Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
835,378 |
C→G |
25.2% |
S32R (AGC→AGG) ‡ |
narH → |
nitrate reductase 1, beta (Fe‑S) subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 835,378 | 0 | C | G | 25.2%
| 151.8
/ 48.8
| 87 | S32R (AGC→AGG) ‡ | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base C (32/33); new base G (11/11); total (43/44) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACT > minE/835309‑835448
|
ggTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGcc > 1:52784/1‑71 (MQ=255)
aacctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGaa < 1:2160995/66‑1 (MQ=255)
aacctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAg > 1:1130916/4‑70 (MQ=255)
aacctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAg > 1:2376194/4‑70 (MQ=255)
aTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGc < 1:3043866/71‑1 (MQ=255)
aTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGc < 1:2687975/71‑1 (MQ=255)
aTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGc < 1:70033/71‑1 (MQ=255)
cctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGca < 1:3542248/70‑2 (MQ=255)
tcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:3842024/1‑62 (MQ=255)
cGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTg > 1:1826224/1‑70 (MQ=255)
gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGa < 1:1793399/71‑1 (MQ=255)
aTAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGaa < 1:3952560/71‑1 (MQ=255)
aaGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAata > 1:2958253/1‑71 (MQ=255)
aaGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAata > 1:2162018/1‑71 (MQ=255)
aGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAata < 1:931629/70‑1 (MQ=255)
aGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAata < 1:2416959/70‑1 (MQ=255)
aGTGCATCGGCTG‑CACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATg > 1:2513863/1‑71 (MQ=255)
gTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAatat > 1:1832505/1‑70 (MQ=255)
tGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGc < 1:2742999/71‑1 (MQ=255)
tGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGc < 1:2472128/71‑1 (MQ=255)
cATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGt > 1:2047527/1‑71 (MQ=255)
cATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGt > 1:3270565/1‑71 (MQ=255)
aTCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTg < 1:69819/71‑1 (MQ=255)
ggCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGtt > 1:430549/1‑71 (MQ=255)
ggCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGt < 1:2436538/70‑1 (MQ=255)
gCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGttt < 1:109/71‑2 (MQ=255)
gCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGtt > 1:2297446/1‑70 (MQ=255)
gCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTc < 1:2074764/71‑1 (MQ=255)
cTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGttt < 1:3108489/70‑2 (MQ=255)
tGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaa > 1:2051205/1‑71 (MQ=255)
tGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaa > 1:2398714/1‑71 (MQ=255)
tGCCACACCTGCTCGATCACCTGCAAGAACGTCTGGACCAGCCGCGAAGGCATGGAGTACGCCTGGTTcaa > 1:709105/1‑71 (MQ=255)
ccATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTtaac > 1:1854520/1‑70 (MQ=255)
ccATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTtaac > 1:2892066/1‑70 (MQ=255)
ccACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaa < 1:3337043/69‑1 (MQ=255)
tacCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACg < 1:2538245/70‑1 (MQ=255)
cacCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATg > 1:3634736/1‑56 (MQ=255)
cacCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACg < 1:2316976/71‑1 (MQ=255)
cacCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACg < 1:2439287/71‑1 (MQ=255)
acCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGt > 1:2166895/1‑71 (MQ=255)
cTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATg > 1:1677135/1‑46 (MQ=255)
cTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAat > 1:3027312/1‑50 (MQ=255)
cTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaaca > 1:1893268/1‑65 (MQ=255)
cTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTgg < 1:1327911/71‑1 (MQ=255)
cTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTg > 1:3401816/1‑70 (MQ=255)
tGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATg > 1:1440941/1‑52 (MQ=255)
ttgaGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGaa < 1:2134511/67‑1 (MQ=255)
ttCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGaaa > 1:2118523/1‑71 (MQ=255)
ttCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGaa < 1:243508/70‑1 (MQ=255)
ttCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGa < 1:2919966/69‑1 (MQ=255)
ttCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTca < 1:3307356/59‑1 (MQ=255)
ttCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGa < 1:3215110/69‑1 (MQ=255)
tCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaac > 1:1884875/1‑60 (MQ=255)
tCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaac > 1:699739/1‑60 (MQ=255)
cAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGaa > 1:1486749/1‑44 (MQ=255)
cAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAAc < 1:3762977/70‑1 (MQ=255)
aGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCa < 1:1729499/71‑1 (MQ=255)
gTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAAcc > 1:2873644/1‑69 (MQ=255)
gTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCaa < 1:3256987/71‑1 (MQ=255)
ttACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTca < 1:1815241/54‑1 (MQ=255)
ttACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAAcc < 1:2143466/68‑1 (MQ=255)
ttACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAg < 1:942218/71‑1 (MQ=255)
tgaCCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACg < 1:3412602/58‑1 (MQ=255)
aCCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGtc > 1:2752404/1‑59 (MQ=255)
aCCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGaaaccaaacc > 1:690504/1‑71 (MQ=255)
aCCTGTAAAAACGTCTGGACCGGGCGAGAAGGCATGGAGTACGCATGGTTTAACAACGtc > 1:3463912/1‑59 (MQ=255)
aCCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCaa < 1:1861070/68‑1 (MQ=255)
aCCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGcc > 1:3361058/1‑71 (MQ=255)
ccTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGcc < 1:2274587/70‑1 (MQ=255)
cTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGtc < 1:2574569/58‑2 (MQ=255)
tGTAAAAACGTCTGGACCTGGCGCGAAGGCATGGAgtac < 1:1932449/39‑2 (MQ=16)
ttAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCggg > 1:3332404/2‑70 (MQ=255)
tAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTaacaac < 1:1343900/52‑1 (MQ=255)
tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaacaa > 1:2718493/1‑51 (MQ=255)
tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGcc < 1:3938006/71‑1 (MQ=255)
tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGcc < 1:188282/71‑1 (MQ=255)
tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGc > 1:1180552/1‑70 (MQ=255)
aaaaCGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCAAACCGGGcatt > 1:1561972/1‑68 (MQ=255)
aaaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCa < 1:3446346/70‑1 (MQ=255)
aCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaac > 1:1236055/1‑44 (MQ=255)
aCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaac > 1:1052481/1‑44 (MQ=255)
aCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGc > 1:1035147/1‑71 (MQ=255)
aCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGc > 1:2327977/1‑71 (MQ=255)
aCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAAGGc < 1:1573352/71‑1 (MQ=255)
cGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCt < 1:1203280/71‑1 (MQ=255)
cTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCa > 1:2080787/1‑63 (MQ=255)
gACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTccc < 1:2819519/69‑1 (MQ=255)
gCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgac > 1:3545396/1‑71 (MQ=255)
ccGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCa < 1:2673428/44‑1 (MQ=255)
ccGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgact < 1:1795505/71‑1 (MQ=255)
ccGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgact > 1:3544092/1‑71 (MQ=255)
|
GGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACT > minE/835309‑835448
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
