Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
608,316 |
T→C |
7.7% |
V646V (GTA→GTG) |
pflB ← |
pyruvate formate lyase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 608,316 | 0 | T | C | 7.7%
| 179.1
/ 5.4
| 65 | V646V (GTA→GTG) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base T (27/33); new base C (2/3); total (29/36) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 |
AGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGAC > minE/608249‑608386
|
agaAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACt > 1:2131970/1‑71 (MQ=255)
aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTg > 1:1123783/1‑70 (MQ=255)
gtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCtt > 1:953217/1‑71 (MQ=255)
tacgagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCttt < 1:1753908/68‑1 (MQ=255)
ggAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTc < 1:2401660/70‑1 (MQ=255)
aTACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTc < 1:1654394/71‑1 (MQ=255)
aCCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTc > 1:1331851/1‑69 (MQ=255)
cATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGa > 1:720036/1‑71 (MQ=255)
aTCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACATTTCTGGTCACGAc > 1:3260619/1‑71 (MQ=255)
tCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGAc > 1:1051499/1‑70 (MQ=255)
tttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGt > 1:1980820/1‑71 (MQ=255)
tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGTACCTTTCTGGTCACGACCGTg > 1:87529/1‑70 (MQ=255)
tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAcc < 1:2037412/67‑1 (MQ=255)
cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc < 1:3250035/71‑1 (MQ=255)
cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTAACGACCGTGc < 1:913851/68‑1 (MQ=255)
cGTAAGAAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc < 1:3164314/71‑1 (MQ=255)
tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTc < 1:3559758/51‑1 (MQ=255)
tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCgg > 1:1019022/1‑71 (MQ=255)
tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTAATg < 1:355408/43‑1 (MQ=255)
aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGc > 1:2251034/1‑42 (MQ=255)
aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCATCTTTCTGGTCACGACCGTGCATCGGGt < 1:3696793/71‑1 (MQ=255)
aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGt > 1:850934/1‑71 (MQ=255)
aGCAAACGGCAGTTTAGCAACGGAAGGCAGCGCGGCTACTGCACCTTTCTGGTCACGACCGTGCATCggg < 1:3344432/70‑1 (MQ=255)
gtgaaCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGtt < 1:2517230/68‑1 (MQ=255)
gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGt < 1:1104843/52‑1 (MQ=255)
gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCgg > 1:1086037/1‑68 (MQ=255)
gCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGtt < 1:3646045/71‑1 (MQ=255)
aaCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTAc > 1:1135848/1‑35 (MQ=255)
aaCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCa > 1:1794823/1‑39 (MQ=255)
aaCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc < 1:1799456/63‑1 (MQ=255)
aCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCa < 1:552843/71‑1 (MQ=255)
cGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcgc > 1:2072388/1‑69 (MQ=255)
ggCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAcc < 1:1770690/71‑1 (MQ=255)
aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg < 1:539325/71‑1 (MQ=255)
aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg < 1:1867519/71‑1 (MQ=255)
gTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCCTGCATCGGGTTAGCGCCCggc < 1:3263540/71‑2 (MQ=255)
tttAGCATCGGAAGTCTGAGAGGCTACTGCACCTTTc < 1:2397951/37‑1 (MQ=255)
tttAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAcc < 1:2470539/48‑1 (MQ=255)
tttAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc > 1:3031956/1‑71 (MQ=255)
tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc > 1:2954541/1‑70 (MQ=255)
tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc > 1:3643701/1‑70 (MQ=255)
tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTcc > 1:878034/1‑70 (MQ=255)
tAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTc > 1:2297856/1‑69 (MQ=255)
aGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAcc > 1:323299/1‑63 (MQ=255)
gCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGa < 1:1527459/70‑1 (MQ=255)
aaCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACg < 1:2364158/71‑1 (MQ=255)
aCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACgg > 1:3186160/1‑71 (MQ=255)
aCGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACg < 1:3366397/70‑1 (MQ=255)
cGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgc > 1:1922/1‑71 (MQ=255)
ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcg > 1:3165444/1‑71 (MQ=255)
aaGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGc < 1:751043/52‑1 (MQ=255)
aaGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGc < 1:1505466/52‑1 (MQ=255)
aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc > 1:779293/1‑71 (MQ=255)
gTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCg < 1:2161746/55‑1 (MQ=255)
agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGtt > 1:546803/1‑44 (MQ=255)
agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCa < 1:1961746/69‑1 (MQ=255)
agagagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGc < 1:2220336/71‑1 (MQ=255)
agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac < 1:2923959/71‑1 (MQ=255)
gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGtt < 1:506275/41‑1 (MQ=255)
gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGaa < 1:3905117/56‑1 (MQ=255)
gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac > 1:696155/1‑70 (MQ=255)
gagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac > 1:1172352/1‑70 (MQ=255)
agGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGt < 1:2689728/39‑1 (MQ=255)
ggCTACTGCACCTTTCTGGACACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacgac < 1:3200847/71‑1 (MQ=255)
gCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac < 1:836964/67‑1 (MQ=255)
tACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACgacgac > 1:3728350/1‑71 (MQ=255)
|
AGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGAC > minE/608249‑608386
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
