQ=255)
                                     aCGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc                                   <  1:597418/70‑1 (MQ=255)
                                      cGGTTCATCACGTTAACGTTCAGGTGCTTACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                 <  1:3467065/71‑1 (MQ=255)
                                      cGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                 <  1:103763/71‑1 (MQ=255)
                                        gTTCATCACGTTAACGTTCAGGTTCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                               <  1:1865490/71‑1 (MQ=255)
                                        gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                               <  1:3007658/71‑1 (MQ=255)
                                        gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                               <  1:743175/71‑1 (MQ=255)
                                        gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                               <  1:1093349/71‑1 (MQ=255)
                                         ttCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa                               <  1:400357/70‑1 (MQ=255)
                                           catcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                            >  1:2114380/1‑71 (MQ=255)
                                            atcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                            <  1:1054824/70‑1 (MQ=255)
                                             ttacgttgacgttgAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGccg                          <  1:3135964/69‑2 (MQ=255)
                                             tcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAcca                          <  1:3408674/71‑1 (MQ=255)
                                              tacgttgacgttgAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaa                                             >  1:556602/2‑51 (MQ=255)
                                               aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                        >  1:21870/1‑71 (MQ=255)
                                               aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                        >  1:1065758/1‑71 (MQ=255)
                                               aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagc                        >  1:2987300/1‑71 (MQ=255)
                                               aCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag                         <  1:1483649/70‑1 (MQ=255)
                                                cGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGaa                                             >  1:2506238/1‑49 (MQ=255)
                                                 gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                      <  1:1246005/71‑1 (MQ=255)
                                                 gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                      <  1:10364/71‑1 (MQ=255)
                                                 gTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccagcca                      <  1:1102502/71‑1 (MQ=255)
                                                  ttAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGt                                           <  1:2996603/49‑1 (MQ=255)
                                                  ttAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                         >  1:1356578/1‑51 (MQ=255)
                                                   tAACGTTCAGGTGCTGACCACCTTCGTTGGATGCtt                                                       <  1:2838288/36‑1 (MQ=255)
                                                   tAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAc                                        >  1:3099265/1‑51 (MQ=255)
                                                   tAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAc                                        >  1:448236/1‑51 (MQ=255)
                                                    aaCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc                                   >  1:1893065/1‑55 (MQ=255)
                                                    aaCGTTCAGGGGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTa                                          <  1:3781202/48‑1 (MQ=255)
                                                     aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                  >  1:1799271/1‑71 (MQ=255)
                                                     aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                  <  1:3703610/71‑1 (MQ=255)
                                                      cGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTg                 <  1:3223304/71‑1 (MQ=255)
                                                      cGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTg                 <  1:2482210/71‑1 (MQ=255)
                                                       gTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                  >  1:3239501/1‑69 (MQ=255)
                                                        ttCAGGTGCTGACCACCTTCGATGGATGCTTCgtgg                                                  <  1:2320480/36‑1 (MQ=255)
                                                        ttCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                                 <  1:1175710/53‑1 (MQ=255)
                                                        ttCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAc                            <  1:3874000/58‑1 (MQ=255)
                                                         tCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc              >  1:1994647/1‑71 (MQ=255)
                                                          gaGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGttt             >  1:2508660/2‑69 (MQ=255)
                                                          cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc              <  1:3159160/70‑1 (MQ=255)
                                                          cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTc              >  1:1792913/1‑70 (MQ=255)
                                                          cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt             >  1:401254/1‑71 (MQ=255)
                                                          cAGGTGCTGACCACCTTCGATGGATGCTTCGTGGT‑GAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt             >  1:2402537/1‑70 (MQ=255)
                                                           aGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGtttt            <  1:942347/71‑1 (MQ=255)
                                                           aGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGtttt            <  1:2231230/71‑1 (MQ=255)
                                                           aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa                                         >  1:2491314/1‑42 (MQ=255)
                                                           aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt             <  1:2804426/70‑1 (MQ=255)
                                                           aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt             <  1:2700611/70‑1 (MQ=255)
                                                           aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt             <  1:2689224/70‑1 (MQ=255)
                                                           aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt             <  1:2362760/70‑1 (MQ=255)
                                                           aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt             <  1:2343644/70‑1 (MQ=255)
                                                           aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAACCTGGCTGGTCt             <  1:3119832/70‑1 (MQ=255)
                                                            ggTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTa           <  1:3824925/71‑1 (MQ=255)
                                                            ggTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTa           <  1:3409193/71‑1 (MQ=255)
                                                            ggTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACAATCCATCAGACCAGCCAGGTTGGTCTTa           <  1:3880655/71‑1 (MQ=255)
                                                             gtgtTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACccatcca                                  <  1:1868925/47‑1 (MQ=255)
                                                             gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc          <  1:1251432/71‑1 (MQ=255)
                                                             gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc          >  1:923689/1‑71 (MQ=255)
                                                             gTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc          <  1:1993034/71‑1 (MQ=255)
                                                               gCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACg         >  1:2724014/1‑70 (MQ=255)
                                                                   accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAAc      <  1:3631156/69‑1 (MQ=255)
                                                                   accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt     <  1:2984463/70‑1 (MQ=255)
                                                                    ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc          >  1:2649502/1‑64 (MQ=255)
                                                                    ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTAc          >  1:1479166/1‑64 (MQ=255)
                                                                    ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc   >  1:2907297/1‑71 (MQ=255)
                                                                      |                                                                     
ATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCG  >  minE/608078‑608217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A